CCNE_CAEEL
ID CCNE_CAEEL Reviewed; 524 AA.
AC O01501; O77098; Q86FL2;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=G1/S-specific cyclin-E;
GN Name=cye-1 {ECO:0000312|WormBase:C37A2.4a};
GN ORFNames=C37A2.4 {ECO:0000312|WormBase:C37A2.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12606285; DOI=10.1016/s0012-1606(02)00032-5;
RA Brodigan T.M., Liu J., Park M., Kipreos E.T., Krause M.;
RT "Cyclin E expression during development in Caenorhabditis elegans.";
RL Dev. Biol. 254:102-115(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10952902; DOI=10.1242/dev.127.18.4049;
RA Fay D.S., Han M.;
RT "Mutations in cye-1, a Caenorhabditis elegans cyclin E homolog, reveal
RT coordination between cell-cycle control and vulval development.";
RL Development 127:4049-4060(2000).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=17115027; DOI=10.1038/ncb1511;
RA Cowan C.R., Hyman A.A.;
RT "Cyclin E-Cdk2 temporally regulates centrosome assembly and establishment
RT of polarity in Caenorhabditis elegans embryos.";
RL Nat. Cell Biol. 8:1441-1447(2006).
RN [5]
RP FUNCTION.
RX PubMed=17466069; DOI=10.1186/1471-213x-7-38;
RA Ouellet J., Roy R.;
RT "The lin-35/Rb and RNAi pathways cooperate to regulate a key cell cycle
RT transition in C. elegans.";
RL BMC Dev. Biol. 7:38-38(2007).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17476329; DOI=10.1371/journal.pone.0000407;
RA Fujita M., Takeshita H., Sawa H.;
RT "Cyclin E and CDK2 repress the terminal differentiation of quiescent cells
RT after asymmetric division in C. elegans.";
RL PLoS ONE 2:E407-E407(2007).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=19758560; DOI=10.1016/j.devcel.2009.08.003;
RA Biedermann B., Wright J., Senften M., Kalchhauser I., Sarathy G., Lee M.H.,
RA Ciosk R.;
RT "Translational repression of cyclin E prevents precocious mitosis and
RT embryonic gene activation during C. elegans meiosis.";
RL Dev. Cell 17:355-364(2009).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20005870; DOI=10.1016/j.ydbio.2009.12.005;
RA Inoue T., Sternberg P.W.;
RT "C. elegans BED domain transcription factor BED-3 controls lineage-specific
RT cell proliferation during organogenesis.";
RL Dev. Biol. 338:226-236(2010).
RN [9]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=21558371; DOI=10.1242/dev.059535;
RA Fox P.M., Vought V.E., Hanazawa M., Lee M.H., Maine E.M., Schedl T.;
RT "Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle
RT progression in the C. elegans germline.";
RL Development 138:2223-2234(2011).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21455289; DOI=10.1371/journal.pgen.1001348;
RA Jeong J., Verheyden J.M., Kimble J.;
RT "Cyclin E and Cdk2 control GLD-1, the mitosis/meiosis decision, and
RT germline stem cells in Caenorhabditis elegans.";
RL PLoS Genet. 7:E1001348-E1001348(2011).
CC -!- FUNCTION: Essential for the control of the cell cycle at the G1/S
CC (start) transition (PubMed:12606285). In association with cdk-2,
CC regulates proliferation, quiescent state and cell fate during the
CC development of several cell lineages (PubMed:10952902, PubMed:17476329,
CC PubMed:21558371, PubMed:21455289). In the embryo, initiates the
CC establishment of cell polarity through the recruitment of the
CC centrosomal proteins spd-2 and spd-5 during prophase (PubMed:17115027).
CC During the development of the vulva, controls the onset of vulval cell
CC terminal differentiation by controlling the duration of G1 phase
CC (PubMed:10952902, PubMed:20005870). During hypoderm development at
CC early larval stages, controls syncytial fate of seam cell daughter
CC cells (PubMed:17476329). Involved in the progression of cell division
CC in the intestinal lineage in larvae, and in particular in
CC endoreplication, a specific growth pathway in the intestinal
CC epithelium, required for feeding and gut development in growing larvae
CC (PubMed:17466069). By controlling the activity of translational
CC repressor gld-1, regulates the pool of germline stem cells and the size
CC of the mitotic zone by preventing entry into meiosis (PubMed:21455289).
CC In addition, repression of expression by gld-1 prevents mitosis re-
CC entry in meiotic germline cells (PubMed:19758560).
CC {ECO:0000269|PubMed:10952902, ECO:0000269|PubMed:12606285,
CC ECO:0000269|PubMed:17115027, ECO:0000269|PubMed:17466069,
CC ECO:0000269|PubMed:17476329, ECO:0000269|PubMed:19758560,
CC ECO:0000269|PubMed:20005870, ECO:0000269|PubMed:21455289,
CC ECO:0000269|PubMed:21558371}.
