CCL3_RAT
ID CCL3_RAT Reviewed; 92 AA.
AC P50229; A1EC87;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=C-C motif chemokine 3;
DE AltName: Full=Macrophage inflammatory protein 1-alpha;
DE Short=MIP-1-alpha;
DE AltName: Full=Small-inducible cytokine A3;
DE Flags: Precursor;
GN Name=Ccl3; Synonyms=Mip1a, Scya3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lung;
RX PubMed=7779098; DOI=10.1006/bbrc.1995.1809;
RA Shi M.M., Godleski J.J., Paulauskis J.D.;
RT "Molecular cloning and posttranscriptional regulation of macrophage
RT inflammatory protein-1 alpha in alveolar macrophages.";
RL Biochem. Biophys. Res. Commun. 211:289-295(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Long Evans; TISSUE=Lung;
RX PubMed=7722328;
RA Shanley T.P., Schmal H., Friedl H.P., Jones M.L., Ward P.A.;
RT "Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute
RT lung injury in rats.";
RL J. Immunol. 154:4793-4802(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Dahl salt-sensitive, and Lewis;
RX PubMed=17218081; DOI=10.1016/j.ygeno.2006.12.005;
RA Saad Y., Garrett M.R., Manickavasagam E., Yerga-Woolwine S., Farms P.,
RA Radecki T., Joe B.;
RT "Fine-mapping and comprehensive transcript analysis reveals nonsynonymous
RT variants within a novel 1.17 Mb blood pressure QTL region on rat chromosome
RT 10.";
RL Genomics 89:343-353(2007).
RN [4]
RP PROTEIN SEQUENCE OF 24-57.
RC STRAIN=Wistar;
RX PubMed=8607872; DOI=10.1006/bbrc.1996.0511;
RA Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.;
RT "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member
RT of rat GRO/CINCs, is a predominant chemokine produced by
RT lipopolysaccharide-stimulated rat macrophages in culture.";
RL Biochem. Biophys. Res. Commun. 220:945-948(1996).
CC -!- FUNCTION: Monokine with inflammatory and chemokinetic properties. Has
CC chemotactic activity for monocytes, neutrophils, eosinophils,
CC basophils, and lymphocytes. Required for lung TNF-alpha production,
CC neutrophil recruitment and subsequent lung injury and may function as
CC an autocrine mediator for the macrophage production of TNF-alpha which
CC in turn up-regulates vascular adhesion molecules required for
CC neutrophil influx. This protein binds heparin.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- INDUCTION: By lipopolysaccharide (LPS).
CC -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC {ECO:0000305}.
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DR EMBL; U22414; AAA80608.1; -; mRNA.
DR EMBL; U06435; AAA96498.1; -; mRNA.
DR EMBL; EF121994; ABL63433.1; -; mRNA.
DR EMBL; EF121995; ABL63434.1; -; mRNA.
DR PIR; I52322; I52322.
DR RefSeq; NP_037157.2; NM_013025.2.
DR AlphaFoldDB; P50229; -.
DR SMR; P50229; -.
DR STRING; 10116.ENSRNOP00000015139; -.
DR PaxDb; P50229; -.
DR PRIDE; P50229; -.
DR Ensembl; ENSRNOT00000015139; ENSRNOP00000015139; ENSRNOG00000011205.
DR GeneID; 25542; -.
DR KEGG; rno:25542; -.
DR CTD; 6348; -.
DR RGD; 3647; Ccl3.
DR eggNOG; ENOG502SAF0; Eukaryota.
DR GeneTree; ENSGT01050000244851; -.
DR HOGENOM; CLU_141716_4_0_1; -.
DR InParanoid; P50229; -.
DR OMA; MKTSQRC; -.
DR OrthoDB; 1575018at2759; -.
DR PhylomeDB; P50229; -.
DR TreeFam; TF334888; -.
DR Reactome; R-RNO-380108; Chemokine receptors bind chemokines.
DR PRO; PR:P50229; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000011205; Expressed in thymus and 15 other tissues.
DR Genevisible; P50229; RN.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0004698; F:calcium-dependent protein kinase C activity; ISS:UniProtKB.
DR GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0031726; F:CCR1 chemokine receptor binding; ISO:RGD.
DR GO; GO:0031730; F:CCR5 chemokine receptor binding; ISO:RGD.
DR GO; GO:0042056; F:chemoattractant activity; ISS:UniProtKB.
DR GO; GO:0008009; F:chemokine activity; IDA:RGD.
DR GO; GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0016301; F:kinase activity; ISS:UniProtKB.
DR GO; GO:0016004; F:phospholipase activator activity; ISS:UniProtKB.
DR GO; GO:0004672; F:protein kinase activity; ISS:UniProtKB.
