CCL2_BOVIN
ID CCL2_BOVIN Reviewed; 99 AA.
AC P28291; A9QWP7;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=C-C motif chemokine 2;
DE AltName: Full=Acidic seminal fluid protein;
DE AltName: Full=Monocyte chemotactic protein 1A;
DE Short=MCP-1;
DE Short=MCP-1A;
DE AltName: Full=Small-inducible cytokine A2;
DE Flags: Precursor;
GN Name=CCL2; Synonyms=SCYA2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seminal plasma;
RX PubMed=1721821; DOI=10.1089/dna.1991.10.671;
RA Wempe F., Henschen A., Scheit K.H.;
RT "Gene expression and cDNA cloning identified a major basic protein
RT constituent of bovine seminal plasma as bovine monocyte-chemoattractant
RT protein-1 (MCP-1).";
RL DNA Cell Biol. 10:671-679(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seminal plasma;
RX PubMed=1543494; DOI=10.1016/0006-291x(92)91633-2;
RA Wempe F., Einspanier R., Scheit K.H.;
RT "Characterization by cDNA cloning of the mRNA of a new growth factor from
RT bovine seminal plasma: acidic seminal fluid protein.";
RL Biochem. Biophys. Res. Commun. 183:232-237(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8060303; DOI=10.1006/bbrc.1994.2068;
RA Wempe F., Kuhlmann J.K., Scheit K.H.;
RT "Characterization of the bovine monocyte chemoattractant protein-1 gene.";
RL Biochem. Biophys. Res. Commun. 202:1272-1279(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Belted Galloway; TISSUE=Peripheral blood;
RG U.S. Veterinary Immune Reagent Network;
RA Hudgens T., Tompkins D., Baldwin C.L.;
RT "U.S. veterinary immune reagent network: expressed bovine gene sequences.";
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as a ligand for C-C chemokine receptor CCR2 (By
CC similarity). Signals through binding and activation of CCR2 and induces
CC a strong chemotactic response and mobilization of intracellular calcium
CC ions (By similarity). Exhibits a chemotactic activity for monocytes and
CC basophils but not neutrophils or eosinophils (By similarity). Plays an
CC important role in mediating peripheral nerve injury-induced neuropathic
CC pain (By similarity). Increases NMDA-mediated synaptic transmission in
CC both dopamine D1 and D2 receptor-containing neurons, which may be
CC caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By
CC similarity). {ECO:0000250|UniProtKB:P10148,
CC ECO:0000250|UniProtKB:P13500}.
CC -!- SUBUNIT: Monomer or homodimer; in equilibrium. Is tethered on
CC endothelial cells by glycosaminoglycan (GAG) side chains of
CC proteoglycans. Interacts with TNFAIP6 (via Link domain).
CC {ECO:0000250|UniProtKB:P13500}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P13500}.
CC -!- PTM: Processing at the N-terminus can regulate receptor and target cell
CC selectivity (By similarity). Deletion of the N-terminal residue
CC converts it from an activator of basophil to an eosinophil
CC chemoattractant (By similarity). {ECO:0000250|UniProtKB:P13500}.
CC -!- PTM: N-Glycosylated. {ECO:0000250|UniProtKB:P13500}.
CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC {ECO:0000305}.
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DR EMBL; M84602; AAA30651.1; -; mRNA.
DR EMBL; L32659; AAA60956.1; -; Genomic_DNA.
DR EMBL; EU276059; ABX72057.1; -; mRNA.
DR PIR; A39296; A39296.
DR RefSeq; NP_776431.1; NM_174006.2.
DR AlphaFoldDB; P28291; -.
DR SMR; P28291; -.
DR STRING; 9913.ENSBTAP00000013146; -.
DR PaxDb; P28291; -.
DR Ensembl; ENSBTAT00000013146; ENSBTAP00000013146; ENSBTAG00000037811.
DR GeneID; 281043; -.
DR KEGG; bta:281043; -.
DR CTD; 6347; -.
DR VEuPathDB; HostDB:ENSBTAG00000037811; -.
DR eggNOG; ENOG502S6ZP; Eukaryota.
DR GeneTree; ENSGT01050000244851; -.
DR HOGENOM; CLU_141716_1_0_1; -.
DR InParanoid; P28291; -.
DR OMA; PITCCYT; -.
DR OrthoDB; 1575018at2759; -.
DR TreeFam; TF334888; -.
DR Reactome; R-BTA-380108; Chemokine receptors bind chemokines.
DR Proteomes; UP000009136; Chromosome 19.
DR Bgee; ENSBTAG00000037811; Expressed in intramuscular adipose tissue and 98 other tissues.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR GO; GO:0043615; P:astrocyte cell migration; IEA:Ensembl.
DR GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IEA:Ensembl.
DR GO; GO:0071346; P:cellular response to interferon-gamma; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0007010; P:cytoskeleton organization; IEA:Ensembl.
DR GO; GO:0048245; P:eosinophil chemotaxis; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Ensembl.
DR GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR GO; GO:0048246; P:macrophage chemotaxis; IEA:Ensembl.
DR GO; GO:0000165; P:MAPK cascade; IEA:Ensembl.
DR GO; GO:0002548; P:monocyte chemotaxis; IBA:GO_Central.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IEA:Ensembl.
DR GO; GO:0034351; P:negative regulation of glial cell apoptotic process; IEA:Ensembl.
DR GO; GO:2000502; P:negative regulation of natural killer cell chemotaxis; IEA:Ensembl.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Ensembl.
DR GO; GO:1905563; P:negative regulation of vascular endothelial cell proliferation; IEA:Ensembl.
DR GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR GO; GO:0090280; P:positive regulation of calcium ion import; IEA:Ensembl.
DR GO; GO:2000353; P:positive regulation of endothelial cell apoptotic process; IEA:Ensembl.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; ISS:UniProtKB.
DR GO; GO:0043491; P:protein kinase B signaling; IEA:Ensembl.
DR GO; GO:0008360; P:regulation of cell shape; IEA:Ensembl.
DR GO; GO:0019233; P:sensory perception of pain; ISS:UniProtKB.
DR InterPro; IPR039809; Chemokine_b/g/d.
DR InterPro; IPR000827; Chemokine_CC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR12015; PTHR12015; 1.
DR Pfam; PF00048; IL8; 1.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
PE 3: Inferred from homology;
KW Chemotaxis; Cytokine; Disulfide bond; Inflammatory response;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000250"
FT CHAIN 24..99
FT /note="C-C motif chemokine 2"
FT /id="PRO_0000005244"
FT MOD_RES 24
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000250|UniProtKB:P13500"
FT DISULFID 34..59
FT /evidence="ECO:0000250"
FT DISULFID 35..75
FT /evidence="ECO:0000250"
SQ SEQUENCE 99 AA; 11114 MW; 0FD79FC1AB0CBE88 CRC64;
MKVSAALLCL LLTVAAFSTE VLAQPDAINS QVACCYTFNS KKISMQRLMN YRRVTSSKCP
KEAVIFKTIL GKELCADPKQ KWVQDSINYL NKKNQTPKP
