ID   CCL11_HUMLU             Reviewed;         563 AA.
AC   M4ISH2;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Probable CoA ligase CCL11 {ECO:0000305|PubMed:23300257};
DE            Short=HlCCL11 {ECO:0000303|PubMed:23300257};
DE            EC=6.2.1.- {ECO:0000305|PubMed:23300257};
GN   Name=CCL11 {ECO:0000303|PubMed:23300257};
OS   Humulus lupulus (European hop).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Humulus.
OX   NCBI_TaxID=3486;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Nugget;
RX   PubMed=23300257; DOI=10.1093/mp/sst004;
RA   Xu H., Zhang F., Liu B., Huhman D.V., Sumner L.W., Dixon R.A., Wang G.;
RT   "Characterization of the formation of branched short-chain fatty acid:CoAs
RT   for bitter acid biosynthesis in hop glandular trichomes.";
RL   Mol. Plant 6:1301-1317(2013).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:M4IRL4}.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are involved in
CC       the substrate recognition, and are sufficient to confer the substrate
CC       specificity. {ECO:0000250|UniProtKB:Q42524}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; JQ740213; AGA17928.1; -; mRNA.
DR   AlphaFoldDB; M4ISH2; -.
DR   SMR; M4ISH2; -.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016405; F:CoA-ligase activity; ISS:UniProtKB.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN           1..563
FT                   /note="Probable CoA ligase CCL11"
FT                   /id="PRO_0000452956"
FT   REGION          263..328
FT                   /note="SBD1"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   REGION          329..405
FT                   /note="SBD2"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   BINDING         195..203
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         328..333
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         426
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         438..441
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         534
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
SQ   SEQUENCE   563 AA;  62176 MW;  85CFF342005F142C CRC64;
     MEELKPRFAN SCPLTPLGFL ERAATVYGDC ISVVYDDLSY TWSQTHTRCL RVASCIESCL
     GVKKGQVVSV VAPNVPAMYE LNFAVPMAGV VLNNINTRLN AVTISVMLRH SESKLVFVDQ
     LSVRLVLDAV SLFPKNTPTP LLVLIADNIT GENLTVEDRE HFVCCYEDLV EKGDDKTFKW
     VRPISEWDPI VLNYTSGTTS SPKGVVHSHR SVFVVTLDSL IDWGVPKQPV YLWTLPMFHA
     NGWGYTWGIA AVGGTNICLR RFDGEIIFNL IRRHRVTHMC AAPIVLNMLS NSPNAEPLPN
     PVHVMTGGAP PPAAVLLRTE SLGFVISHGY GMTEMLGVVV SCAWKREWNR LPATEQARLK
     SRQGVRTAAM MEVDVVDPNS GVSVKRDGLT MGEIVLKGSS IMLGYLKNSA ATAKCIRADG
     WFYTGDMAVM HPDGYLEIKD RSKDVIISGG ENVSSVEVES ALYSHPAVDE AAVVACPDEY
     WGETPFAFVT LKKGMRVRPT EKEILEYCRE KLAHFMVPKV VVFRDKLPKT STGKIQKFTL
     KEIVKTMGYS SSSSSYGVGR ARM