DR GO; GO:0043615; P:astrocyte cell migration; ISS:UniProtKB.
DR GO; GO:0006816; P:calcium ion transport; ISS:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0001775; P:cell activation; ISS:UniProtKB.
DR GO; GO:0007267; P:cell-cell signaling; ISS:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISS:UniProtKB.
DR GO; GO:0071346; P:cellular response to interferon-gamma; ISO:RGD.
DR GO; GO:0071347; P:cellular response to interleukin-1; ISO:RGD.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; ISS:UniProtKB.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; ISO:RGD.
DR GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0006935; P:chemotaxis; ISS:UniProtKB.
DR GO; GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0048245; P:eosinophil chemotaxis; ISS:UniProtKB.
DR GO; GO:0043308; P:eosinophil degranulation; ISS:UniProtKB.
DR GO; GO:0006887; P:exocytosis; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0071621; P:granulocyte chemotaxis; ISS:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IEP:RGD.
DR GO; GO:0030595; P:leukocyte chemotaxis; IDA:RGD.
DR GO; GO:0031663; P:lipopolysaccharide-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0048247; P:lymphocyte chemotaxis; ISS:UniProtKB.
DR GO; GO:0048246; P:macrophage chemotaxis; ISS:UniProtKB.
DR GO; GO:0000165; P:MAPK cascade; ISS:UniProtKB.
DR GO; GO:0002548; P:monocyte chemotaxis; ISS:UniProtKB.
DR GO; GO:0043922; P:negative regulation by host of viral transcription; ISS:UniProtKB.
DR GO; GO:0030502; P:negative regulation of bone mineralization; ISO:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0045671; P:negative regulation of osteoclast differentiation; ISS:UniProtKB.
DR GO; GO:0030593; P:neutrophil chemotaxis; IDA:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; ISS:UniProtKB.
DR GO; GO:0051928; P:positive regulation of calcium ion transport; ISS:UniProtKB.
DR GO; GO:0050850; P:positive regulation of calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0030335; P:positive regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:UniProtKB.
DR GO; GO:0032731; P:positive regulation of interleukin-1 beta production; ISS:UniProtKB.
DR GO; GO:2000503; P:positive regulation of natural killer cell chemotaxis; ISS:UniProtKB.
DR GO; GO:0043525; P:positive regulation of neuron apoptotic process; ISS:UniProtKB.
DR GO; GO:0045672; P:positive regulation of osteoclast differentiation; IDA:RGD.
DR GO; GO:0051897; P:positive regulation of protein kinase B signaling; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0032760; P:positive regulation of tumor necrosis factor production; ISS:UniProtKB.
DR GO; GO:0043491; P:protein kinase B signaling; ISS:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0050795; P:regulation of behavior; ISS:UniProtKB.
DR GO; GO:0008360; P:regulation of cell shape; ISS:UniProtKB.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISS:UniProtKB.
DR GO; GO:0014808; P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0070723; P:response to cholesterol; ISS:UniProtKB.
DR GO; GO:0009636; P:response to toxic substance; ISS:UniProtKB.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0023052; P:signaling; ISS:UniProtKB.
DR GO; GO:0010818; P:T cell chemotaxis; ISS:UniProtKB.
DR InterPro; IPR039809; Chemokine_b/g/d.
DR InterPro; IPR000827; Chemokine_CC_CS.
DR InterPro; IPR001811; Chemokine_IL8-like_dom.
DR InterPro; IPR036048; Interleukin_8-like_sf.
DR PANTHER; PTHR12015; PTHR12015; 1.
DR Pfam; PF00048; IL8; 1.
DR SMART; SM00199; SCY; 1.
DR SUPFAM; SSF54117; SSF54117; 1.
DR PROSITE; PS00472; SMALL_CYTOKINES_CC; 1.
PE 1: Evidence at protein level;
KW Chemotaxis; Cytokine; Direct protein sequencing; Disulfide bond;
KW Heparin-binding; Inflammatory response; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:8607872"
FT CHAIN 24..92
FT /note="C-C motif chemokine 3"
FT /id="PRO_0000005160"
FT DISULFID 34..57
FT /evidence="ECO:0000250|UniProtKB:P10147"
FT DISULFID 35..73
FT /evidence="ECO:0000250|UniProtKB:P10147"
FT CONFLICT 6
FT /note="A -> T (in Ref. 2; AAA96498)"
FT /evidence="ECO:0000305"
FT CONFLICT 57
FT /note="C -> W (in Ref. 2; AAA96498 and 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 92 AA; 10335 MW; 14E861C647F9A2EB CRC64;
MKVSTAALAV LLCTMALWNE VFSAPYGADT PTACCFSYGR QIPRKFIADY FETSSLCSQP
GVIFLTKRNR QICADPKETW VQEYITELEL NA
