CCNT_DROME
ID CCNT_DROME Reviewed; 1097 AA.
AC O96433; Q9VVI6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Cyclin-T;
GN Name=CycT; ORFNames=CG6292;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RX PubMed=9593731; DOI=10.1074/jbc.273.22.13855;
RA Peng J.-M., Marshall N.F., Price D.H.;
RT "Identification of a cyclin subunit required for the function of Drosophila
RT P-TEFb.";
RL J. Biol. Chem. 273:13855-13860(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-916, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [5]
RP IDENTIFICATION IN THE SEC COMPLEX.
RX PubMed=22195968; DOI=10.1016/j.molcel.2011.12.008;
RA Smith E.R., Lin C., Garrett A.S., Thornton J., Mohaghegh N., Hu D.,
RA Jackson J., Saraf A., Swanson S.K., Seidel C., Florens L., Washburn M.P.,
RA Eissenberg J.C., Shilatifard A.;
RT "The little elongation complex regulates small nuclear RNA transcription.";
RL Mol. Cell 44:954-965(2011).
CC -!- FUNCTION: Regulatory subunit of the cyclin-dependent kinase pair
CC (CDK9/cyclin T) complex, also called positive transcription elongation
CC factor B (P-TEFb), which is proposed to facilitate the transition from
CC abortive to production elongation by phosphorylating the CTD (carboxy-
CC terminal domain) of the large subunit of RNA polymerase II (RNAP II).
CC -!- SUBUNIT: Component of the super elongation complex (SEC), at least
CC composed of Ell, Cdk9, cyclin-T (CycT), lilli and ear. Associates with
CC CDK9 to form P-TEFb. {ECO:0000269|PubMed:22195968}.
CC -!- INTERACTION:
CC O96433; O17432: Cdk9; NbExp=2; IntAct=EBI-191577, EBI-100284;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=O96433-1; Sequence=Displayed;
CC Name=A;
CC IsoId=O96433-2; Sequence=VSP_010290;
CC -!- SIMILARITY: Belongs to the cyclin family. Cyclin C subfamily.
CC {ECO:0000305}.
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DR EMBL; AF051933; AAC73052.1; -; mRNA.
DR EMBL; AE014296; AAF49325.1; -; Genomic_DNA.
DR EMBL; AE014296; AAS64974.1; -; Genomic_DNA.
DR PIR; T13033; T13033.
DR RefSeq; NP_001261992.1; NM_001275063.2. [O96433-1]
DR RefSeq; NP_001261993.1; NM_001275064.2. [O96433-2]
DR RefSeq; NP_524127.2; NM_079403.4. [O96433-1]
DR RefSeq; NP_996117.1; NM_206395.3. [O96433-2]
DR AlphaFoldDB; O96433; -.
DR SMR; O96433; -.
DR BioGRID; 65249; 11.
DR DIP; DIP-29892N; -.
DR IntAct; O96433; 4.
DR STRING; 7227.FBpp0305335; -.
DR iPTMnet; O96433; -.
DR PaxDb; O96433; -.
DR EnsemblMetazoa; FBtr0075205; FBpp0074968; FBgn0025455. [O96433-1]
DR EnsemblMetazoa; FBtr0075206; FBpp0089275; FBgn0025455. [O96433-2]
DR EnsemblMetazoa; FBtr0333124; FBpp0305335; FBgn0025455. [O96433-1]
DR EnsemblMetazoa; FBtr0333125; FBpp0305336; FBgn0025455. [O96433-2]
DR GeneID; 39961; -.
DR KEGG; dme:Dmel_CG6292; -.
DR CTD; 13067; -.
DR FlyBase; FBgn0025455; CycT.
DR VEuPathDB; VectorBase:FBgn0025455; -.
DR eggNOG; KOG0834; Eukaryota.
DR GeneTree; ENSGT00940000170249; -.
DR HOGENOM; CLU_283750_0_0_1; -.
DR InParanoid; O96433; -.
DR PhylomeDB; O96433; -.
DR Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-DME-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DME-6807505; RNA polymerase II transcribes snRNA genes.
DR Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DME-9018519; Estrogen-dependent gene expression.
DR SignaLink; O96433; -.
DR BioGRID-ORCS; 39961; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 39961; -.
DR PRO; PR:O96433; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0025455; Expressed in egg chamber and 30 other tissues.
DR ExpressionAtlas; O96433; baseline and differential.
DR Genevisible; O96433; DM.
DR GO; GO:0008024; C:cyclin/CDK positive transcription elongation factor complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0070691; C:P-TEFb complex; IPI:FlyBase.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0005703; C:polytene chromosome puff; IDA:FlyBase.
