CCME_GLUDA
ID CCME_GLUDA Reviewed; 173 AA.
AC Q69AY8; A9H406; B5ZID1;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Cytochrome c-type biogenesis protein CcmE;
DE AltName: Full=Cytochrome c maturation protein E;
DE AltName: Full=Heme chaperone CcmE;
GN Name=ccmE; Synonyms=cycJ; OrderedLocusNames=GDI3440, Gdia_2933;
OS Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298
OS / CIP 103539 / LMG 7603 / PAl5).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC Acetobacteraceae; Gluconacetobacter.
OX NCBI_TaxID=272568;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=15292139; DOI=10.1128/jb.186.16.5384-5391.2004;
RA Lee S., Flores-Encarnacion M., Contreras-Zentella M., Garcia-Flores L.,
RA Escamilla J.E., Kennedy C.;
RT "Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter
RT diazotrophicus strains with mutations in cytochrome c biogenesis genes.";
RL J. Bacteriol. 186:5384-5391(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5;
RX PubMed=19775431; DOI=10.1186/1471-2164-10-450;
RA Bertalan M., Albano R., de Padua V., Rouws L., Rojas C., Hemerly A.,
RA Teixeira K., Schwab S., Araujo J., Oliveira A., Franca L., Magalhaes V.,
RA Alqueres S., Cardoso A., Almeida W., Loureiro M.M., Nogueira E., Cidade D.,
RA Oliveira D., Simao T., Macedo J., Valadao A., Dreschsel M., Freitas F.,
RA Vidal M., Guedes H., Rodrigues E., Meneses C., Brioso P., Pozzer L.,
RA Figueiredo D., Montano H., Junior J., de Souza Filho G.,
RA Martin Quintana Flores V., Ferreira B., Branco A., Gonzalez P.,
RA Guillobel H., Lemos M., Seibel L., Macedo J., Alves-Ferreira M.,
RA Sachetto-Martins G., Coelho A., Santos E., Amaral G., Neves A.,
RA Pacheco A.B., Carvalho D., Lery L., Bisch P., Rossle S.C., Urmenyi T.,
RA Rael Pereira A., Silva R., Rondinelli E., von Kruger W., Martins O.,
RA Baldani J.I., Ferreira P.C.;
RT "Complete genome sequence of the sugarcane nitrogen-fixing endophyte
RT Gluconacetobacter diazotrophicus Pal5.";
RL BMC Genomics 10:450-450(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5;
RX PubMed=21304715; DOI=10.4056/sigs.972221;
RA Giongo A., Tyler H.L., Zipperer U.N., Triplett E.W.;
RT "Two genome sequences of the same bacterial strain, Gluconacetobacter
RT diazotrophicus PAl 5, suggest a new standard in genome sequence
RT submission.";
RL Stand. Genomic Sci. 2:309-317(2010).
CC -!- FUNCTION: Heme chaperone required for the biogenesis of c-type
CC cytochromes. Transiently binds heme delivered by CcmC and transfers the
CC heme to apo-cytochromes in a process facilitated by CcmF and CcmH (By
CC similarity). Also required for indole-3-acetic acid (IAA) biosynthesis.
CC {ECO:0000250, ECO:0000269|PubMed:15292139}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC type II membrane protein {ECO:0000305}; Periplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CcmE/CycJ family. {ECO:0000305}.
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DR EMBL; AY456185; AAS13482.1; -; Genomic_DNA.
DR EMBL; AM889285; CAP57383.1; -; Genomic_DNA.
DR EMBL; CP001189; ACI52664.1; -; Genomic_DNA.
DR RefSeq; WP_012228035.1; NC_011365.1.
DR AlphaFoldDB; Q69AY8; -.
DR SMR; Q69AY8; -.
DR STRING; 272568.GDI3440; -.
DR PRIDE; Q69AY8; -.
DR EnsemblBacteria; ACI52664; ACI52664; Gdia_2933.
DR EnsemblBacteria; CAP57383; CAP57383; GDI3440.
DR KEGG; gdi:GDI3440; -.
DR KEGG; gdj:Gdia_2933; -.
DR eggNOG; COG2332; Bacteria.
DR HOGENOM; CLU_079503_1_1_5; -.
DR OMA; HVEFAVH; -.
DR OrthoDB; 1724595at2; -.
DR Proteomes; UP000000736; Chromosome.
DR Proteomes; UP000001176; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-KW.
DR GO; GO:0017003; P:protein-heme linkage; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_01959; CcmE; 1.
DR InterPro; IPR004329; CcmE.
DR InterPro; IPR036127; CcmE-like_sf.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR PANTHER; PTHR34128; PTHR34128; 1.
DR Pfam; PF03100; CcmE; 1.
DR SUPFAM; SSF82093; SSF82093; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cytochrome c-type biogenesis; Heme;
KW Iron; Membrane; Metal-binding; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..173
FT /note="Cytochrome c-type biogenesis protein CcmE"
FT /id="PRO_0000238789"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..28
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..173
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT REGION 148..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 125
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000250"
FT BINDING 129
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 173 AA; 18539 MW; DD4240F824E3B727 CRC64;
MTRKSRRLWL VGACLLGLGT ATALVLNAFS SNIVFFMAPS QVRAHPPAAD RTIRLGGMVV
AGSVRRQTQG DTPIALFDVT DGQAAVTVRY AGILPDLFRE GQSVVAVGTV APDGAFRASE
VLAKHDETYM PKEVAEALRR SGKWDPRYGK APDAASWNTM TVGDARHGEA NRS
