CCMF2_ARATH
ID CCMF2_ARATH Reviewed; 203 AA.
AC Q33884; A7KNF1; O03602; Q9T6H7;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Cytochrome c biogenesis CcmF N-terminal-like mitochondrial protein 2 {ECO:0000305};
DE AltName: Full=Cytochrome c biogenesis orf203 {ECO:0000312|EMBL:CAA69836.3};
DE Flags: Fragment;
GN Name=CCMFN2 {ECO:0000303|PubMed:18644794};
GN Synonyms=CC6BN2, CCB203 {ECO:0000312|EMBL:CAA69836.3};
GN OrderedLocusNames=AtMg00960;
OS Arabidopsis thaliana (Mouse-ear cress).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. C24;
RA Schuster W.;
RL Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=8988169; DOI=10.1038/ng0197-57;
RA Unseld M., Marienfeld J.R., Brandt P., Brennicke A.;
RT "The mitochondrial genome of Arabidopsis thaliana contains 57 genes in
RT 366,924 nucleotides.";
RL Nat. Genet. 15:57-61(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07768).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 8-193, AND RNA EDITING.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta; TISSUE=Rosette leaf;
RX PubMed=17565941; DOI=10.1534/genetics.107.073585;
RA Bentolila S., Elliott L.E., Hanson M.R.;
RT "Genetic architecture of mitochondrial editing in Arabidopsis thaliana.";
RL Genetics 178:1693-1708(2008).
RN [5]
RP RNA EDITING.
RX PubMed=10611383; DOI=10.1073/pnas.96.26.15324;
RA Giege P., Brennicke A.;
RT "RNA editing in Arabidopsis mitochondria effects 441 C to U changes in
RT ORFs.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999).
RN [6]
RP FUNCTION, INTERACTION WITH CCMFC; CCMFN1; CCMH AND CYTC-1, AND SUBCELLULAR
RP LOCATION.
RX PubMed=18644794; DOI=10.1074/jbc.m802621200;
RA Rayapuram N., Hagenmuller J., Grienenberger J.M., Bonnard G., Giege P.;
RT "The three mitochondrial encoded CcmF proteins form a complex that
RT interacts with CCMH and c-type apocytochromes in Arabidopsis.";
RL J. Biol. Chem. 283:25200-25208(2008).
CC -!- FUNCTION: Forms a complex with CCMFC, CCMFN1 and CCMH that performs the
CC assembly of heme with c-type apocytochromes in mitochondria.
CC {ECO:0000269|PubMed:18644794}.
CC -!- SUBUNIT: Interacts with CCMFC, CCMFN1, CCMH and CYTC-1.
CC {ECO:0000269|PubMed:18644794}.
CC -!- INTERACTION:
CC Q33884; P93286: CCMFC; NbExp=4; IntAct=EBI-763400, EBI-1797481;
CC Q33884; Q9T6H8: CCMFN1; NbExp=5; IntAct=EBI-763400, EBI-1797466;
CC Q33884; Q9XI46: CCMH; NbExp=3; IntAct=EBI-763400, EBI-763363;
CC Q33884; Q9FKS5: CYC1-2; NbExp=4; IntAct=EBI-763400, EBI-1777995;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:18644794}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- RNA EDITING: Modified_positions=22 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 59 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 70 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 76 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 87 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 93 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 101 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 107 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 115 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 119 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 131 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941}, 156 {ECO:0000269|PubMed:10611383,
CC ECO:0000269|PubMed:17565941};
CC -!- MISCELLANEOUS: A stretch of 270 kb of the mitochondrial genome is
CC duplicated within the centromere of chromosome 2 resulting in the
CC duplication of the gene. The expression of this duplicated gene
CC (At2g07768) is not demonstrated. It is also probably not RNA edited and
CC therefore differs in all the positions known to be edited.
CC -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC {ECO:0000305}.
CC -!- CAUTION: Ref.1 indicates that this sequence represents the C-terminal
CC part of a protein produced after trans-splicing. No experimental data
CC confirms this assertion. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AC007143; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X98302; CAA66946.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Y08501; CAA69836.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AC007143; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC007730; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; EF488894; ABS50606.1; -; mRNA.
DR EMBL; EF488895; ABS50607.1; -; mRNA.
DR RefSeq; NP_085550.2; NC_001284.2.
DR AlphaFoldDB; Q33884; -.
DR SMR; Q33884; -.
DR IntAct; Q33884; 5.
DR STRING; 3702.ATMG00960.1; -.
DR SwissPalm; Q33884; -.
DR PeptideAtlas; Q33884; -.
DR PRIDE; Q33884; -.
DR Araport; ATMG00960; -.
DR eggNOG; ENOG502QQ5D; Eukaryota.
DR HOGENOM; CLU_024111_0_0_1; -.
DR InParanoid; Q33884; -.
DR OrthoDB; 1139435at2759; -.
DR PhylomeDB; Q33884; -.
DR Proteomes; UP000006548; Mitochondrion (cv. C24).
DR ExpressionAtlas; Q33884; baseline and differential.
DR Genevisible; Q33884; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0015232; F:heme transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0017004; P:cytochrome complex assembly; TAS:UniProtKB.
DR InterPro; IPR002541; Cyt_c_assembly.
DR InterPro; IPR003567; Cyt_c_biogenesis.
DR InterPro; IPR003569; Cyt_c_biogenesis_plant.
DR PANTHER; PTHR43653; PTHR43653; 1.
DR Pfam; PF01578; Cytochrom_C_asm; 1.
DR PRINTS; PR01410; CCBIOGENESIS.
DR PRINTS; PR01412; CCBSBIOGNSIS.
PE 1: Evidence at protein level;
KW Cytochrome c-type biogenesis; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; RNA editing;
KW Transmembrane; Transmembrane helix.
FT CHAIN <1..203
FT /note="Cytochrome c biogenesis CcmF N-terminal-like
FT mitochondrial protein 2"
FT /id="PRO_0000201595"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 21
FT /note="T -> S (in Ref. 3; AC007730)"
FT /evidence="ECO:0000305"
FT NON_TER 1
SQ SEQUENCE 203 AA; 23629 MW; AF3282E8F7524B81 CRC64;
VDTGREQAKR VVRNGKKETT TLPLCWTAGA NTVVSDQDQE PIRIWILTCW WFLTVGILLG
SWWAYHELGW GGWWFWDPVE NASFMPWVLA TACIHSVILP FLHSWTLFLN IVTFLCCVLG
TFSIRSGLLA SVHSFATDDT RGIFLWWFFL LMTGIFMILF YQMKQQASVR RTYKKEMVVA
RSTLVHLRHS ARAQPRPVML WKN
