CCDA_BACSU
ID CCDA_BACSU Reviewed; 235 AA.
AC P45706;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Cytochrome c-type biogenesis protein CcdA;
GN Name=ccdA; OrderedLocusNames=BSU17930;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969507; DOI=10.1099/13500872-142-11-3097;
RA Rose M., Entian K.-D.;
RT "New genes in the 170 degrees region of the Bacillus subtilis genome encode
RT DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid
RT transporter.";
RL Microbiology 142:3097-3101(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9068642; DOI=10.1128/jb.179.6.1962-1973.1997;
RA Schioett T., von Wachenfeldt C., Hederstedt L.;
RT "Identification and characterization of the ccdA gene, required for
RT cytochrome c synthesis in Bacillus subtilis.";
RL J. Bacteriol. 179:1962-1973(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=168;
RX PubMed=10781554; DOI=10.1128/jb.182.10.2845-2854.2000;
RA Schioett T., Hederstedt L.;
RT "Efficient spore synthesis in Bacillus subtilis depends on the CcdA
RT protein.";
RL J. Bacteriol. 182:2845-2854(2000).
RN [5]
RP SUPPRESSION OF CCDA-DEFICIENCY.
RC STRAIN=168 / BGSC1A1;
RX PubMed=11844773; DOI=10.1128/jb.184.5.1423-1429.2002;
RA Erlendsson L.S., Hederstedt L.;
RT "Mutations in the thiol-disulfide oxidoreductases BdbC and BdbD can
RT suppress cytochrome c deficiency of CcdA-defective Bacillus subtilis
RT cells.";
RL J. Bacteriol. 184:1423-1429(2002).
CC -!- FUNCTION: Required for cytochrome c synthesis and stage V of
CC sporulation. Might transfer reducing equivalents across the cytoplasmic
CC membrane, promoting efficient disulfide bond isomerization of proteins
CC localized on the outer surface of the membrane or in the spore coat.
CC {ECO:0000269|PubMed:10781554}.
CC -!- FUNCTION: Cytochrome c oxidase activity is partially restored by
CC mutations in bdbC or bdbD that are defective in the synthesis of
CC disulfide bond containing proteins. The same mutations abrogate the
CC need for CcdA in sporulation. {ECO:0000269|PubMed:10781554}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10781554};
CC Multi-pass membrane protein {ECO:0000269|PubMed:10781554}.
CC -!- SIMILARITY: Belongs to the DsbD family. {ECO:0000305}.
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DR EMBL; Z73234; CAA97594.1; -; Genomic_DNA.
DR EMBL; X87845; CAA61116.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13677.1; -; Genomic_DNA.
DR PIR; D69597; D69597.
DR RefSeq; NP_389676.1; NC_000964.3.
DR RefSeq; WP_003231585.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P45706; -.
DR SMR; P45706; -.
DR STRING; 224308.BSU17930; -.
DR TCDB; 5.A.1.2.1; the disulfide bond oxidoreductase d (dsbd) family.
DR PaxDb; P45706; -.
DR DNASU; 937140; -.
DR EnsemblBacteria; CAB13677; CAB13677; BSU_17930.
DR GeneID; 937140; -.
DR KEGG; bsu:BSU17930; -.
DR PATRIC; fig|224308.179.peg.1954; -.
DR eggNOG; COG0785; Bacteria.
DR InParanoid; P45706; -.
DR OMA; LYFDWMT; -.
DR PhylomeDB; P45706; -.
DR BioCyc; BSUB:BSU17930-MON; -.
DR BioCyc; MetaCyc:BSU17930-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR003834; Cyt_c_assmbl_TM_dom.
DR Pfam; PF02683; DsbD; 1.
PE 3: Inferred from homology;
KW Cell membrane; Cytochrome c-type biogenesis; Membrane; Reference proteome;
KW Sporulation; Transmembrane; Transmembrane helix.
FT CHAIN 1..235
FT /note="Cytochrome c-type biogenesis protein CcdA"
FT /id="PRO_0000201624"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 165..185
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 235 AA; 26007 MW; D94F3E62EDD2AC29 CRC64;
MGDVNYFLTF GAGFLSFISP CCLPLYPAFL SYITGVSMDD VKTEKLLLQK RSLFHTLCFL
LGFSVIFIAL GYGTSFIGSL FRDYHDAIRQ IGALLIILFG FITLGVFRPE AMMKERRIHF
KHKPSGFLGS VLIGMAFAAG WTPCTGPILA AVITLAGTNP GSAVPYMMLY VLGFAVPFLL
LSFFITKLKW IRKNQLFIMK AGGVLMIVIG VLLFFNWMSL IIILLSDLFG GFTGF