CC -!- SUBUNIT: Interacts with a member of the CDK2/CDK protein kinases to
CC form a serine/threonine kinase holoenzyme complex. The cyclin subunit
CC imparts substrate specificity to the complex (By similarity).
CC {ECO:0000250|UniProtKB:P24864}.
CC -!- INTERACTION:
CC O01501; O61847: cdk-2; NbExp=3; IntAct=EBI-6499833, EBI-14063070;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12606285}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome, centriole
CC {ECO:0000269|PubMed:17115027}. Note=Co-localizes with cdk-2 in the
CC sperm centrioles before the first embryonic mitosis and then to the
CC male and female nuclei upon entry into mitosis.
CC {ECO:0000269|PubMed:12606285, ECO:0000269|PubMed:17115027}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000269|PubMed:12606285};
CC IsoId=O01501-1; Sequence=Displayed;
CC Name=b {ECO:0000269|PubMed:12606285};
CC IsoId=O01501-2; Sequence=VSP_007909;
CC -!- TISSUE SPECIFICITY: Expressed dynamically in proliferating cells
CC throughout development. Detectable in larval blast cells undergoing
CC active proliferation that give rise to all tissue types, including
CC germline, intestine, hypodermis, neurons, and muscle.
CC {ECO:0000269|PubMed:12606285}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Ubiquitous embryonic pattern of expression declines during
CC embryogenesis and disappears from most cells in comma-stage embryos
CC coincident with the completion of the majority of embryonic cell
CC divisions. Expression levels drop and become restricted and dynamic
CC during postembryonic development (PubMed:12606285). During the
CC development of distal tip cells, expressed asymmetrically between the
CC daughters of the Z1.a and Z4.p cells; asymmetric expression is
CC regulated by wrm-1, a component of the Wnt/MAPK pathway
CC (PubMed:17476329). In the gonads, expression is restricted to the
CC proliferating distal germline cells (PubMed:12606285, PubMed:21558371).
CC In germline cells entering meiosis, expression is repressed by gld-1
CC (PubMed:21455289, PubMed:19758560). {ECO:0000269|PubMed:12606285,
CC ECO:0000269|PubMed:17476329, ECO:0000269|PubMed:19758560,
CC ECO:0000269|PubMed:21455289, ECO:0000269|PubMed:21558371}.
CC -!- DISRUPTION PHENOTYPE: Loss of cell division in vulval lineages as a
CC result of lengthened intervals between cell divisons (PubMed:20005870).
CC RNAi-mediated knockdown results in arrest prior to the 100-cell
CC embryonic stage (PubMed:10952902). Depending on the knockdown, some
CC animals reach adult age (PubMed:17476329). In the 1-cell embryo,
CC persistent ruffling throughout the embryo cortex and mislocalization of
CC par-2, which remains cytoplasmic, and par-6, which is distributed
CC throughout the cortex. In 50 percent of embryos, the first division is
CC symmetric. Adult mutants are sterile due to a lack of sperm and egg
CC production (PubMed:17115027). In mutants and knockdown animals,
CC production of 2 additional distal tip cells (DTC) is often associated
CC with the production of an extra gonad (PubMed:17476329). In addition,
CC gonads of knockdown animals have an abnormal mitotic zone characterized
CC by an enlargement of the distal germ cell nuclei, a reduction in the
CC number of mitotic germ cells, a reduction in the mitotic region length
CC and an abnormal expression of gld-1 (PubMed:21455289).
CC {ECO:0000269|PubMed:10952902, ECO:0000269|PubMed:17115027,
CC ECO:0000269|PubMed:17476329, ECO:0000269|PubMed:20005870,
CC ECO:0000269|PubMed:21455289}.
CC -!- SIMILARITY: Belongs to the cyclin family. Cyclin E subfamily.
CC {ECO:0000305}.
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DR EMBL; AF520616; AAM78547.1; -; mRNA.
DR EMBL; AF058331; AAC63505.1; -; mRNA.
DR EMBL; FO080805; CCD66921.1; -; Genomic_DNA.
DR EMBL; FO080805; CCD66922.1; -; Genomic_DNA.
DR PIR; T43050; T43050.
DR RefSeq; NP_001021027.1; NM_001025856.3. [O01501-1]
DR RefSeq; NP_001021028.1; NM_001025857.2. [O01501-2]
DR AlphaFoldDB; O01501; -.
DR SMR; O01501; -.
DR BioGRID; 37847; 22.
DR ComplexPortal; CPX-1127; Cyclin cye-1-cdk2 complex.
DR IntAct; O01501; 7.
DR STRING; 6239.C37A2.4a; -.
DR EPD; O01501; -.
DR PaxDb; O01501; -.
DR PeptideAtlas; O01501; -.
DR EnsemblMetazoa; C37A2.4a.1; C37A2.4a.1; WBGene00000871. [O01501-1]
DR EnsemblMetazoa; C37A2.4b.1; C37A2.4b.1; WBGene00000871. [O01501-2]
DR GeneID; 172399; -.
DR KEGG; cel:CELE_C37A2.4; -.
