CCMF_ECOLI
ID CCMF_ECOLI Reviewed; 647 AA.
AC P33927; Q2MAP7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Cytochrome c-type biogenesis protein CcmF;
GN Name=ccmF; Synonyms=yejR; OrderedLocusNames=b2196, JW2184;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP CHARACTERIZATION, AND GENE NAME.
RX PubMed=7635817; DOI=10.1128/jb.177.15.4321-4326.1995;
RA Thoeny-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H.;
RT "Escherichia coli genes required for cytochrome c maturation.";
RL J. Bacteriol. 177:4321-4326(1995).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Required for the biogenesis of c-type cytochromes. Possible
CC subunit of a heme lyase.
CC -!- INTERACTION:
CC P33927; P0ABM9: ccmH; NbExp=3; IntAct=EBI-763370, EBI-560309;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC {ECO:0000305}.
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DR EMBL; U00008; AAA16388.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75256.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76659.1; -; Genomic_DNA.
DR PIR; B64989; B64989.
DR RefSeq; NP_416700.1; NC_000913.3.
DR RefSeq; WP_000982447.1; NZ_SSZK01000027.1.
DR AlphaFoldDB; P33927; -.
DR SMR; P33927; -.
DR BioGRID; 4260482; 42.
DR ComplexPortal; CPX-3569; CcmFH system I cytochrome c biogenesis complex.
DR DIP; DIP-9256N; -.
DR IntAct; P33927; 3.
DR MINT; P33927; -.
DR STRING; 511145.b2196; -.
DR TCDB; 9.B.14.1.3; the putative heme handling protein (hhp) family.
DR PaxDb; P33927; -.
DR PRIDE; P33927; -.
DR EnsemblBacteria; AAC75256; AAC75256; b2196.
DR EnsemblBacteria; BAE76659; BAE76659; BAE76659.
DR GeneID; 948783; -.
DR KEGG; ecj:JW2184; -.
DR KEGG; eco:b2196; -.
DR PATRIC; fig|1411691.4.peg.40; -.
DR EchoBASE; EB1985; -.
DR eggNOG; COG1138; Bacteria.
DR HOGENOM; CLU_015041_3_0_6; -.
DR InParanoid; P33927; -.
DR OMA; SYWGMQL; -.
DR PhylomeDB; P33927; -.
DR BioCyc; EcoCyc:EG12054-MON; -.
DR BioCyc; MetaCyc:EG12054-MON; -.
DR PRO; PR:P33927; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0020037; F:heme binding; IMP:EcoCyc.
DR GO; GO:0015232; F:heme transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0018063; P:cytochrome c-heme linkage; IDA:ComplexPortal.
DR InterPro; IPR032523; CcmF_C.
DR InterPro; IPR002541; Cyt_c_assembly.
DR InterPro; IPR003567; Cyt_c_biogenesis.
DR InterPro; IPR003568; Cyt_c_biogenesis_CcmF.
DR PANTHER; PTHR43653; PTHR43653; 1.
DR Pfam; PF16327; CcmF_C; 1.
DR Pfam; PF01578; Cytochrom_C_asm; 1.
DR PRINTS; PR01410; CCBIOGENESIS.
DR PRINTS; PR01411; CCMFBIOGNSIS.
DR TIGRFAMs; TIGR00353; nrfE; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cytochrome c-type biogenesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..647
FT /note="Cytochrome c-type biogenesis protein CcmF"
FT /id="PRO_0000201583"
FT TOPO_DOM 1..8
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..93
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..175
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..210
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..235
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..311
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 333..350
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..389
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..447
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..481
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 503..608
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 609..629
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 630..647
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 647 AA; 71389 MW; 162316A1ED156DA8 CRC64;
MMPEIGNGLL CLALGIALLL SVYPLWGVAR GDARMMASSR LFAWLLFMSV AGAFLVLVNA
FVVNDFTVTY VASNSNTQLP VWYRVAATWG AHEGSLLLWV LLMSGWTFAV AIFSQRIPLD
IVARVLAIMG MVSVGFLLFI LFTSNPFSRT LPNFPIEGRD LNPLLQDPGL IFHPPLLYMG
YVGFSVAFAF AIASLLSGRL DSTYARFTRP WTLAAWIFLT LGIVLGSAWA YYELGWGGWW
FWDPVENASF MPWLVGTALM HSLAVTEQRA SFKAWTLLLA ISAFSLCLLG TFLVRSGVLV
SVHAFASDPA RGMFILAFMV LVIGGSLLLF AARGHKVRSR VNNALWSRES LLLANNVLLV
AAMLVVLLGT LLPLVHKQLG LGSISIGEPF FNTMFTWLMV PFALLLGVGP LVRWGRDRPR
KIRNLLIIAF ISTLVLSLLL PWLFESKVVA MTVLGLAMAC WIAVLAIAEA ALRISRGTKT
TFSYWGMVAA HLGLAVTIVG IAFSQNYSVE RDVRMKSGDS VDIHEYRFTF RDVKEVTGPN
WRGGVATIGV TRDGKPETVL YAEKRYYNTA GSMMTEAAID GGITRDLYAA LGEELENGAW
AVRLYYKPFV RWIWAGGLMM ALGGLLCLFD PRYRKRVSPQ KTAPEAV
