ID   CCLOP_LOTJA             Reviewed;         518 AA.
AC   A9XMT3;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Protein CYCLOPS {ECO:0000303|PubMed:19074278};
DE            Short=LjCYCLOPS {ECO:0000303|PubMed:19074278};
DE   AltName: Full=Protein IPD3 homolog {ECO:0000305};
GN   Name=IPD3 {ECO:0000305};
OS   Lotus japonicus (Lotus corniculatus var. japonicus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, HOMODIMERIZATION, INTERACTION WITH
RP   CCAMK, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY RHIZOBIA,
RP   PHOSPHORYLATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=19074278; DOI=10.1073/pnas.0806858105;
RA   Yano K., Yoshida S., Mueller J., Singh S., Banba M., Vickers K.,
RA   Markmann K., White C., Schuller B., Sato S., Asamizu E., Tabata S.,
RA   Murooka Y., Perry J., Wang T.L., Kawaguchi M., Imaizumi-Anraku H.,
RA   Hayashi M., Parniske M.;
RT   "CYCLOPS, a mediator of symbiotic intracellular accommodation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:20540-20545(2008).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=cv. Gifu;
RX   PubMed=27020747; DOI=10.1016/j.cub.2016.01.069;
RA   Pimprikar P., Carbonnel S., Paries M., Katzer K., Klingl V., Bohmer M.J.,
RA   Karl L., Floss D.S., Harrison M.J., Parniske M., Gutjahr C.;
RT   "A CCaMK-CYCLOPS-DELLA complex activates transcription of RAM1 to regulate
RT   arbuscule branching.";
RL   Curr. Biol. 26:987-998(2016).
RN   [3]
RP   FUNCTION.
RX   PubMed=28503742; DOI=10.1111/nph.14547;
RA   Cerri M.R., Wang Q., Stolz P., Folgmann J., Frances L., Katzer K., Li X.,
RA   Heckmann A.B., Wang T.L., Downie J.A., Klingl A., de Carvalho-Niebel F.,
RA   Xie F., Parniske M.;
RT   "The ERN1 transcription factor gene is a target of the CCaMK/CYCLOPS
RT   complex and controls rhizobial infection in Lotus japonicus.";
RL   New Phytol. 215:323-337(2017).
CC   -!- FUNCTION: Involved in symbiotic signaling. Required for root infection
CC       by symbiotic rhizobia, infection thread (IT) formation, and nodule
CC       development (PubMed:19074278). Probably not involved in nodule
CC       organogenesis (PubMed:19074278). Involved in arbuscular mycorrhizal
CC       (AM) symbiosis (PubMed:19074278). Required for fungal infection of the
CC       outer cortical cell layers, and for arbuscule development during the AM
CC       symbiosis, by binding, as a complex comprising CCaMK, CYCLOPS, and
CC       DELLA, to RAM1 promoter cis element thus promoting its expression
CC       (PubMed:19074278, PubMed:27020747). Acts downstream of CCAMK
CC       (PubMed:19074278). Binds to the promoter of ERN1 and strongly
CC       transactivates ERN1, a transcriptional regulator required for
CC       nodulation (PubMed:28503742). {ECO:0000269|PubMed:19074278,
CC       ECO:0000269|PubMed:27020747, ECO:0000269|PubMed:28503742}.
CC   -!- SUBUNIT: Forms homodimers. Interacts with CCAMK.
CC       {ECO:0000269|PubMed:19074278}.
CC   -!- INTERACTION:
CC       A9XMT3; A0AAR7: CCAMK; NbExp=4; IntAct=EBI-15746220, EBI-15746225;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19074278}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:19074278}.
CC   -!- INDUCTION: Induced in nodules 21 days after rhizobial inoculation.
CC       {ECO:0000269|PubMed:19074278}.
CC   -!- PTM: Phosphorylated at the N-terminus by CCAMK.
CC       {ECO:0000269|PubMed:19074278}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions, but roots of mutant plants are impaired in the interaction
CC       with both rhizobia and the arbuscular mycorrhiza (AM) fungus Glomus
CC       intraradices (PubMed:19074278, PubMed:27020747). Stunted growth when
CC       grown in nitrogen-limiting conditions and in presence of Mesorhizobium
CC       loti (PubMed:19074278). {ECO:0000269|PubMed:19074278,
CC       ECO:0000269|PubMed:27020747}.
CC   -!- MISCELLANEOUS: Hairy roots of ipd3 mutant plants expressing a
CC       constitutively active form of CCAMK exhibit spontaneous nodule
CC       formation in the absence of rhizobia. {ECO:0000269|PubMed:19074278}.
CC   -!- SIMILARITY: Belongs to the CYCLOPS family. {ECO:0000305}.
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DR   EMBL; EF569221; ABU63668.1; -; mRNA.
DR   AlphaFoldDB; A9XMT3; -.
DR   SMR; A9XMT3; -.
DR   DIP; DIP-48654N; -.
DR   IntAct; A9XMT3; 1.
DR   OMA; FLAKAWF; -.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR   GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR   InterPro; IPR040036; CYCLOPS.
DR   PANTHER; PTHR36890; PTHR36890; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Nodulation; Nucleus.
FT   CHAIN           1..518
FT                   /note="Protein CYCLOPS"
FT                   /id="PRO_0000444702"
FT   REGION          401..451
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          452..518
FT                   /evidence="ECO:0000255"
FT   MOTIF           402..405
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   MOTIF           426..429
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        420..441
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   518 AA;  58037 MW;  AE3C47891B3DB316 CRC64;
     MEGRGFSGLY RNSSEELFLK TVMESPIGMP VPSMEMLGFK NVSQGFRADS EELFKRWLTN
     GEGYNSSSIG FSSRLSKRIS TELVNGSNQL QVGVASDGRN NDKPFIQNNL LANDVSGDFN
     FPIRDPVDRE LQPSNLFLAK AWFLSDQRMT RSRSSELRRR YSEMQNGLAT QGIESICMDP
     QHGAEATKQE VANFNGYNYL SMCELPSQKG SFMSPSNSCS SNFNTPQFGD MDKVSSCVSM
     LKGTLQRRRL SSQLEKEAAE DDLNGIFYPQ EPLFQTGFDQ GQENWSNQTP VNVQVDSIGE
     VKDHGVLQTL EGSTNPVVDG FANQINQIYV GTASGEPSQS ESSNAAPVIS SGLDTCEGPI
     NSNQTLCESS WKQVGVSKSS ENTQNRVKGF REQIMDNLKD DKKRKSLERY GSITSAVSDD
     KGDTTKKRRV ERSRKMAEAK ERNSTPSVPS DMQAVLKRCE NLEKEVRSLK LNLSFMNRKD
     SEQTKQIEDL QKQNEELADE KERLLEEIER ILSETEKM