CCL12_MOUSE
ID CCL12_MOUSE Reviewed; 104 AA.
AC Q62401; Q9QYD6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=C-C motif chemokine 12;
DE AltName: Full=MCP-1-related chemokine;
DE AltName: Full=Monocyte chemoattractant protein 5;
DE AltName: Full=Monocyte chemotactic protein 5;
DE Short=MCP-5;
DE AltName: Full=Small-inducible cytokine A12;
DE Flags: Precursor;
GN Name=Ccl12; Synonyms=Mcp5, Scya12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8920881; DOI=10.1084/jem.184.5.1939;
RA Jia G.-Q., Gonzalo J.A., Lloyd C., Kremer L., Lu L., Martinez A.C.,
RA Wershil B.K., Gutierrez-Ramos J.-C.;
RT "Distinct expression and function of the novel mouse chemokine monocyte
RT chemotactic protein-5 in lung allergic inflammation.";
RL J. Exp. Med. 184:1939-1951(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8996246; DOI=10.1084/jem.185.1.99;
RA Sarafi M.N., Garcia-Zepeda E.A., MacLean J.A., Charo I.F., Luster A.D.;
RT "Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that
RT is a structural and functional homologue of human MCP-1.";
RL J. Exp. Med. 185:99-109(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], POLYMORPHISM, AND VARIANT 94-GLN--GLY-104
RP DELINS ARG-THR.
RC STRAIN=B10.S/J, BALB/cJ, DBA/2J, NOD/LtJ, and SJL/J; TISSUE=Spleen;
RX PubMed=10438970;
RA Teuscher C., Butterfield R.J., Ma R.Z., Zachary J.F., Doerge R.W.,
RA Blankenhorn E.P.;
RT "Sequence polymorphisms in the chemokines Scya1 (TCA-3), Scya2 (monocyte
RT chemoattractant protein (MCP)-1), and Scya12 (MCP-5) are candidates for
RT eae7, a locus controlling susceptibility to monophasic
RT remitting/nonrelapsing experimental allergic encephalomyelitis.";
RL J. Immunol. 163:2262-2266(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Chemotactic factor that attracts eosinophils, monocytes, and
CC lymphocytes but not neutrophils. Potent monocyte active chemokine that
CC signals through CCR2. Involved in allergic inflammation and the host
CC response to pathogens and may play a pivotal role during early stages
CC of allergic lung inflammation.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the lymph nodes and
CC thymus. Also found in the salivary glands containing lymph nodes,
CC breast, heart, lung, brain, small intestine, kidney and colon.
CC -!- INDUCTION: By interferon gamma and lipopolysaccharides (LPS).
CC -!- POLYMORPHISM: The polymorphism in strain SJL/J may be associated with
CC severity of clinical symptoms of experimental allergic
CC encephalomyelitis, an animal model of multiple sclerosis, and
CC susceptibility to the monophasic remitting/nonrelapsing form of the
CC disease. {ECO:0000269|PubMed:10438970}.
CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U50712; AAB50053.1; -; mRNA.
DR EMBL; U66670; AAB49424.1; -; mRNA.
DR EMBL; AF065934; AAF15384.1; -; mRNA.
DR EMBL; AF065935; AAF15385.1; -; mRNA.
DR EMBL; AF065936; AAF15386.1; -; mRNA.
DR EMBL; AF065937; AAF15387.1; -; mRNA.
DR EMBL; AF065938; AAF15388.1; -; mRNA.
DR EMBL; BC027520; AAH27520.1; -; mRNA.
DR CCDS; CCDS36247.1; -.
DR RefSeq; NP_035461.2; NM_011331.2.
DR AlphaFoldDB; Q62401; -.
DR SMR; Q62401; -.
DR STRING; 10090.ENSMUSP00000000194; -.
DR PaxDb; Q62401; -.
DR PeptideAtlas; Q62401; -.
DR PRIDE; Q62401; -.
DR DNASU; 20293; -.
DR Ensembl; ENSMUST00000000194; ENSMUSP00000000194; ENSMUSG00000035352.
DR GeneID; 20293; -.
DR KEGG; mmu:20293; -.
DR UCSC; uc007kms.1; mouse.
