CCL25_HUMAN
ID CCL25_HUMAN Reviewed; 150 AA.
AC O15444; A1L4J4; A6NI52; A8K9E7; B5MCA5; Q96KJ7;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=C-C motif chemokine 25;
DE AltName: Full=Chemokine TECK;
DE AltName: Full=Small-inducible cytokine A25;
DE AltName: Full=Thymus-expressed chemokine;
DE Flags: Precursor;
GN Name=CCL25; Synonyms=SCYA25, TECK;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANT MET-104.
RC TISSUE=Thymus;
RX PubMed=9285413; DOI=10.1016/s1074-7613(00)80531-2;
RA Vicari A.P., Figueroa D.J., Hedrick J.A., Foster J.S., Singh K.P.,
RA Menon S., Copeland N.G., Gilbert D.J., Jenkins N.A., Bacon K.B.,
RA Zlotnik A.;
RT "TECK: a novel CC chemokine specifically expressed by thymic dendritic
RT cells and potentially involved in T cell development.";
RL Immunity 7:291-301(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Thymus;
RA Hieshima K., Nakayama T., Fujisawa R., Izawa D., Yoshie O.;
RT "Molecular cloning and characterization of a splicing variant of chemokine
RT TECK: a natural antagonist of human TECK.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP RECEPTOR INTERACTION.
RX PubMed=10229797;
RA Zaballos A., Gutierrez J., Varona R., Ardavin C., Marquez G.;
RT "Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the
RT receptor for the chemokine TECK.";
RL J. Immunol. 162:5671-5675(1999).
RN [8]
RP RECEPTOR INTERACTION.
RX PubMed=23341447; DOI=10.1074/jbc.m112.406108;
RA Watts A.O., Verkaar F., van der Lee M.M., Timmerman C.A., Kuijer M.,
RA van Offenbeek J., van Lith L.H., Smit M.J., Leurs R., Zaman G.J.,
RA Vischer H.F.;
RT "Beta-arrestin recruitment and G protein signaling by the atypical human
RT chemokine decoy receptor CCX-CKR.";
RL J. Biol. Chem. 288:7169-7181(2013).
CC -!- FUNCTION: Potentially involved in T-cell development. Recombinant
CC protein shows chemotactic activity on thymocytes, macrophages, THP-1
CC cells, and dendritics cells but is inactive on peripheral blood
CC lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist
CC of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates
CC the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
CC -!- INTERACTION:
CC O15444; Q92583: CCL17; NbExp=2; IntAct=EBI-7783341, EBI-16640146;
CC O15444; P13501: CCL5; NbExp=2; IntAct=EBI-7783341, EBI-2848366;
CC O15444; P48061: CXCL12; NbExp=2; IntAct=EBI-7783341, EBI-3913254;
CC O15444; P02776: PF4; NbExp=3; IntAct=EBI-7783341, EBI-2565740;
CC O15444; P10720: PF4V1; NbExp=2; IntAct=EBI-7783341, EBI-1223944;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O15444-1; Sequence=Displayed;
CC Name=2; Synonyms=TECKvar;
CC IsoId=O15444-2; Sequence=VSP_001064;
CC Name=3;
CC IsoId=O15444-3; Sequence=VSP_043199;
CC -!- TISSUE SPECIFICITY: Specifically expressed by thymic dendritic cells.
CC High levels in thymus and small intestine.
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Wikipedia; Note=CCL25 entry;
CC URL="https://en.wikipedia.org/wiki/CCL25";
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DR EMBL; U86358; AAB69981.1; -; mRNA.
DR EMBL; AB046579; BAB62257.1; -; mRNA.
DR EMBL; AK292662; BAF85351.1; -; mRNA.
DR EMBL; AC008946; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471139; EAW68952.1; -; Genomic_DNA.
DR EMBL; BC130561; AAI30562.1; -; mRNA.
DR EMBL; BC144463; AAI44464.1; -; mRNA.
DR CCDS; CCDS12194.1; -. [O15444-1]
DR CCDS; CCDS56080.1; -. [O15444-3]
DR RefSeq; NP_001188288.1; NM_001201359.1. [O15444-3]
DR RefSeq; NP_005615.2; NM_005624.3. [O15444-1]
DR RefSeq; XP_011526479.1; XM_011528177.2. [O15444-1]
DR RefSeq; XP_016882609.1; XM_017027120.1. [O15444-1]
DR RefSeq; XP_016882610.1; XM_017027121.1. [O15444-1]
DR RefSeq; XP_016882611.1; XM_017027122.1. [O15444-3]
DR AlphaFoldDB; O15444; -.
DR SMR; O15444; -.
DR BioGRID; 112273; 10.
DR DIP; DIP-5883N; -.
DR IntAct; O15444; 11.
DR MINT; O15444; -.
DR STRING; 9606.ENSP00000375086; -.
DR iPTMnet; O15444; -.
DR PhosphoSitePlus; O15444; -.
DR BioMuta; CCL25; -.
DR MassIVE; O15444; -.
DR PaxDb; O15444; -.
DR PeptideAtlas; O15444; -.
DR PRIDE; O15444; -.
DR ProteomicsDB; 48672; -. [O15444-1]
DR ProteomicsDB; 48673; -. [O15444-2]
DR ProteomicsDB; 48674; -. [O15444-3]
DR Antibodypedia; 24738; 402 antibodies from 30 providers.
