CCL2_HORSE
ID CCL2_HORSE Reviewed; 99 AA.
AC Q9TTQ3;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=C-C motif chemokine 2;
DE AltName: Full=Monocyte chemoattractant protein 1;
DE AltName: Full=Monocyte chemotactic protein 1;
DE Short=MCP-1;
DE AltName: Full=Small-inducible cytokine A2;
DE Flags: Precursor;
GN Name=CCL2; Synonyms=MCP1, SCYA2;
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lung;
RX PubMed=11044560; DOI=10.1016/s0165-2427(00)00222-1;
RA Benarafa C., Cunningham F.M., Hamblin A.S., Horohov D.W., Collins M.E.;
RT "Cloning of equine chemokines eotaxin, monocyte chemoattractant protein
RT (MCP)-1, MCP-2 and MCP-4, mRNA expression in tissues and induction by IL-4
RT in dermal fibroblasts.";
RL Vet. Immunol. Immunopathol. 76:283-298(2000).
CC -!- FUNCTION: Acts as a ligand for C-C chemokine receptor CCR2 (By
CC similarity). Signals through binding and activation of CCR2 and induces
CC a strong chemotactic response and mobilization of intracellular calcium
CC ions (By similarity). Exhibits a chemotactic activity for monocytes and
CC basophils but not neutrophils or eosinophils (By similarity). Plays an
CC important role in mediating peripheral nerve injury-induced neuropathic
CC pain (By similarity). Increases NMDA-mediated synaptic transmission in
CC both dopamine D1 and D2 receptor-containing neurons, which may be
CC caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By
CC similarity). {ECO:0000250|UniProtKB:P10148,
CC ECO:0000250|UniProtKB:P13500}.
CC -!- SUBUNIT: Monomer or homodimer; in equilibrium. Is tethered on
CC endothelial cells by glycosaminoglycan (GAG) side chains of
CC proteoglycans. Interacts with TNFAIP6 (via Link domain).
CC {ECO:0000250|UniProtKB:P13500}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P13500}.
CC -!- PTM: Processing at the N-terminus can regulate receptor and target cell
CC selectivity (By similarity). Deletion of the N-terminal residue
CC converts it from an activator of basophil to an eosinophil
CC chemoattractant (By similarity). {ECO:0000250|UniProtKB:P13500}.
CC -!- PTM: N-Glycosylated. {ECO:0000250|UniProtKB:P13500}.
CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC {ECO:0000305}.
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DR EMBL; AJ251189; CAB61625.1; -; mRNA.
DR RefSeq; NP_001075400.1; NM_001081931.2.
DR AlphaFoldDB; Q9TTQ3; -.
DR SMR; Q9TTQ3; -.
DR STRING; 9796.ENSECAP00000021376; -.
DR PRIDE; Q9TTQ3; -.
DR Ensembl; ENSECAT00000025681; ENSECAP00000021376; ENSECAG00000023949.
DR GeneID; 100034136; -.
DR KEGG; ecb:100034136; -.
DR CTD; 6347; -.
DR GeneTree; ENSGT01050000244851; -.
DR HOGENOM; CLU_141716_1_0_1; -.
DR InParanoid; Q9TTQ3; -.
DR OrthoDB; 1575018at2759; -.
DR Proteomes; UP000002281; Chromosome 11.
DR Bgee; ENSECAG00000023949; Expressed in bone marrow and 18 other tissues.
DR ExpressionAtlas; Q9TTQ3; baseline.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0008009; F:chemokine activity; IBA:GO_Central.
DR GO; GO:0043615; P:astrocyte cell migration; IEA:Ensembl.
DR GO; GO:0044344; P:cellular response to fibroblast growth factor stimulus; IEA:Ensembl.
DR GO; GO:0071346; P:cellular response to interferon-gamma; IBA:GO_Central.
DR GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0007010; P:cytoskeleton organization; IEA:Ensembl.
DR GO; GO:0048245; P:eosinophil chemotaxis; IBA:GO_Central.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Ensembl.
DR GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR GO; GO:0048246; P:macrophage chemotaxis; IEA:Ensembl.
DR GO; GO:0000165; P:MAPK cascade; IEA:Ensembl.
DR GO; GO:0002548; P:monocyte chemotaxis; IBA:GO_Central.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IEA:Ensembl.
DR GO; GO:0034351; P:negative regulation of glial cell apoptotic process; IEA:Ensembl.
DR GO; GO:2000502; P:negative regulation of natural killer cell chemotaxis; IEA:Ensembl.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Ensembl.
DR GO; GO:1905563; P:negative regulation of vascular endothelial cell proliferation; IEA:Ensembl.
DR GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR GO; GO:0090280; P:positive regulation of calcium ion import; IEA:Ensembl.
DR GO; GO:2000353; P:positive regulation of endothelial cell apoptotic process; IEA:Ensembl.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; ISS:UniProtKB.
DR GO; GO:0043491; P:protein kinase B signaling; IEA:Ensembl.
DR GO; GO:0008360; P:regulation of cell shape; IEA:Ensembl.
DR GO; GO:0019233; P:sensory perception of pain; ISS:UniProtKB.
DR InterPro; IPR039809; Chemokine_b/g/d.
DR InterPro; IPR000827; Chemokine_CC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR12015; PTHR12015; 1.
DR Pfam; PF00048; IL8; 1.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
PE 3: Inferred from homology;
KW Chemotaxis; Cytokine; Disulfide bond; Inflammatory response;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..99
FT /note="C-C motif chemokine 2"
FT /id="PRO_0000005145"
FT MOD_RES 24
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000250|UniProtKB:P13500"
FT DISULFID 34..59
FT /evidence="ECO:0000250"
FT DISULFID 35..75
FT /evidence="ECO:0000250"
SQ SEQUENCE 99 AA; 10790 MW; BA1448F79F3423D2 CRC64;
MKVSAALLCL LLTAAAFSTQ VLAQPDAINS PVTCCYTFTG KKISSQRLGS YKRVTSSKCP
KEAVIFKTIL AKEICADPEQ KWVQDAVKQL DKKAQTPKP
