CCME_RHIEC
ID CCME_RHIEC Reviewed; 155 AA.
AC Q2JYF0; Q52731; Q9RNX4;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Cytochrome c-type biogenesis protein CcmE {ECO:0000255|HAMAP-Rule:MF_01959};
DE AltName: Full=Cytochrome c maturation protein E {ECO:0000255|HAMAP-Rule:MF_01959};
DE AltName: Full=Heme chaperone CcmE {ECO:0000255|HAMAP-Rule:MF_01959};
GN Name=ccmE1 {ECO:0000255|HAMAP-Rule:MF_01959};
GN Synonyms=cycJ1 {ECO:0000255|HAMAP-Rule:MF_01959};
GN OrderedLocusNames=RHE_CH01290;
GN and
GN Name=ccmE2 {ECO:0000255|HAMAP-Rule:MF_01959};
GN Synonyms=cycJ2 {ECO:0000255|HAMAP-Rule:MF_01959};
GN OrderedLocusNames=RHE_PF00497;
OS Rhizobium etli (strain CFN 42 / ATCC 51251).
OG Plasmid p42f.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX NCBI_TaxID=347834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CFN 42 / ATCC 51251;
RX PubMed=9524269; DOI=10.1016/s0378-1119(97)00655-0;
RA Tabche M.-L., Garcia E.G., Miranda J., Escamilla J.E., Soberon M.;
RT "Rhizobium etli cycHJKL gene locus involved in c-type cytochrome
RT biogenesis: sequence analysis and characterization of two cycH mutants.";
RL Gene 208:215-219(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CE3;
RX PubMed=10854788; DOI=10.1016/s0378-1119(00)00176-1;
RA Reyes J.D., Tabche M.-L., Morera C., de Lourdes Girard M., Romero D.,
RA Krol E., Miranda J., Soberon M.;
RT "Expression pattern of Rhizobium etli ccmIEFH genes involved in c-type
RT cytochrome maturation.";
RL Gene 250:149-157(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CFN 42 / ATCC 51251;
RX PubMed=16505379; DOI=10.1073/pnas.0508502103;
RA Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.,
RA Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A.,
RA Jimenez-Jacinto V., Collado-Vides J., Davila G.;
RT "The partitioned Rhizobium etli genome: genetic and metabolic redundancy in
RT seven interacting replicons.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006).
CC -!- FUNCTION: Heme chaperone required for the biogenesis of c-type
CC cytochromes. Transiently binds heme delivered by CcmC and transfers the
CC heme to apo-cytochromes in a process facilitated by CcmF and CcmH.
CC {ECO:0000255|HAMAP-Rule:MF_01959}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01959}; Single-pass type II membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01959}; Periplasmic side {ECO:0000255|HAMAP-
CC Rule:MF_01959}.
CC -!- SIMILARITY: Belongs to the CcmE/CycJ family. {ECO:0000255|HAMAP-
CC Rule:MF_01959}.
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DR EMBL; U45318; AAC46237.1; -; Genomic_DNA.
DR EMBL; AF176798; AAF00988.1; -; Genomic_DNA.
DR EMBL; CP000138; ABC94386.1; -; Genomic_DNA.
DR EMBL; CP000133; ABC90095.1; -; Genomic_DNA.
DR RefSeq; WP_011424629.1; NC_007766.1.
DR AlphaFoldDB; Q2JYF0; -.
DR SMR; Q2JYF0; -.
DR STRING; 347834.RHE_CH01290; -.
DR EnsemblBacteria; ABC90095; ABC90095; RHE_CH01290.
DR EnsemblBacteria; ABC94386; ABC94386; RHE_PF00497.
DR KEGG; ret:RHE_CH01290; -.
DR KEGG; ret:RHE_PF00497; -.
DR eggNOG; COG2332; Bacteria.
DR HOGENOM; CLU_079503_1_1_5; -.
DR OMA; HVEFAVH; -.
DR Proteomes; UP000001936; Chromosome.
DR Proteomes; UP000001936; Plasmid p42f.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-KW.
DR GO; GO:0017003; P:protein-heme linkage; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_01959; CcmE; 1.
DR InterPro; IPR004329; CcmE.
DR InterPro; IPR036127; CcmE-like_sf.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR PANTHER; PTHR34128; PTHR34128; 1.
DR Pfam; PF03100; CcmE; 1.
DR SUPFAM; SSF82093; SSF82093; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cytochrome c-type biogenesis; Heme;
KW Iron; Membrane; Metal-binding; Plasmid; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..155
FT /note="Cytochrome c-type biogenesis protein CcmE"
FT /id="PRO_0000238848"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01959"
FT TRANSMEM 8..28
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01959"
FT TOPO_DOM 29..155
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01959"
FT BINDING 124
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01959"
FT BINDING 128
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01959"
FT CONFLICT 20..22
FT /note="AAV -> VAL (in Ref. 2; AAF00988)"
FT /evidence="ECO:0000305"
FT CONFLICT 28
FT /note="A -> P (in Ref. 2; AAF00988)"
FT /evidence="ECO:0000305"
FT CONFLICT 34
FT /note="A -> P (in Ref. 2; AAF00988)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="V -> L (in Ref. 2; AAF00988)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="Missing (in Ref. 2; AAC46237)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="Missing (in Ref. 2; AAC46237)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 155 AA; 16488 MW; A8AFAAC491D20AAA CRC64;
MTRKQKRLVV IAGGMSFILA AVLLVMFAFS QSVAYFYMPA DLARTPVAPE TRIRLGGLVG
AGSVVRGAGS TVEFSVTDGS ANAVKVKYTG ILPDLFREGQ GVVTEGMFAA GSNVFIADTV
LAKHDETYMP KEVADRLKSQ GLWQEGKGQE AKATP