DR DNASU; 6370; -.
DR Ensembl; ENST00000253451.9; ENSP00000253451.4; ENSG00000131142.14. [O15444-3]
DR Ensembl; ENST00000315626.6; ENSP00000324756.6; ENSG00000131142.14. [O15444-1]
DR Ensembl; ENST00000390669.7; ENSP00000375086.3; ENSG00000131142.14. [O15444-1]
DR Ensembl; ENST00000680450.1; ENSP00000506202.1; ENSG00000131142.14. [O15444-3]
DR Ensembl; ENST00000680506.1; ENSP00000505422.1; ENSG00000131142.14. [O15444-1]
DR Ensembl; ENST00000680646.1; ENSP00000504875.1; ENSG00000131142.14. [O15444-1]
DR Ensembl; ENST00000681526.1; ENSP00000505387.1; ENSG00000131142.14. [O15444-3]
DR GeneID; 6370; -.
DR KEGG; hsa:6370; -.
DR MANE-Select; ENST00000315626.6; ENSP00000324756.6; NM_005624.4; NP_005615.2.
DR UCSC; uc002mjc.5; human. [O15444-1]
DR CTD; 6370; -.
DR DisGeNET; 6370; -.
DR GeneCards; CCL25; -.
DR HGNC; HGNC:10624; CCL25.
DR HPA; ENSG00000131142; Group enriched (intestine, lymphoid tissue).
DR MIM; 602565; gene.
DR neXtProt; NX_O15444; -.
DR OpenTargets; ENSG00000131142; -.
DR PharmGKB; PA35556; -.
DR VEuPathDB; HostDB:ENSG00000131142; -.
DR eggNOG; ENOG502S8D1; Eukaryota.
DR GeneTree; ENSGT01050000244920; -.
DR HOGENOM; CLU_113773_0_0_1; -.
DR InParanoid; O15444; -.
DR OMA; WAPAVHA; -.
DR OrthoDB; 1542802at2759; -.
DR PhylomeDB; O15444; -.
DR TreeFam; TF353160; -.
DR PathwayCommons; O15444; -.
DR Reactome; R-HSA-380108; Chemokine receptors bind chemokines.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; O15444; -.
DR SIGNOR; O15444; -.
DR BioGRID-ORCS; 6370; 11 hits in 1065 CRISPR screens.
DR GenomeRNAi; 6370; -.
DR Pharos; O15444; Tbio.
DR PRO; PR:O15444; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; O15444; protein.
DR Bgee; ENSG00000131142; Expressed in duodenum and 75 other tissues.
DR ExpressionAtlas; O15444; baseline and differential.
DR Genevisible; O15444; HS.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0031735; F:CCR10 chemokine receptor binding; IDA:BHF-UCL.
DR GO; GO:0008009; F:chemokine activity; IDA:UniProtKB.
DR GO; GO:0042379; F:chemokine receptor binding; IDA:UniProtKB.
DR GO; GO:0005179; F:hormone activity; TAS:ProtInc.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IDA:UniProtKB.
DR GO; GO:0060326; P:cell chemotaxis; IDA:UniProtKB.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0071346; P:cellular response to interferon-gamma; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006935; P:chemotaxis; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:UniProtKB.
DR GO; GO:0006955; P:immune response; TAS:ProtInc.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
DR GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR GO; GO:0002548; P:monocyte chemotaxis; IBA:GO_Central.
DR GO; GO:1903237; P:negative regulation of leukocyte tethering or rolling; IDA:UniProtKB.
DR GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR GO; GO:0001954; P:positive regulation of cell-matrix adhesion; IDA:UniProtKB.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR CDD; cd01119; Chemokine_CC_DCCL; 1.
DR InterPro; IPR039809; Chemokine_b/g/d.
DR InterPro; IPR034133; Chemokine_CC_DCCL.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR12015; PTHR12015; 1.
DR Pfam; PF00048; IL8; 1.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chemotaxis; Cytokine; Disulfide bond;
KW Inflammatory response; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..150
FT /note="C-C motif chemokine 25"
FT /id="PRO_0000005235"
FT DISULFID 30..58
FT /evidence="ECO:0000250"
FT DISULFID 31..75
FT /evidence="ECO:0000250"
FT VAR_SEQ 65..150
FT /note="FYLPKRHRKVCGNPKSREVQRAMKLLDARNKVFAKLHHNTQTFQAGPHAVKK
FT LSSGNSKLSSSKFSNPISSSKRNVSLLISANSGL -> RPSCCKEVEFWKLQVIIIQV
FT (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_001064"
FT VAR_SEQ 109
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_043199"
FT VARIANT 23
FT /note="T -> A (in dbSNP:rs960173)"
FT /id="VAR_044519"
FT VARIANT 101
FT /note="H -> R (in dbSNP:rs2032887)"
FT /id="VAR_044520"
FT VARIANT 104
FT /note="T -> M (in dbSNP:rs1129763)"
FT /evidence="ECO:0000269|PubMed:9285413"
FT /id="VAR_044521"
SQ SEQUENCE 150 AA; 16609 MW; 91D0810F137FBC7B CRC64;
MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL
PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL HHNTQTFQAG PHAVKKLSSG
NSKLSSSKFS NPISSSKRNV SLLISANSGL
