CCMA_ECOLI
ID CCMA_ECOLI Reviewed; 207 AA.
AC P33931; Q2MAP2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Cytochrome c biogenesis ATP-binding export protein CcmA {ECO:0000255|HAMAP-Rule:MF_01707};
DE EC=7.6.2.5 {ECO:0000255|HAMAP-Rule:MF_01707};
DE AltName: Full=Heme exporter protein A {ECO:0000255|HAMAP-Rule:MF_01707};
GN Name=ccmA {ECO:0000255|HAMAP-Rule:MF_01707}; Synonyms=yejW;
GN OrderedLocusNames=b2201, JW5366;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP CHARACTERIZATION, AND GENE NAME.
RX PubMed=7635817; DOI=10.1128/jb.177.15.4321-4326.1995;
RA Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H.;
RT "Escherichia coli genes required for cytochrome c maturation.";
RL J. Bacteriol. 177:4321-4326(1995).
RN [5]
RP CHARACTERIZATION.
RC STRAIN=K12;
RX PubMed=10339610; DOI=10.1073/pnas.96.11.6462;
RA Schulz H., Fabianek R.A., Pellicioli E.C., Hennecke H., Thony-Meyer L.;
RT "Heme transfer to the heme chaperone CcmE during cytochrome c maturation
RT requires the CcmC protein, which may function independently of the ABC-
RT transporter CcmAB.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:6462-6467(1999).
RN [6]
RP CHARACTERIZATION.
RC STRAIN=K12;
RX PubMed=10708391; DOI=10.1099/00221287-146-2-527;
RA Cook G.M., Poole R.K.;
RT "Oxidase and periplasmic cytochrome assembly in Escherichia coli K-12:
RT CydDC and CcmAB are not required for haem-membrane association.";
RL Microbiology 146:527-536(2000).
CC -!- FUNCTION: Part of the ABC transporter complex CcmAB involved in the
CC biogenesis of c-type cytochromes; once thought to export heme, this
CC seems not to be the case, but its exact role is uncertain. Responsible
CC for energy coupling to the transport system.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + heme b(in) = ADP + H(+) + heme b(out) + phosphate;
CC Xref=Rhea:RHEA:19261, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:60344,
CC ChEBI:CHEBI:456216; EC=7.6.2.5; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01707};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (CcmA) and
CC two transmembrane proteins (CcmB). {ECO:0000255|HAMAP-Rule:MF_01707}.
CC -!- INTERACTION:
CC P33931; P0ABL8: ccmB; NbExp=2; IntAct=EBI-6402796, EBI-546936;
CC P33931; P0ABM1: ccmC; NbExp=4; IntAct=EBI-6402796, EBI-2123469;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01707}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01707}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. CcmA exporter
CC (TC 3.A.1.107) family. {ECO:0000255|HAMAP-Rule:MF_01707}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA16393.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00008; AAA16393.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC75261.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE76664.1; -; Genomic_DNA.
DR PIR; G64989; G64989.
DR RefSeq; NP_416705.2; NC_000913.3.
DR RefSeq; WP_000525587.1; NZ_STEB01000002.1.
DR AlphaFoldDB; P33931; -.
DR SMR; P33931; -.
DR BioGRID; 4261550; 10.
DR BioGRID; 851055; 2.
DR ComplexPortal; CPX-3568; CcmABCDE system I cytochrome c biogenesis complex.
DR IntAct; P33931; 5.
DR STRING; 511145.b2201; -.
DR jPOST; P33931; -.
DR PaxDb; P33931; -.
DR PRIDE; P33931; -.
DR EnsemblBacteria; AAC75261; AAC75261; b2201.
DR EnsemblBacteria; BAE76664; BAE76664; BAE76664.
DR GeneID; 66673904; -.
DR GeneID; 946714; -.
DR KEGG; ecj:JW5366; -.
DR KEGG; eco:b2201; -.
DR PATRIC; fig|511145.12.peg.2290; -.
DR EchoBASE; EB1989; -.
DR eggNOG; COG4133; Bacteria.
DR HOGENOM; CLU_000604_1_2_6; -.
DR InParanoid; P33931; -.
DR OMA; NLAWLCA; -.
DR PhylomeDB; P33931; -.
DR BioCyc; EcoCyc:CCMA-MON; -.
DR BioCyc; MetaCyc:CCMA-MON; -.
DR PRO; PR:P33931; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IC:ComplexPortal.
DR GO; GO:0005829; C:cytosol; ISM:EcoCyc.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IDA:EcoCyc.
DR GO; GO:0015439; F:ABC-type heme transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IDA:EcoCyc.
DR GO; GO:1903607; P:cytochrome c biosynthetic process; IDA:EcoCyc.
DR GO; GO:0017004; P:cytochrome complex assembly; IC:ComplexPortal.
DR GO; GO:1904334; P:heme import across plasma membrane; IC:ComplexPortal.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR005895; ABC_transptr_haem_export_CcmA.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43499; PTHR43499; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01189; ccmA; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51243; CCMA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell inner membrane; Cell membrane;
KW Cytochrome c-type biogenesis; Membrane; Nucleotide-binding;
KW Reference proteome; Translocase; Transport.
FT CHAIN 1..207
FT /note="Cytochrome c biogenesis ATP-binding export protein
FT CcmA"
FT /id="PRO_0000092177"
FT DOMAIN 4..207
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01707"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01707"
SQ SEQUENCE 207 AA; 23053 MW; 73224210C6FAEC4A CRC64;
MGMLEARELL CERDERTLFS GLSFTLNAGE WVQITGSNGA GKTTLLRLLT GLSRPDAGEV
LWQGQPLHQV RDSYHQNLLW IGHQPGIKTR LTALENLHFY HRDGDTAQCL EALAQAGLAG
FEDIPVNQLS AGQQRRVALA RLWLTRATLW ILDEPFTAID VNGVDRLTQR MAQHTEQGGI
VILTTHQPLN VAESKIRRIS LTQTRAA
