CCDA_ECO57
ID CCDA_ECO57 Reviewed; 72 AA.
AC P62553; P05702;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Antitoxin CcdA;
DE AltName: Full=LynA;
DE AltName: Full=Protein H;
DE AltName: Full=Protein LetA;
GN Name=ccdA; Synonyms=H, letA; OrderedLocusNames=L7062, ECO57PM27;
OS Escherichia coli O157:H7.
OG Plasmid pO157.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=9722640; DOI=10.1093/nar/26.18.4196;
RA Burland V., Shao Y., Perna N.T., Plunkett G. III, Sofia H.J.,
RA Blattner F.R.;
RT "The complete DNA sequence and analysis of the large virulence plasmid of
RT Escherichia coli O157:H7.";
RL Nucleic Acids Res. 26:4196-4204(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=9628576; DOI=10.1093/dnares/5.1.1;
RA Makino K., Ishii K., Yasunaga T., Hattori M., Yokoyama K., Yatsudo H.C.,
RA Kubota Y., Yamaichi Y., Iida T., Yamamoto K., Honda T., Han C.G.,
RA Ohtsubo A., Kasamatsu M., Hayashi T., Kuhara S., Shinagawa H.;
RT "Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an
RT enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak.";
RL DNA Res. 5:1-9(1998).
CC -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system
CC which inhibits the post-segregational killing (PSK) of plasmid-free
CC cells, also referred to as a plasmid addiction system. Binds to and
CC blocks the activity of CcdB; will also remove bound CcdB protein from
CC the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed
CC rejuvenation. Functions as a transcriptional corepressor for the ccdAB
CC operon, repression also requires CcdB (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer in solution and when bound to DNA. Forms a complex
CC with toxin CcdB; the CcdA-CcdB(2) trimer is sufficient for
CC rejuvenation, whereas maximal operon repression occurs with
CC CcdA(2)CcdB(2) (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CcdA antitoxin family. {ECO:0000305}.
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DR EMBL; AF074613; AAC70130.1; -; Genomic_DNA.
DR EMBL; AB011549; BAA31784.1; -; Genomic_DNA.
DR RefSeq; NP_052634.1; NC_002128.1.
DR RefSeq; WP_000813634.1; NZ_SEKS01000035.1.
DR PDB; 3TCJ; X-ray; 1.93 A; T=37-72.
DR PDBsum; 3TCJ; -.
DR AlphaFoldDB; P62553; -.
DR SMR; P62553; -.
DR STRING; 155864.EDL933_p0058; -.
DR EnsemblBacteria; AAC70130; AAC70130; Z_L7062.
DR EnsemblBacteria; BAA31784; BAA31784; BAA31784.
DR GeneID; 1789714; -.
DR KEGG; ece:Z_L7062; -.
DR KEGG; ecs:pO157p28; -.
DR PATRIC; fig|386585.9.peg.31; -.
DR eggNOG; COG5302; Bacteria.
DR HOGENOM; CLU_157097_1_0_6; -.
DR OMA; HHNNIYL; -.
DR Proteomes; UP000000558; Plasmid pO157.
DR Proteomes; UP000002519; Plasmid pO157.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR009956; Post-segregation_anti-tox_CcdA.
DR Pfam; PF07362; CcdA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Plasmid; Reference proteome; Repressor;
KW Toxin-antitoxin system; Transcription; Transcription regulation.
FT CHAIN 1..72
FT /note="Antitoxin CcdA"
FT /id="PRO_0000068384"
FT HELIX 41..47
FT /evidence="ECO:0007829|PDB:3TCJ"
FT HELIX 49..62
FT /evidence="ECO:0007829|PDB:3TCJ"
FT HELIX 65..69
FT /evidence="ECO:0007829|PDB:3TCJ"
SQ SEQUENCE 72 AA; 8372 MW; BFA1C8AEFEDF511B CRC64;
MKQRITVTVD SDSYQLLKAY DVNISGLVST TMQNEARRLR AERWKAENQE GMAEVARFIE
MNGSFADENR DW