DR UCSC; C37A2.4a; c. elegans. [O01501-1]
DR CTD; 172399; -.
DR WormBase; C37A2.4a; CE24832; WBGene00000871; cye-1. [O01501-1]
DR WormBase; C37A2.4b; CE33761; WBGene00000871; cye-1. [O01501-2]
DR eggNOG; KOG0655; Eukaryota.
DR GeneTree; ENSGT00940000169122; -.
DR InParanoid; O01501; -.
DR OMA; SEESHEM; -.
DR OrthoDB; 993640at2759; -.
DR PhylomeDB; O01501; -.
DR Reactome; R-CEL-1538133; G0 and Early G1.
DR Reactome; R-CEL-187577; SCF(Skp2)-mediated degradation of p27/p21.
DR Reactome; R-CEL-2559586; DNA Damage/Telomere Stress Induced Senescence.
DR Reactome; R-CEL-6804116; TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest.
DR Reactome; R-CEL-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR Reactome; R-CEL-69200; Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
DR Reactome; R-CEL-69202; Cyclin E associated events during G1/S transition.
DR Reactome; R-CEL-69563; p53-Dependent G1 DNA Damage Response.
DR Reactome; R-CEL-9706019; RHOBTB3 ATPase cycle.
DR PRO; PR:O01501; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00000871; Expressed in adult organism and 4 other tissues.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0097134; C:cyclin E1-CDK2 complex; IPI:ComplexPortal.
DR GO; GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; ISS:WormBase.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0016538; F:cyclin-dependent protein serine/threonine kinase regulator activity; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0000082; P:G1/S transition of mitotic cell cycle; IDA:UniProtKB.
DR GO; GO:0007281; P:germ cell development; IMP:WormBase.
DR GO; GO:0051729; P:germline cell cycle switching, mitotic to meiotic cell cycle; IMP:WormBase.
DR GO; GO:0008406; P:gonad development; IGI:UniProtKB.
DR GO; GO:0048815; P:hermaphrodite genitalia morphogenesis; IMP:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0044772; P:mitotic cell cycle phase transition; IBA:GO_Central.
DR GO; GO:0051782; P:negative regulation of cell division; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:WormBase.
DR GO; GO:0045931; P:positive regulation of mitotic cell cycle; IGI:WormBase.
DR GO; GO:1904781; P:positive regulation of protein localization to centrosome; IGI:UniProtKB.
DR GO; GO:0040026; P:positive regulation of vulval development; IMP:WormBase.
DR GO; GO:0009791; P:post-embryonic development; IMP:WormBase.
DR GO; GO:0010608; P:post-transcriptional regulation of gene expression; IMP:WormBase.
DR GO; GO:0006468; P:protein phosphorylation; IMP:WormBase.
DR GO; GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0051445; P:regulation of meiotic cell cycle; IMP:UniProtKB.
DR GO; GO:0007088; P:regulation of mitotic nuclear division; IDA:UniProtKB.
DR GO; GO:1904776; P:regulation of protein localization to cell cortex; IMP:UniProtKB.
DR CDD; cd00043; CYCLIN; 1.
DR InterPro; IPR039361; Cyclin.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR006671; Cyclin_N.
DR PANTHER; PTHR10177; PTHR10177; 1.
DR Pfam; PF00134; Cyclin_N; 1.
DR SMART; SM00385; CYCLIN; 1.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS00292; CYCLINS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Cyclin; Cytoplasm;
KW Cytoskeleton; Meiosis; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..524
FT /note="G1/S-specific cyclin-E"
FT /id="PRO_0000080458"
FT REGION 1..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..104
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..121
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 100..102
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000303|PubMed:12606285"
FT /id="VSP_007909"
SQ SEQUENCE 524 AA; 60586 MW; 5B16BA7E70AA5F15 CRC64;
MAGRKSSRTA ERVPTTQKPE RKSAILSPHD ELRERLLETA IDMKENIPQR NTRNSSVGSQ
KSDCSETRKR RSTKEGPAAK RHSGEKHRNG SREDSLEYIS EYSDDREVGS SSSQSSRTRG
QPLPAMPEEE EVFDKSSSSD NLAESEESHE MVRLEERQDI EEEIEDDFDD EEEDVVNDKE
EYEEIESEDE DDYPVQNEGF AVTKRLMNDE HMVTAPTFLS TAKCDGIGSP TKVWSLMVKR
DEIPRATRFL LGNHPDMDDE KRRILIDWMM EVCESEKLHR ETFHLAVDYV DRYLESSNVE
CSTDNFQLVG TAALFIAAKY EEIYPPKCID FAHLTDSAFT CDNIRTMEVL IVKYIGWSLG
PITSIQWLST YLQLLGTGKK NKSDHYEEQN MYVPELLRSE YLEMCKILDF LLFEIDSFTF
SYRTIAAAVL FVNYEPTCAV EKATGFMQAQ LEKVIEYVEP VCRAFAKQRQ LLDDVIPKHE
SIKSDDSHNI QVYVKRSSME PIVKSERERI QHLKARRLHP QRLF