DR CTD; 20293; -.
DR MGI; MGI:108224; Ccl12.
DR VEuPathDB; HostDB:ENSMUSG00000035352; -.
DR eggNOG; ENOG502S6ZP; Eukaryota.
DR GeneTree; ENSGT01050000244851; -.
DR HOGENOM; CLU_141716_1_0_1; -.
DR InParanoid; Q62401; -.
DR OMA; PITCCYT; -.
DR OrthoDB; 1575018at2759; -.
DR PhylomeDB; Q62401; -.
DR TreeFam; TF334888; -.
DR Reactome; R-MMU-380108; Chemokine receptors bind chemokines.
DR BioGRID-ORCS; 20293; 0 hits in 60 CRISPR screens.
DR PRO; PR:Q62401; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q62401; protein.
DR Bgee; ENSMUSG00000035352; Expressed in peripheral lymph node and 87 other tissues.
DR ExpressionAtlas; Q62401; baseline and differential.
DR Genevisible; Q62401; MM.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0031727; F:CCR2 chemokine receptor binding; IPI:BHF-UCL.
DR GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR GO; GO:0016004; F:phospholipase activator activity; ISO:MGI.
DR GO; GO:0004672; F:protein kinase activity; ISO:MGI.
DR GO; GO:0001525; P:angiogenesis; IDA:BHF-UCL.
DR GO; GO:0043615; P:astrocyte cell migration; ISO:MGI.
DR GO; GO:0071346; P:cellular response to interferon-gamma; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; ISO:MGI.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; ISO:MGI.
DR GO; GO:0007010; P:cytoskeleton organization; ISO:MGI.
DR GO; GO:0048245; P:eosinophil chemotaxis; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IDA:BHF-UCL.
DR GO; GO:0031663; P:lipopolysaccharide-mediated signaling pathway; ISO:MGI.
DR GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR GO; GO:0048246; P:macrophage chemotaxis; ISO:MGI.
DR GO; GO:0000165; P:MAPK cascade; ISO:MGI.
DR GO; GO:0002548; P:monocyte chemotaxis; IDA:BHF-UCL.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; ISO:MGI.
DR GO; GO:0034351; P:negative regulation of glial cell apoptotic process; ISO:MGI.
DR GO; GO:0070664; P:negative regulation of leukocyte proliferation; ISO:MGI.
DR GO; GO:2000502; P:negative regulation of natural killer cell chemotaxis; ISO:MGI.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISO:MGI.
DR GO; GO:1905563; P:negative regulation of vascular endothelial cell proliferation; ISO:MGI.
DR GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR GO; GO:0090280; P:positive regulation of calcium ion import; ISO:MGI.
DR GO; GO:2000353; P:positive regulation of endothelial cell apoptotic process; ISO:MGI.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0002687; P:positive regulation of leukocyte migration; ISO:MGI.
DR GO; GO:0043491; P:protein kinase B signaling; ISO:MGI.
DR GO; GO:0008360; P:regulation of cell shape; ISO:MGI.
DR InterPro; IPR039809; Chemokine_b/g/d.
DR InterPro; IPR000827; Chemokine_CC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR12015; PTHR12015; 1.
DR Pfam; PF00048; IL8; 1.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
PE 2: Evidence at transcript level;
KW Chemotaxis; Cytokine; Disulfide bond; Inflammatory response;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000250"
FT CHAIN 23..104
FT /note="C-C motif chemokine 12"
FT /id="PRO_0000005199"
FT DISULFID 33..58
FT /evidence="ECO:0000250"
FT DISULFID 34..74
FT /evidence="ECO:0000250"
FT VARIANT 94..104
FT /note="QTFILEPSCLG -> RT (in strain: SJL/J)"
FT /evidence="ECO:0000269|PubMed:10438970"
SQ SEQUENCE 104 AA; 11659 MW; 8D102F4FF4CC3DBF CRC64;
MKISTLLCLL LIATTISPQV LAGPDAVSTP VTCCYNVVKQ KIHVRKLKSY RRITSSQCPR
EAVIFRTILD KEICADPKEK WVKNSINHLD KTSQTFILEP SCLG
