CCME_ARATH
ID CCME_ARATH Reviewed; 256 AA.
AC Q96326;
DT 31-JAN-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Cytochrome c-type biogenesis protein CcmE homolog, mitochondrial {ECO:0000305};
DE AltName: Full=Cytochrome c maturation protein E homolog {ECO:0000305};
DE Short=AtCCME {ECO:0000303|PubMed:11069919};
DE AltName: Full=Heme chaperone CcmE homolog {ECO:0000305};
DE Flags: Precursor;
GN Name=CCME {ECO:0000303|PubMed:11069919};
GN Synonyms=ATG1 {ECO:0000312|EMBL:AAB18126.1};
GN OrderedLocusNames=At3g51790 {ECO:0000312|Araport:AT3G51790};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Grellet F., Gaubier P., Wu H.-J., Laudie M., Berger C., Delseny M.;
RT "Structure of the Arabidopsis thaliana Em1 locus.";
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10645728; DOI=10.1023/a:1006395324818;
RA Comella P., Wu H.-J., Laudie M., Berger C., Cooke R., Delseny M.,
RA Grellet F.;
RT "Fine sequence analysis of 60 kb around the Arabidopsis thaliana AtEm1
RT locus on chromosome III.";
RL Plant Mol. Biol. 41:687-700(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, HEME BINDING, AND MUTAGENESIS OF HIS-222.
RX PubMed=11069919; DOI=10.1074/jbc.m008853200;
RA Spielewoy N., Schulz H., Grienenberger J.M., Thony-Meyer L., Bonnard G.;
RT "CCME, a nuclear-encoded heme-binding protein involved in cytochrome c
RT maturation in plant mitochondria.";
RL J. Biol. Chem. 276:5491-5497(2001).
CC -!- FUNCTION: Heme-binding chaperone that may be involved in cytochrome c
CC maturation in mitochondria. {ECO:0000269|PubMed:11069919}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:11069919}; Single-pass membrane protein
CC {ECO:0000255}. Mitochondrion intermembrane space
CC {ECO:0000269|PubMed:11069919}. Note=According to some authors, CCME is
CC a peripheral mitochondrion inner membrane protein with the hydrophilic
CC domain oriented toward the mitochondrion intermembrane space
CC (Pubmed:11069919). {ECO:0000269|PubMed:11069919}.
CC -!- SIMILARITY: Belongs to the CcmE/CycJ family. {ECO:0000255|HAMAP-
CC Rule:MF_01959}.
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DR EMBL; U72502; AAB18126.1; -; mRNA.
DR EMBL; AF049236; AAC14406.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78843.1; -; Genomic_DNA.
DR EMBL; BT004607; AAO42853.1; -; mRNA.
DR EMBL; AK227474; BAE99476.1; -; mRNA.
DR PIR; T51150; T51150.
DR RefSeq; NP_190747.1; NM_115038.3.
DR AlphaFoldDB; Q96326; -.
DR SMR; Q96326; -.
DR IntAct; Q96326; 27.
DR STRING; 3702.AT3G51790.1; -.
DR iPTMnet; Q96326; -.
DR PaxDb; Q96326; -.
DR PRIDE; Q96326; -.
DR ProteomicsDB; 224444; -.
DR EnsemblPlants; AT3G51790.1; AT3G51790.1; AT3G51790.
DR GeneID; 824342; -.
DR Gramene; AT3G51790.1; AT3G51790.1; AT3G51790.
DR KEGG; ath:AT3G51790; -.
DR Araport; AT3G51790; -.
DR TAIR; locus:2074363; AT3G51790.
DR eggNOG; ENOG502QTHD; Eukaryota.
DR HOGENOM; CLU_079503_0_0_1; -.
DR InParanoid; Q96326; -.
DR OMA; ARRLECY; -.
DR OrthoDB; 1332874at2759; -.
DR PhylomeDB; Q96326; -.
DR PRO; PR:Q96326; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q96326; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:TAIR.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-KW.
DR GO; GO:0017003; P:protein-heme linkage; IEA:InterPro.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_01959; CcmE; 1.
DR InterPro; IPR004329; CcmE.
DR InterPro; IPR036127; CcmE-like_sf.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR PANTHER; PTHR34128; PTHR34128; 1.
DR Pfam; PF03100; CcmE; 1.
DR SUPFAM; SSF82093; SSF82093; 1.
PE 1: Evidence at protein level;
KW Cytochrome c-type biogenesis; Heme; Iron; Membrane; Metal-binding;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..57
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 58..256
FT /note="Cytochrome c-type biogenesis protein CcmE homolog,
FT mitochondrial"
FT /evidence="ECO:0000255"
FT /id="PRO_0000443062"
FT TRANSMEM 84..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 222
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000269|PubMed:11069919"
FT BINDING 226
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P69490"
FT MUTAGEN 222
FT /note="H->A: Abolishes heme binding."
FT /evidence="ECO:0000269|PubMed:11069919"
SQ SEQUENCE 256 AA; 28600 MW; 09B8249151F734BC CRC64;
MAARLLFRRS SQILRSIQRN PQISSSFESP PCPIFHSLTT ASPDPSRLSS LTFLRSLSIA
RRGPTRPKKI DIGAKARQMQ NRRLWTYALT FSCIAGFVVI VLNQFQDQLV FYLTPSDAME
KFAENPTKNK FRLGGLVLEG SVAQPAASQE MEFVITDLIT DILVRYKGSL PDLFREGHSV
VVEGFIKPYT DEVRKEVSTK PISKKARNLD CFFSATEVLA KHDEKYMPQE VAAAIEKNKK
IIEAAATEQA AEVAAS
