ID   CCI1_ARATH              Reviewed;         280 AA.
AC   Q9M643;
DT   19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Cycloeucalenol cycloisomerase;
DE            EC=5.5.1.9;
DE   AltName: Full=Cycloeucalenol--obtusifoliol isomerase;
DE   AltName: Full=Cyclopropyl sterol isomerase;
GN   Name=CPI1; OrderedLocusNames=At5g50375; ORFNames=MXI22.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10788449; DOI=10.1074/jbc.275.18.13394;
RA   Lovato M.A., Hart E.A., Segura M.J.R., Giner J.-L., Matsuda S.P.T.;
RT   "Functional cloning of an Arabidopsis thaliana cDNA encoding cycloeucalenol
RT   cycloisomerase.";
RL   J. Biol. Chem. 275:13394-13397(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Converts pentacyclic cyclopropyl sterols to tetracyclic
CC       sterols.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cycloeucalenol = obtusifoliol; Xref=Rhea:RHEA:22800,
CC         ChEBI:CHEBI:16653, ChEBI:CHEBI:17791; EC=5.5.1.9;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9M643-1; Sequence=Displayed;
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DR   EMBL; AF216756; AAF67863.1; -; mRNA.
DR   EMBL; AB012248; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED95935.1; -; Genomic_DNA.
DR   RefSeq; NP_568727.1; NM_124419.5. [Q9M643-1]
DR   AlphaFoldDB; Q9M643; -.
DR   BioGRID; 20351; 6.
DR   STRING; 3702.AT5G50375.2; -.
DR   PaxDb; Q9M643; -.
DR   ProteomicsDB; 224380; -. [Q9M643-1]
DR   EnsemblPlants; AT5G50375.1; AT5G50375.1; AT5G50375. [Q9M643-1]
DR   GeneID; 835105; -.
DR   Gramene; AT5G50375.1; AT5G50375.1; AT5G50375. [Q9M643-1]
DR   KEGG; ath:AT5G50375; -.
DR   Araport; AT5G50375; -.
DR   eggNOG; ENOG502QPR8; Eukaryota.
DR   HOGENOM; CLU_061063_0_0_1; -.
DR   InParanoid; Q9M643; -.
DR   OMA; FWTHYFY; -.
DR   PhylomeDB; Q9M643; -.
DR   BioCyc; ARA:AT5G50375-MON; -.
DR   BioCyc; MetaCyc:AT5G50375-MON; -.
DR   BRENDA; 5.5.1.9; 399.
DR   PRO; PR:Q9M643; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9M643; baseline and differential.
DR   Genevisible; Q9M643; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0047793; F:cycloeucalenol cycloisomerase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR020532; Cycloeucalenol_cycloisomerase.
DR   PANTHER; PTHR35136; PTHR35136; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Isomerase; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..280
FT                   /note="Cycloeucalenol cycloisomerase"
FT                   /id="PRO_0000089422"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   280 AA;  32644 MW;  B51959FE356C48E1 CRC64;
     MSGSSSPSLW LAPNPSKRWG ELFFLFYTPF WLTLCLGIVV PYKLYETFTE LEYLLLALVS
     AVPAFVIPML LVGKADRSLC WKDRYWVKAN LWIIVFSYVG NYFWTHYFFK VLGASYTFPS
     WKMNNVPHTT FFLTHVCFLF YHVASNITLR RLRHSTADLP DSLKWCFEAA WILALSYFIA
     YLETIAIANF PYYEFVDRSA MYRVGCLFYA IYFIVSFPMF FRMDEKSTDE WDLSRVAVDA
     LGAAMLVTII LDLWRLFLGP IVPLPEGQNC LQSGLPWFSN