ID   CCL13_HUMLU             Reviewed;         573 AA.
AC   M4IS92;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Probable CoA ligase CCL13 {ECO:0000305|PubMed:23300257};
DE            Short=HlCCL13 {ECO:0000303|PubMed:23300257};
DE            EC=6.2.1.- {ECO:0000305|PubMed:23300257};
GN   Name=CCL13 {ECO:0000303|PubMed:23300257};
OS   Humulus lupulus (European hop).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Humulus.
OX   NCBI_TaxID=3486;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Nugget;
RX   PubMed=23300257; DOI=10.1093/mp/sst004;
RA   Xu H., Zhang F., Liu B., Huhman D.V., Sumner L.W., Dixon R.A., Wang G.;
RT   "Characterization of the formation of branched short-chain fatty acid:CoAs
RT   for bitter acid biosynthesis in hop glandular trichomes.";
RL   Mol. Plant 6:1301-1317(2013).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:M4IRL4}.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are involved in
CC       the substrate recognition, and are sufficient to confer the substrate
CC       specificity. {ECO:0000250|UniProtKB:Q42524}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; JQ740215; AGA17930.1; -; mRNA.
DR   AlphaFoldDB; M4IS92; -.
DR   SMR; M4IS92; -.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016405; F:CoA-ligase activity; ISS:UniProtKB.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN           1..573
FT                   /note="Probable CoA ligase CCL13"
FT                   /id="PRO_0000452958"
FT   REGION          284..352
FT                   /note="SBD1"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   REGION          353..429
FT                   /note="SBD2"
FT                   /evidence="ECO:0000250|UniProtKB:Q42524"
FT   BINDING         216..224
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         352..357
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         449
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         461..464
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
FT   BINDING         556
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:Q81G39"
SQ   SEQUENCE   573 AA;  63221 MW;  F382ED039585DBC7 CRC64;
     MDNYRRLHTP VALCVASPPA PPTTSWKSME GLVQCSANHV PLSPITFLER SSKAYRDNTS
     LVYGSVRYTW AQTHHRCLKL ASALTTHFGI SPGDVVATFS YNIPEIYELH FAVPMAGGIL
     CTLNARNDSA MVSTLLAHSE AKLIFVEPQL LETARAALDL LAQKDIKPPT LVLLTDSESF
     TSSSYDHYNH LLANGSDDFE IRRPKNECDP ISINYTSGTT ARPKAVVYSH RGAYLNSIAT
     VLLHGMGTRS VYLWSVPMFH CNGWCFPWGA AAQGATNICI RKVSPKAIFD NIHLHKVTHF
     GAAPTVLNMI VNSPEGNLHT PLPHKVEVMT GGSPPPPKVI ARMEEMGFQV NHIYGLTETH
     GPATNCVCKP EWDALQPEER YALKARQGLN HLAMEEMDVR DPVSMESVRA DGTTIGEVMF
     RGNTVMSGYF KDLKATEEAF EGGWFRTGDL GVKHEDGYIQ LKDRKKDVVI SGGENVSTVE
     VETVLYSHEA VLEAAVVARP DKLWGETPCA FVTLKEGFDN GVSADQIIKF CRDRLPHYMA
     PKTVVFEELP KTSTGKIQKY ILKEKAKAMG SLS