CCG3_MOUSE
ID CCG3_MOUSE Reviewed; 315 AA.
AC Q9JJV5; A6H6R5;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Voltage-dependent calcium channel gamma-3 subunit;
DE AltName: Full=Neuronal voltage-gated calcium channel gamma-3 subunit;
DE AltName: Full=Transmembrane AMPAR regulatory protein gamma-3;
DE Short=TARP gamma-3;
GN Name=Cacng3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=10734232; DOI=10.1016/s0014-5793(00)01306-5;
RA Klugbauer N., Dai S., Specht V., Lacinova L., Marais E., Bohn G.,
RA Hofmann F.;
RT "A family of gamma-like calcium channel subunits.";
RL FEBS Lett. 470:189-197(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, INTERACTION WITH AP4M1 AND GRIA1, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF 227-TYR--THR-240.
RX PubMed=18341993; DOI=10.1016/j.neuron.2008.02.012;
RA Matsuda S., Miura E., Matsuda K., Kakegawa W., Kohda K., Watanabe M.,
RA Yuzaki M.;
RT "Accumulation of AMPA receptors in autophagosomes in neuronal axons lacking
RT adaptor protein AP-4.";
RL Neuron 57:730-745(2008).
CC -!- FUNCTION: Regulates the trafficking to the somatodendritic compartment
CC and gating properties of AMPA-selective glutamate receptors (AMPARs)
CC (PubMed:18341993). Promotes their targeting to the cell membrane and
CC synapses and modulates their gating properties by slowing their rates
CC of activation, deactivation and desensitization. Does not show subunit-
CC specific AMPA receptor regulation and regulates all AMPAR subunits.
CC Thought to stabilize the calcium channel in an inactivated (closed)
CC state (By similarity). {ECO:0000250|UniProtKB:Q8VHX0,
CC ECO:0000269|PubMed:18341993}.
CC -!- SUBUNIT: The L-type calcium channel is composed of five subunits:
CC alpha-1, alpha-2/delta, beta and gamma. Acts as an auxiliary subunit
CC for AMPA-selective glutamate receptors (AMPARs). Found in a complex
CC with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG4, CACNG5,
CC CACNG7 and CACNG8 (By similarity). Interacts with AP4M1 and GRIA1;
CC associates GRIA1 with the adaptor protein complex 4 (AP-4) to target
CC GRIA1 to the somatodendritic compartment of neurons (PubMed:18341993).
CC {ECO:0000250|UniProtKB:Q8VHX0, ECO:0000269|PubMed:18341993}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Note=Displays a somatodendritic localization and
CC is excluded from axons in neurons. {ECO:0000269|PubMed:18341993}.
CC -!- SIMILARITY: Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ272044; CAB86385.1; -; mRNA.
DR EMBL; CH466531; EDL17281.1; -; Genomic_DNA.
DR EMBL; BC138673; AAI38674.1; -; mRNA.
DR EMBL; BC145971; AAI45972.1; -; mRNA.
DR CCDS; CCDS21816.1; -.
DR RefSeq; NP_062303.2; NM_019430.2.
DR AlphaFoldDB; Q9JJV5; -.
DR SMR; Q9JJV5; -.
DR IntAct; Q9JJV5; 1.
DR MINT; Q9JJV5; -.
DR STRING; 10090.ENSMUSP00000081664; -.
DR iPTMnet; Q9JJV5; -.
DR PhosphoSitePlus; Q9JJV5; -.
DR PaxDb; Q9JJV5; -.
DR PeptideAtlas; Q9JJV5; -.
DR PRIDE; Q9JJV5; -.
DR ProteomicsDB; 265607; -.
DR Antibodypedia; 26064; 237 antibodies from 28 providers.
DR DNASU; 54376; -.
DR Ensembl; ENSMUST00000084615; ENSMUSP00000081664; ENSMUSG00000066189.
DR GeneID; 54376; -.
DR KEGG; mmu:54376; -.
DR UCSC; uc009jov.3; mouse.
DR CTD; 10368; -.
DR MGI; MGI:1859165; Cacng3.
DR VEuPathDB; HostDB:ENSMUSG00000066189; -.
DR eggNOG; ENOG502QVF5; Eukaryota.
