CCMFC_ARATH
ID CCMFC_ARATH Reviewed; 442 AA.
AC P93286;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Cytochrome c biogenesis CcmF C-terminal-like mitochondrial protein {ECO:0000305};
DE AltName: Full=Cytochrome c biogenesis orf452 {ECO:0000312|EMBL:CAA69763.3};
GN Name=CCMFC; Synonyms=CCB452 {ECO:0000312|EMBL:CAA69763.3}, CCB6C;
GN OrderedLocusNames=AtMg00180;
OS Arabidopsis thaliana (Mouse-ear cress).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. C24;
RX PubMed=8988169; DOI=10.1038/ng0197-57;
RA Unseld M., Marienfeld J.R., Brandt P., Brennicke A.;
RT "The mitochondrial genome of Arabidopsis thaliana contains 57 genes in
RT 366,924 nucleotides.";
RL Nat. Genet. 15:57-61(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND RNA EDITING.
RX PubMed=10611383; DOI=10.1073/pnas.96.26.15324;
RA Giege P., Brennicke A.;
RT "RNA editing in Arabidopsis mitochondria effects 441 C to U changes in
RT ORFs.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07723).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP FUNCTION, INTERACTION WITH CCMFN2, AND SUBCELLULAR LOCATION.
RX PubMed=18644794; DOI=10.1074/jbc.m802621200;
RA Rayapuram N., Hagenmuller J., Grienenberger J.M., Bonnard G., Giege P.;
RT "The three mitochondrial encoded CcmF proteins form a complex that
RT interacts with CCMH and c-type apocytochromes in Arabidopsis.";
RL J. Biol. Chem. 283:25200-25208(2008).
CC -!- FUNCTION: Forms a complex with CCMFN1, CCMFN2 and CCMH that performs
CC the assembly of heme with c-type apocytochromes in mitochondria.
CC {ECO:0000269|PubMed:18644794}.
CC -!- SUBUNIT: Interacts with CCMFN2. {ECO:0000269|PubMed:18644794}.
CC -!- INTERACTION:
CC P93286; Q33884: CCMFN2; NbExp=4; IntAct=EBI-1797481, EBI-763400;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:18644794}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- RNA EDITING: Modified_positions=17 {ECO:0000269|PubMed:10611383}, 35
CC {ECO:0000269|PubMed:10611383}, 41 {ECO:0000269|PubMed:10611383}, 49
CC {ECO:0000269|PubMed:10611383}, 52 {ECO:0000269|PubMed:10611383}, 54
CC {ECO:0000269|PubMed:10611383}, 112 {ECO:0000269|PubMed:10611383}, 136
CC {ECO:0000269|PubMed:10611383}, 139 {ECO:0000269|PubMed:10611383}, 309
CC {ECO:0000269|PubMed:10611383}, 391 {ECO:0000269|PubMed:10611383}, 416
CC {ECO:0000269|PubMed:10611383}, 427 {ECO:0000269|PubMed:10611383}, 443
CC {ECO:0000269|PubMed:10611383}; Note=The stop codon at position 443 is
CC created by RNA editing.;
CC -!- MISCELLANEOUS: A stretch of 270 kb of the mitochondrial genome is
CC duplicated within the centromere of chromosome 2 resulting in the
CC duplication of the gene. The expression of the duplicated gene
CC (At2g07723) is not demonstrated. It is also probably not RNA edited and
CC therefore differs in all the positions known to be edited.
CC -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC {ECO:0000305}.
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DR EMBL; Y08501; CAA69763.3; ALT_SEQ; Genomic_DNA.
DR EMBL; AC006225; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_085489.1; NC_001284.2.
DR AlphaFoldDB; P93286; -.
DR IntAct; P93286; 2.
DR STRING; 3702.ATMG00180.1; -.
DR TCDB; 9.B.14.1.6; the putative heme handling protein (hhp) family.
DR SwissPalm; P93286; -.
DR PaxDb; P93286; -.
DR PeptideAtlas; P93286; -.
DR PRIDE; P93286; -.
DR Araport; ATMG00180; -.
DR eggNOG; KOG0017; Eukaryota.
DR InParanoid; P93286; -.
DR OrthoDB; 643003at2759; -.
DR PRO; PR:P93286; -.
DR Proteomes; UP000006548; Mitochondrion (cv. C24).
DR ExpressionAtlas; P93286; differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-KW.
DR InterPro; IPR032523; CcmF_C.
DR InterPro; IPR044955; CCMFC.
DR PANTHER; PTHR36010; PTHR36010; 1.
DR Pfam; PF16327; CcmF_C; 2.
PE 1: Evidence at protein level;
KW Cytochrome c-type biogenesis; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; RNA editing;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..442
FT /note="Cytochrome c biogenesis CcmF C-terminal-like
FT mitochondrial protein"
FT /id="PRO_0000201593"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 151..175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..175
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 442 AA; 50548 MW; AFDAA24F80423BD4 CRC64;
MVQLHNFFFF IIFMVVLCGT AAPVLLKWFV SRDVSTGAPF FNGTIIPILI SLFSLLVYLH
SRKIIRSMDG AKSGVLVRAS RPILLPDIIG RSSSETRARK ALFFFVPVLH FCLLESKGDF
SYLESFCGVL CLLFFCTFFF LARDRSAKRE RARRRKGQTL RPNGNEQRRN DKMRCSGHPH
LDLERRVEGF GPLAFPVPPE LGGACVGGVP PEIGLEALAL PRSRQLMAMA VGHDYYQKVP
MKMNISHGGV CICMLGVLLS NTKKIQFTQR LPLGYELHMG KERCCLRGLD HLHGPTFHSI
CGNLMIYKSS LTNDRLMFEH DESLHADLLL INFPASYKNG KLEHFLHWWM KNRKHNNFWL
TMFPEKRYFR ERTSTAEVAI HTNLFTDLYA LIGTGSSRTG GWYTTIMKLP FIFFIWIGFM
LASLGGLPSL LRQLQKDKLR WN
