CCM1_YEAST
ID CCM1_YEAST Reviewed; 864 AA.
AC P48237; D6VUT1;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Mitochondrial group I intron splicing factor CCM1;
DE AltName: Full=COB and COX1 mRNA maturation protein 1;
DE AltName: Full=Required for respiratory growth protein 2;
DE Flags: Precursor;
GN Name=CCM1; Synonyms=DMR1, RRG2; OrderedLocusNames=YGR150C; ORFNames=G6642;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8585325; DOI=10.1002/yea.320111410;
RA Skala J., Nawrocki A., Goffeau A.;
RT "The sequence of a 27 kb segment on the right arm of chromosome VII from
RT Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2,
RT NSR1 genes and ten new open reading frames.";
RL Yeast 11:1421-1427(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16823961; DOI=10.1021/pr050477f;
RA Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT "Toward the complete yeast mitochondrial proteome: multidimensional
RT separation techniques for mitochondrial proteomics.";
RL J. Proteome Res. 5:1543-1554(2006).
RN [7]
RP FUNCTION.
RX PubMed=19562342; DOI=10.1007/s00294-009-0260-z;
RA Moreno J.I., Buie K.S., Price R.E., Piva M.A.;
RT "Ccm1p/Ygr150cp, a pentatricopeptide repeat protein, is essential to remove
RT the fourth intron of both COB and COX1 pre-mRNAs in Saccharomyces
RT cerevisiae.";
RL Curr. Genet. 55:475-484(2009).
RN [8]
RP FUNCTION.
RX PubMed=19751518; DOI=10.1186/gb-2009-10-9-r95;
RA Merz S., Westermann B.;
RT "Genome-wide deletion mutant analysis reveals genes required for
RT respiratory growth, mitochondrial genome maintenance and mitochondrial
RT protein synthesis in Saccharomyces cerevisiae.";
RL Genome Biol. 10:R95.1-R95.18(2009).
RN [9]
RP SUBCELLULAR LOCATION, RNA-BINDING, AND FUNCTION.
RX PubMed=20124025; DOI=10.1534/genetics.110.113969;
RA Puchta O., Lubas M., Lipinski K.A., Piatkowski J., Malecki M., Golik P.;
RT "DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae encodes an RNA-binding
RT protein from the pentatricopeptide repeat family required for the
RT maintenance of the mitochondrial 15S ribosomal RNA.";
RL Genetics 184:959-973(2010).
CC -!- FUNCTION: RNA-binding protein involved in the specific removal of group
CC I introns in mitochondrial encoded transcripts. Maintains the stability
CC of the small subunit mitochondrial 15S rRNA and thus the expression of
CC the mitochondrial genome. {ECO:0000269|PubMed:19562342,
CC ECO:0000269|PubMed:19751518, ECO:0000269|PubMed:20124025}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:16823961, ECO:0000269|PubMed:20124025}.
CC -!- MISCELLANEOUS: Present with 876 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the CCM1 family. {ECO:0000305}.
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DR EMBL; X85807; CAA59808.1; -; Genomic_DNA.
DR EMBL; Z72935; CAA97164.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08242.1; -; Genomic_DNA.
DR PIR; S60441; S60441.
DR RefSeq; NP_011666.1; NM_001181279.1.
DR AlphaFoldDB; P48237; -.
DR BioGRID; 33398; 47.
DR DIP; DIP-6575N; -.
DR IntAct; P48237; 2.
DR STRING; 4932.YGR150C; -.
DR MaxQB; P48237; -.
DR PaxDb; P48237; -.
DR PRIDE; P48237; -.
DR EnsemblFungi; YGR150C_mRNA; YGR150C; YGR150C.
DR GeneID; 853053; -.
DR KEGG; sce:YGR150C; -.
DR SGD; S000003382; CCM1.
DR VEuPathDB; FungiDB:YGR150C; -.
DR eggNOG; ENOG502QUX2; Eukaryota.
DR HOGENOM; CLU_334653_0_0_1; -.
DR InParanoid; P48237; -.
DR OMA; RALYYKY; -.
DR BioCyc; YEAST:G3O-30853-MON; -.
DR PRO; PR:P48237; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P48237; protein.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0019843; F:rRNA binding; IDA:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0000963; P:mitochondrial RNA processing; IMP:SGD.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:2000234; P:positive regulation of rRNA processing; IMP:SGD.
DR GO; GO:0008380; P:RNA splicing; IMP:SGD.
DR GO; GO:0016072; P:rRNA metabolic process; IMP:SGD.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF13041; PPR_2; 1.
DR TIGRFAMs; TIGR00756; PPR; 1.
DR PROSITE; PS51375; PPR; 2.
PE 1: Evidence at protein level;
KW Mitochondrion; mRNA processing; mRNA splicing; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..864
FT /note="Mitochondrial group I intron splicing factor CCM1"
FT /id="PRO_0000202830"
FT REPEAT 319..355
FT /note="PPR 1"
FT REPEAT 356..390
FT /note="PPR 2"
FT REGION 80..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 864 AA; 101423 MW; 5A773DEAA33D84FE CRC64;
MYMARCGPKN NVLCFPFQLS FLFSKRLINK RFKYTLQTED EKNMMGSLSK NKIITPEDVE
FKLAQLREFS NTLKERIHNT KSVNSDGHQS NSIAPISEDS RNVNVTKTSS VPNEEKSKNL
SDLIHSSFLE KMDHLVPKVI RERVADDDIL AKNLFDRSHS NWAPVIDRLY VSEKRFMDID
SREFSVWLNG TVKYLPFHSI LHLDEMLLEQ INGDVVKFNT HMYECIFNNL GNLKPTNFNQ
DGTNDKVILK MKELLERYDK ALKITEERIN KKEGFPSKVP KMTQAILNNC LKYSTKCSSF
HDMDYFITKF RDDYGITPNK QNLTTVIQFY SRKEMTKQAW NTFDTMKFLS TKHFPDICTY
NTMLRICEKE RNFPKALDLF QEIQDHNIKP TTNTYIMMAR VLASSSSNAV VSEGKSDSLR
LLGWKYLHEL EDKNLYRHKK DDLNLFLAMM ALAAFDGDIE LSRALYYLFI AKKYKTLCAN
WKGNILVDQD TIWKSTLMPE MLNYLMLAYA RFDPRNLPVL SGYEKGIELR RKFLREFDSS
MRLDDTDKLV KFKLPFLPIS DLNSEAQVLA ESNAIWSFNM ENGGTRNTLT SSNEAALEDI
KKYRQLLDSF AQEAEDFNEF KFKVMYEVTK MQRESINVNV FNKISLHTYL SIPINLKQQK
EFLRRLTFFT FQQHEFEAVI KRLYEGYRNI PSSHTRDQNS ISTEAISVSK PETTEDLNLI
MHDIWYITCL RHKIMMDTTL YELVMKAAIE FQNEDLAKKV WNDRGKFRTT VPFLKMDQRI
RIAKDQKFAH LMVEFFTKQG KYSDAIAIIL SSKNRFNWTY SMVRNLHKAL EEIEDRNSVE
ILLDVVNKKS HAKALKWEEQ ELNM