DR GeneTree; ENSGT01050000244893; -.
DR HOGENOM; CLU_053704_0_1_1; -.
DR InParanoid; Q9JJV5; -.
DR OMA; NPREPGN; -.
DR OrthoDB; 1117127at2759; -.
DR PhylomeDB; Q9JJV5; -.
DR TreeFam; TF327980; -.
DR Reactome; R-MMU-5682910; LGI-ADAM interactions.
DR BioGRID-ORCS; 54376; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q9JJV5; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9JJV5; protein.
DR Bgee; ENSMUSG00000066189; Expressed in superior frontal gyrus and 56 other tissues.
DR ExpressionAtlas; Q9JJV5; baseline and differential.
DR Genevisible; Q9JJV5; MM.
DR GO; GO:0032281; C:AMPA glutamate receptor complex; IDA:MGI.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0060076; C:excitatory synapse; ISO:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; IDA:SynGO.
DR GO; GO:0098839; C:postsynaptic density membrane; ISO:MGI.
DR GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IDA:SynGO.
DR GO; GO:0036477; C:somatodendritic compartment; IDA:UniProtKB.
DR GO; GO:0016247; F:channel regulator activity; IBA:GO_Central.
DR GO; GO:0035255; F:ionotropic glutamate receptor binding; ISO:MGI.
DR GO; GO:0030165; F:PDZ domain binding; ISO:MGI.
DR GO; GO:0005245; F:voltage-gated calcium channel activity; IBA:GO_Central.
DR GO; GO:0099590; P:neurotransmitter receptor internalization; IBA:GO_Central.
DR GO; GO:0099645; P:neurotransmitter receptor localization to postsynaptic specialization membrane; IDA:SynGO.
DR GO; GO:0098943; P:neurotransmitter receptor transport, postsynaptic endosome to lysosome; IBA:GO_Central.
DR GO; GO:2000969; P:positive regulation of AMPA receptor activity; ISO:MGI.
DR GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; IBA:GO_Central.
DR GO; GO:0098970; P:postsynaptic neurotransmitter receptor diffusion trapping; IBA:GO_Central.
DR GO; GO:0008104; P:protein localization; IMP:UniProtKB.
DR GO; GO:0006605; P:protein targeting; IMP:UniProtKB.
DR GO; GO:2000311; P:regulation of AMPA receptor activity; ISS:UniProtKB.
DR GO; GO:0019226; P:transmission of nerve impulse; IBA:GO_Central.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR InterPro; IPR008368; VDCC_gsu.
DR Pfam; PF00822; PMP22_Claudin; 1.
DR PRINTS; PR01792; VDCCGAMMA.
PE 1: Evidence at protein level;
KW Calcium; Calcium channel; Calcium transport; Ion channel; Ion transport;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..315
FT /note="Voltage-dependent calcium channel gamma-3 subunit"
FT /id="PRO_0000164676"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 232..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 248
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VHX0"
FT MUTAGEN 227..240
FT /note="YRYRFRRRSSSRST->ARARARRRAAARAA: Decreased
FT interaction with AP4M1. Has no effect on interaction with
FT GRIA1. Dominant negative mutant which alters AMPARs
FT localization to the somatodendritic compartment."
FT /evidence="ECO:0000269|PubMed:18341993"
FT CONFLICT 61
FT /note="S -> F (in Ref. 1; CAB86385)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 315 AA; 35516 MW; FB4EA036C494B6AD CRC64;
MRMCDRGIQM LITTVGAFAA FSLMTIAVGT DYWLYSRGVC RTKSTSDNET SRKNEEVMTH
SGLWRTCCLE GAFRGVCKKI DHFPEDADYE QDTAEYLLRA VRASSVFPIL SVTLLFFGGL
CVAASEFHRS RHSVILSAGI FFVSAGLSNI IGIIVYISAN AGDPGQRDSK KSYSYGWSFY
FGAFSFIIAE IVGVVAVHIY IEKHQQLRAR SHSELLKKST FARLPPYRYR FRRRSSSRST
EPRSRDLSPI SKGFHTIPST DISMFTLSRD PSKLTMGTLL NSDRDHAFLQ FHNSTPKEFK
ESLHNNPANR RTTPV
