ID   CCL10_HUMLU             Reviewed;         543 AA.
AC   M4IQS1;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 20.
DE   RecName: Full=Probable CoA ligase CCL10 {ECO:0000305|PubMed:23300257};
DE            Short=HlCCL10 {ECO:0000303|PubMed:23300257};
DE            EC=6.2.1.- {ECO:0000305|PubMed:23300257};
GN   Name=CCL10 {ECO:0000303|PubMed:23300257};
OS   Humulus lupulus (European hop).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Humulus.
OX   NCBI_TaxID=3486;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Nugget;
RX   PubMed=23300257; DOI=10.1093/mp/sst004;
RA   Xu H., Zhang F., Liu B., Huhman D.V., Sumner L.W., Dixon R.A., Wang G.;
RT   "Characterization of the formation of branched short-chain fatty acid:CoAs
RT   for bitter acid biosynthesis in hop glandular trichomes.";
RL   Mol. Plant 6:1301-1317(2013).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:M4IRL4}.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are involved in
CC       the substrate recognition, and are sufficient to confer the substrate
CC       specificity. {ECO:0000250|UniProtKB:Q42524}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; JQ740212; AGA17927.1; -; mRNA.
DR   AlphaFoldDB; M4IQS1; -.
DR   SMR; M4IQS1; -.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016405; F:CoA-ligase activity; ISS:UniProtKB.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN           1..543
FT                   /note="Probable CoA ligase CCL10"
FT                   /id="PRO_0000452955"
FT   REGION          267..337
FT                   /note="SBD1"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   REGION          338..402
FT                   /note="SBD2"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   BINDING         196..204
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         337..342
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         423
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         435..438
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         529
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
SQ   SEQUENCE   543 AA;  60208 MW;  6687F32991CA72FB CRC64;
     MEKCFNPETQ IYSSHRPPVN FPTDPKLSLT SFLFRSSASY PNRTALIDAD SGQTLTFLKL
     KTQVSKLAHS LVQLNIKKND VVLIFAPNSI HFPVCFFSIA ALGAITTTCN PSYTFTELSN
     QAKDCNPTLV ITVPELWEKA RKLNLPAIIL PSPSNSKLSS KSEFWFFSDL TRKSDRFSEL
     PISDVRQSDV AALLYSSGTT GKSKGVVLSH KNFITTSLMV TSDQDRYGDP QNICMCFLPM
     FHIFGLSVIA YSQLRRGNGV VSMGKFELEG ALRAVEMYRV THLFVVPPVM IALAKESVVV
     RRYDLSSVKE ILSGAAPLGK NVMEQCARNV PGAAIIQGYG MTETCGIISI EDSKKGCRFS
     GSTGMLAPGI ESQIMDTKRW NPLPPNQEGE IWLRGPNMMQ GYLNNLEATK STIDEQGWVK
     TGDIGYFDEE GQLFVVDRLK ELIKCYGFQV APAELEALLL SHPEILDAVV IPFPDEKAGE
     VPIANVVRSP NSSLSEEDVQ RFIEKQVAPF KKLRRVTFVS SVLKSPAGKI LRRELIQKVR
     SKI