ID   1AP1_BRAOT              Reviewed;         256 AA.
AC   Q96355;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Floral homeotic protein APETALA 1-1;
DE            Short=Boi1AP1;
DE   AltName: Full=Agamous-like MADS-box protein 1AP1;
GN   Name=1AP1;
OS   Brassica oleracea var. italica (Broccoli).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=36774;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Flower;
RX   PubMed=9084216; DOI=10.1007/bf01007702;
RA   Carr S.M., Irish V.F.;
RT   "Floral homeotic gene expression defines developmental arrest stages in
RT   Brassica oleracea L. vars. botrytis and italica.";
RL   Planta 201:179-188(1997).
CC   -!- FUNCTION: Transcription factor that promotes early floral meristem
CC       identity in synergy with LEAFY. Displays a redundant function with
CC       CAULIFLOWER in the up-regulation of LEAFY. Required subsequently for
CC       the transition of an inflorescence meristem into a floral meristem, and
CC       for the normal development of sepals and petals in flowers. Regulates
CC       positively B class homeotic proteins (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC   -!- TISSUE SPECIFICITY: Expressed in some of the meristems of arrest-stage
CC       broccoli heads. {ECO:0000269|PubMed:9084216}.
CC   -!- DEVELOPMENTAL STAGE: First observed in young floral meristem before
CC       organs initiation. Accumulates strongly in the sepals of the early
CC       stage bud. In more mature buds, expressed on the adaxial side of the
CC       sepals, in the petal primordia, and transiently in young stamen
CC       primordia. {ECO:0000269|PubMed:9084216}.
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DR   EMBL; U67451; AAB08875.1; -; mRNA.
DR   AlphaFoldDB; Q96355; -.
DR   SMR; Q96355; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002487; TF_Kbox.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF01486; K-box; 1.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS51297; K_BOX; 1.
DR   PROSITE; PS00350; MADS_BOX_1; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Developmental protein; Differentiation;
KW   DNA-binding; Flowering; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..256
FT                   /note="Floral homeotic protein APETALA 1-1"
FT                   /id="PRO_0000417135"
FT   DOMAIN          1..61
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT   DOMAIN          88..178
FT                   /note="K-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
SQ   SEQUENCE   256 AA;  30123 MW;  0ECE9490DD089854 CRC64;
     MGRGRVQLKR IENKINRQVT FSKRRAGLFK KAHEISVLCD AEVALVVFSH KGKLFEYSTD
     SCMEKILERY ERYSYAERQL IAPESDVNTN WSMEYNRLKA KIELLERNQR HYLGEDLQAM
     SPKELQNLEQ QLDTALKHIR SRKNQLMYDS VNELQRKEKA IQEQNSMLSK QIKEREKVLM
     AQQEQWDQQN HGQNMPSPPP PQQHQIQHPY MLSHQPSPFL NMGGLYQEED PMAMRRNDLD
     LSLEPVYNCN LGCFAA