CCML_NOSS1
ID CCML_NOSS1 Reviewed; 101 AA.
AC Q8YYI2;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Carboxysome shell vertex protein CcmL {ECO:0000255|HAMAP-Rule:MF_00858};
DE AltName: Full=Carbon dioxide concentrating mechanism protein CcmL {ECO:0000255|HAMAP-Rule:MF_00858};
GN Name=ccmL {ECO:0000255|HAMAP-Rule:MF_00858}; OrderedLocusNames=all0866;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
RN [2] {ECO:0007744|PDB:4N8X}
RP X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS), SUBUNIT, DOMAIN, AND MUTAGENESIS OF
RP GLN-38.
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=24504539; DOI=10.1007/s11120-014-9973-z;
RA Keeling T.J., Samborska B., Demers R.W., Kimber M.S.;
RT "Interactions and structural variability of beta-carboxysomal shell protein
RT CcmL.";
RL Photosyn. Res. 121:125-133(2014).
CC -!- FUNCTION: Probably forms vertices in the carboxysome, a polyhedral
CC inclusion where RuBisCO (ribulose bisphosphate carboxylase, rbcL-rbcS)
CC is sequestered. Has been modeled to induce curvature upon insertion
CC into an otherwise flat hexagonal molecular layer of CcmK subunits.
CC {ECO:0000250|UniProtKB:P72759, ECO:0000255|HAMAP-Rule:MF_00858}.
CC -!- SUBUNIT: Homopentamer (PubMed:24504539). Interacts with full-length
CC CcmM (By similarity). {ECO:0000250|UniProtKB:P72759,
CC ECO:0000269|PubMed:24504539}.
CC -!- SUBCELLULAR LOCATION: Carboxysome {ECO:0000250|UniProtKB:Q03512,
CC ECO:0000255|HAMAP-Rule:MF_00858}. Note=This cyanobacterium makes beta-
CC type carboxysomes. Probably forms vertices in the carboxysome.
CC {ECO:0000305|PubMed:24504539}.
CC -!- DOMAIN: The tight homopentamer forms a pore with an opening of about 5
CC Angstroms in diameter which opens into a wider tunnel at the base of
CC the truncated pyramid. The pore is positively charged.
CC {ECO:0000269|PubMed:24504539}.
CC -!- SIMILARITY: Belongs to the CcmL/EutN family. CcmL subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00858}.
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DR EMBL; BA000019; BAB72823.1; -; Genomic_DNA.
DR PIR; AH1914; AH1914.
DR RefSeq; WP_010995040.1; NZ_RSCN01000006.1.
DR PDB; 4N8X; X-ray; 1.93 A; 1/2/3/4/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z=1-101.
DR PDBsum; 4N8X; -.
DR AlphaFoldDB; Q8YYI2; -.
DR SMR; Q8YYI2; -.
DR STRING; 103690.17130211; -.
DR EnsemblBacteria; BAB72823; BAB72823; BAB72823.
DR GeneID; 58722042; -.
DR KEGG; ana:all0866; -.
DR eggNOG; COG4576; Bacteria.
DR OMA; GAGINEW; -.
DR OrthoDB; 1832681at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0031470; C:carboxysome; IEA:UniProtKB-SubCell.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR CDD; cd01614; EutN_CcmL; 1.
DR Gene3D; 2.40.50.220; -; 1.
DR HAMAP; MF_00858; CcmL; 1.
DR InterPro; IPR046387; CcmL.
DR InterPro; IPR004992; EutN_CcmL.
DR InterPro; IPR036677; EutN_CcmL_sf.
DR PANTHER; PTHR36539; PTHR36539; 1.
DR Pfam; PF03319; EutN_CcmL; 1.
DR SUPFAM; SSF159133; SSF159133; 1.
DR PROSITE; PS51932; BMV; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacterial microcompartment; Carbon dioxide fixation;
KW Carboxysome; Photosynthesis; Reference proteome.
FT CHAIN 1..101
FT /note="Carboxysome shell vertex protein CcmL"
FT /id="PRO_0000451290"
FT DOMAIN 1..84
FT /note="BMV"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00858"
FT MUTAGEN 38
FT /note="Q->A: No change in oligomerization."
FT /evidence="ECO:0000269|PubMed:24504539"
FT STRAND 2..11
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:4N8X"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 24..30
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 36..44
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 55..60
FT /evidence="ECO:0007829|PDB:4N8X"
FT HELIX 61..66
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 76..88
FT /evidence="ECO:0007829|PDB:4N8X"
FT STRAND 91..95
FT /evidence="ECO:0007829|PDB:4N8X"
FT TURN 96..98
FT /evidence="ECO:0007829|PDB:4N8X"
SQ SEQUENCE 101 AA; 11192 MW; A07C2007022E207A CRC64;
MQIAKVRGTV VSTQKDPSLR GVKLLLLQLV DEEGNLLQKY EVAADNSVGA GFDEWVLISR
GSAARQLLGN EQRPVDAAVV AIIDTIHVED RLIYSKKDQY R
