1A_AMVLE
ID 1A_AMVLE Reviewed; 1126 AA.
AC P03589;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Replication protein 1a;
DE Includes:
DE RecName: Full=ATP-dependent helicase;
DE EC=3.6.4.-;
DE Includes:
DE RecName: Full=Methyltransferase;
DE EC=2.1.1.-;
GN ORFNames=ORF1a;
OS Alfalfa mosaic virus (strain 425 / isolate Leiden).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Bromoviridae; Alfamovirus.
OX NCBI_TaxID=12322;
OH NCBI_TaxID=4045; Apium graveolens (Celery).
OH NCBI_TaxID=83862; Astragalus glycyphyllos (Wild liquorice).
OH NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH NCBI_TaxID=41386; Caryopteris incana.
OH NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH NCBI_TaxID=35936; Lablab purpureus (Hyacinth bean) (Dolichos lablab).
OH NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH NCBI_TaxID=3864; Lens culinaris (Lentil) (Cicer lens).
OH NCBI_TaxID=3869; Lupinus.
OH NCBI_TaxID=145753; Malva parviflora (Little mallow) (Cheeseweed mallow).
OH NCBI_TaxID=3879; Medicago sativa (Alfalfa).
OH NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH NCBI_TaxID=23113; Philadelphus.
OH NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH NCBI_TaxID=157662; Teramnus repens.
OH NCBI_TaxID=60916; Trifolium incarnatum (Crimson clover).
OH NCBI_TaxID=85293; Viburnum opulus (High-bush cranberry).
OH NCBI_TaxID=3916; Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OH NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=6298738; DOI=10.1093/nar/11.5.1253;
RA Cornelissen B.J.C., Brederode F.T., Moormann R.J.M., Bol J.F.;
RT "Complete nucleotide sequence of alfalfa mosaic virus RNA 1.";
RL Nucleic Acids Res. 11:1253-1265(1983).
CC -!- FUNCTION: Involved in the virus replication. Contains a helicase domain
CC and a methyltransferase domain. The methyltransferase domain is
CC probably involved in viral RNA capping. Involved in the formation of ER
CC membrane spherular invaginations in which RNA replication complexes
CC form (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with RNA-directed RNA polymerase 2a. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the bromoviridae replication protein 1a family.
CC {ECO:0000305}.
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DR EMBL; L00163; AAA46289.1; -; Genomic_RNA.
DR PIR; A04197; WMFM12.
DR RefSeq; NP_041192.1; NC_001495.1.
DR SMR; P03589; -.
DR PRIDE; P03589; -.
DR GeneID; 962667; -.
DR KEGG; vg:962667; -.
DR Proteomes; UP000000358; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Host endoplasmic reticulum; Host membrane;
KW Hydrolase; Membrane; Methyltransferase; Nucleotide-binding;
KW Reference proteome; Transferase.
FT CHAIN 1..1126
FT /note="Replication protein 1a"
FT /id="PRO_0000083254"
FT DOMAIN 90..278
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 806..963
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 964..1125
FT /note="(+)RNA virus helicase C-terminal"
FT REGION 69..406
FT /note="Methyltransferase"
FT REGION 834..1094
FT /note="ATP-dependent helicase"
FT BINDING 838..845
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1126 AA; 125828 MW; BF5A8019B47D4CBF CRC64;
MNADAQSTDA SLSMREPLSH ASIQEMLRRV VEKQAADDTT AIGKVFSEAG RAYAQDALPS
DKGEVLKISF SLDATQQNIL RANFPGRRTV FSNSSSSSHC FAAAHRLLET DFVYRCFGNT
VDSIIDLGGN FVSHMKVKRH NVHCCCPILD ARDGARLTER ILSLKSYVRK HPEIVGEADY
CMDTFQKCSR RADYAFAIHS TSDLDVGELA CSLDQKGVMK FICTMMVDAD MLIHNEGEIP
NFNVRWEIDR KKDLIHFDFI DEPNLGYSHR FSLLKHYLTY NAVDLGHAAY RIERKQDFGG
VMVIDLTYSL GFVPKMPHSN GRSCAWYNRV KGQMVVHTVN EGYYHHSYQT AVRRKVLVDK
KVLTRVTEVA FRQFRPNADA HSAIQSIATM LSSSTNHTII GGVTLISGKP LSPDDYIPVA
TTIYYRVKKL YNAIPEMLSL LDKGERLSTD AVLKGSEGPM WYSGPTFLSA LDKVNVPGDF
VAKALLSLPK RDLKSLFSRS ATSHSERTPV RDESPIRCTD GVFYPIRMLL KCLGSDKFES
VTITDPRSNT ETTVDLYQSF QKKIETVFSF ILGKIDGPSP LISDPVYFQS LEDVYYAEWH
QGNAIDASNY ARTLLDDIRK QKEESLKAKA KEVEDAQKLN RAILQVHAYL EAHPDGGKIE
GLGLSSQFIA KIPELAIPTP KPLPEFEKNA ETGEILRINP HSDAILEAID YLKSTSANSI
ITLNKLGDHC QWTTKGLDVV WAGDDKRRAF IPKKNTWVGP TARSYPLAKY ERAMSKDGYV
TLRWDGEVLD ANCVRSLSQY EIVFVDQSCV FASAEAIIPS LEKALGLEAH FSVTIVDGVA
GCGKTTNIKQ IARSSGRDVD LILTSNRSSA DELKETIDCS PLTKLHYIRT CDSYLMSASA
VKAQRLIFDE CFLQHAGLVY AAATLAGCSE VIGFGDTEQI PFVSRNPSFV FRHHKLTGKV
ERKLITWRSP ADATYCLEKY FYKNKKPVKT NSRVLRSIEV VPINSPVSVE RNTNALYLCH
TQAEKAVLKA QTHLKGCDNI FTTHEAQGKT FDNVYFCRLT RTSTSLATGR DPINGPCNGL
VALSRHKKTF KYFTIAHDSD DVIYNACRDA GNTDDSILAR SYNHNF
