ID   CCMN_SYNY3              Reviewed;         241 AA.
AC   P72757;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=Carboxysome assembly protein CcmN {ECO:0000305};
DE   AltName: Full=Carbon dioxide concentrating mechanism protein CcmN;
GN   Name=ccmN {ECO:0000303|PubMed:17993516}; OrderedLocusNames=sll1032;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [2]
RP   INTERACTION WITH CCMM.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=17993516; DOI=10.1128/jb.01283-07;
RA   Cot S.S., So A.K., Espie G.S.;
RT   "A multiprotein bicarbonate dehydration complex essential to carboxysome
RT   function in cyanobacteria.";
RL   J. Bacteriol. 190:936-945(2008).
CC   -!- FUNCTION: Required for carboxysome formation; the N-terminus interacts
CC       with CcmM which itself binds RuBisCO (ribulose bisphosphate
CC       carboxylase, rbcL-rbcS) (PubMed:17993516). May also contact shell
CC       protein CcmK to help assemble the carboxysome (By similarity).
CC       {ECO:0000250|UniProtKB:P46204, ECO:0000269|PubMed:17993516}.
CC   -!- FUNCTION: Beta-carboxysome assembly initiates when soluble RuBisCO is
CC       condensed into a liquid matrix in a pre-carboxysome by the RbcS-like
CC       domains of probably both forms of CcmM. CcmN interacts with the N-
CC       terminus of full-length CcmM, and then recruits the CcmK major shell
CC       protein via CcmN's encapsulation peptide. Shell formation requires CcmK
CC       proteins and CcmO. CcmL caps the otherwise elongated carboxysome. Once
CC       fully encapsulated carboxysomes are formed, they migrate within the
CC       cell probably via interactions with the cytoskeleton.
CC       {ECO:0000250|UniProtKB:P46204}.
CC   -!- SUBUNIT: Interacts with full-length and the N-terminal 249 residues of
CC       CcmM; a probable CcmM-CcaA-CcmN complex can also be isolated
CC       (PubMed:17993516). Interacts with CcmK (By similarity).
CC       {ECO:0000250|UniProtKB:P46204, ECO:0000269|PubMed:17993516}.
CC   -!- INTERACTION:
CC       P72757; P72758: ccmM; NbExp=3; IntAct=EBI-1609426, EBI-862848;
CC   -!- SUBCELLULAR LOCATION: Carboxysome {ECO:0000305|PubMed:17993516}.
CC       Note=This cyanobacterium makes beta-type carboxysomes.
CC       {ECO:0000305|PubMed:17993516}.
CC   -!- SIMILARITY: Belongs to the CcmN family. {ECO:0000305}.
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DR   EMBL; BA000022; BAA16772.1; -; Genomic_DNA.
DR   PIR; S74620; S74620.
DR   AlphaFoldDB; P72757; -.
DR   SMR; P72757; -.
DR   IntAct; P72757; 10.
DR   STRING; 1148.1651845; -.
DR   PaxDb; P72757; -.
DR   EnsemblBacteria; BAA16772; BAA16772; BAA16772.
DR   KEGG; syn:sll1032; -.
DR   eggNOG; COG0663; Bacteria.
DR   InParanoid; P72757; -.
DR   OMA; TLFPHRQ; -.
DR   PhylomeDB; P72757; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0031470; C:carboxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0043886; F:structural constituent of carboxysome; IEA:UniProt.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   SUPFAM; SSF51161; SSF51161; 1.
PE   1: Evidence at protein level;
KW   Bacterial microcompartment; Carbon dioxide fixation; Carboxysome;
KW   Photosynthesis; Reference proteome.
FT   CHAIN           1..241
FT                   /note="Carboxysome assembly protein CcmN"
FT                   /id="PRO_0000451245"
FT   REGION          123..206
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           219..241
FT                   /note="Encapsulation peptide"
FT                   /evidence="ECO:0000250|UniProtKB:P46204"
FT   COMPBIAS        123..142
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        159..206
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   241 AA;  25057 MW;  2555A88B4EB8B9D4 CRC64;
     MQLPPVHSVS LSEYFVSGNV IIHETAVIAP GVILEAAPDC QITIEAGVCI GLGSVISAHA
     GDVKIQEQTA IAPGCLVIGP VTIGATACLG SRSTVFQQDI DAQVLIPPGS LLMNRVADVQ
     TVGASSPTTD SVTEKKSPST ANPIAPIPSP WDNEPPAKGT DSPSDQAKES IARQSRPSTA
     EAAEQISSNR SPGESTPTAP TVVTTAPLVS EEVQEKPPVV GQVYINQLLL TLFPERRYFS
     S