DR GO; GO:0032783; C:super elongation complex; IPI:FlyBase.
DR GO; GO:0008023; C:transcription elongation factor complex; IDA:UniProtKB.
DR GO; GO:0061575; F:cyclin-dependent protein serine/threonine kinase activator activity; IBA:GO_Central.
DR GO; GO:0016538; F:cyclin-dependent protein serine/threonine kinase regulator activity; IDA:FlyBase.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IDA:FlyBase.
DR GO; GO:0032786; P:positive regulation of DNA-templated transcription, elongation; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IDA:FlyBase.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:FlyBase.
DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase.
DR CDD; cd00043; CYCLIN; 1.
DR InterPro; IPR028863; CCNT1.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR043198; Cyclin/Ssn8.
DR InterPro; IPR006671; Cyclin_N.
DR PANTHER; PTHR10026; PTHR10026; 1.
DR PANTHER; PTHR10026:SF131; PTHR10026:SF131; 1.
DR Pfam; PF00134; Cyclin_N; 1.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cyclin; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1097
FT /note="Cyclin-T"
FT /id="PRO_0000080498"
FT REGION 319..782
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 804..936
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 985..1097
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..351
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..526
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 578..621
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 655..682
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 722..742
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 761..782
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 857..872
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 884..910
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1011..1030
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 916
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..213
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_010290"
FT CONFLICT 55
FT /note="S -> L (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="D -> E (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 406
FT /note="T -> P (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 440
FT /note="R -> H (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 575
FT /note="P -> H (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 639
FT /note="P -> L (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 686
FT /note="V -> M (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
FT CONFLICT 901
FT /note="K -> R (in Ref. 1; AAC73052)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1097 AA; 118269 MW; 0E0E71B8A674FC5B CRC64;
MSLLATPMPQ AATASSSSSA SAAASASGIP ITANNNLPFE KDKIWYFSND QLANSPSRRC
GIKGDDELQY RQMTAYLIQE MGQRLQVSQL CINTAIVYMH RFYAFHSFTH FHRNSMASAS
LFLAAKVEEQ PRKLEHVIRA ANKCLPPTTE QNYAELAQEL VFNENVLLQT LGFDVAIDHP
HTHVVRTCQL VKACKDLAQT SYFLASNSLH LTSMCLQYRP TVVACFCIYL ACKWSRWEIP
QSTEGKHWFY YVDKTVSLDL LKQLTDEFIA IYEKSPARLK SKLNSIKAIA QGASNRTANS
KDKPKEDWKI TEMMKGYHSN ITTPPELLNG NDSRDRDRDR ERERERERDP SSLLPPPAMV
PQQRRQDGGH QRSSSVSGVP GSSSSSSSSS HKMPNYPGGM PPDAHTDHKS KQPGYNNRMP
SSHQRSSSSG LGSSGSGSQR SSSSSSSSSQ QPGRPSMPVD YHKSSRGMPP VGVGMPPHGS
HKMTSGSKPQ QPQQQPVPHP SASNSSASGM SSKDKSQSNK MYPNAPPPYS NSAPQNPLMS
RGGYPGASNG SQPPPPAGYG GHRSKSGSTV HGMPPFEQQL PYSQSQSYGH MQQQPVPQSQ
QQQMPPEASQ HSLQSKNSLF SPEWPDIKKE PMSQSQPQPF NGLLPPPAPP GHDYKLNSHP
RDKESPKKER LTPTKKDKHR PVMPPVGSGN SSSGSGSSKP MLPPHKKQIP HGGDLLTNPG
ESGSLKRPNE ISGSQYGLNK LDEIDNSNMP REKLRKLDTT TGLPTYPNYE EKHTPLNMSN
GIETTPDLVR SLLKESLCPS NASLLKPDAL TMPGLKPPAE LLEPMPAPAT IKKEQGITPM
TSLASGPAPM DLEVPTKQAG EIKEESSSKS EKKKKKDKHK HKEKDKSKDK TEKEERKKHK
KDKQKDRSGS GGSKDSSLPN EPLKMVIKNP NGSLQAGASA PIKLKISKNK VEPNNYSAAA
GLPGAIGYGL PPTTATTTSA SIGAAAPVLP PYGAGGGGYS SSGGSSSGGS SKKKHSDRDR
DKESKKNKSQ DYAKYNGAGG GIFNPLGGAG AAPNMSGGMG APMSTAVPPS MLLAPTGAVP
PSAAGLAPPP MPVYNKK
