CCPN_BACSU
ID CCPN_BACSU Reviewed; 212 AA.
AC O34994;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Transcriptional repressor CcpN;
DE AltName: Full=Catabolite control protein N;
DE AltName: Full=Control catabolite protein of gluconeogenesis;
GN Name=ccpN; Synonyms=yqzB; OrderedLocusNames=BSU25250;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP FUNCTION IN GLUCONEOGENESIS REGULATION, INDUCTION, DNA-BINDING, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=15720552; DOI=10.1111/j.1365-2958.2005.04473.x;
RA Servant P., Le Coq D., Aymerich S.;
RT "CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression
RT of Bacillus subtilis gluconeogenic genes.";
RL Mol. Microbiol. 55:1435-1451(2005).
CC -!- FUNCTION: Transcription repressor that binds to the promoter of gapB
CC and pckA genes, preventing their expression. Acts as a regulator for
CC catabolite repression of gluconeogenic genes.
CC {ECO:0000269|PubMed:15720552}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:15720552}.
CC -!- DISRUPTION PHENOTYPE: Relieves catabolite repression of gapB and pckA
CC transcription. Disruption is epistatic over a yqfL deletion.
CC {ECO:0000269|PubMed:15720552}.
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DR EMBL; AL009126; CAB14454.1; -; Genomic_DNA.
DR PIR; A69969; A69969.
DR RefSeq; NP_390403.1; NC_000964.3.
DR RefSeq; WP_003237058.1; NZ_JNCM01000036.1.
DR PDB; 3FV6; X-ray; 1.95 A; A/B=63-212.
DR PDB; 3FWR; X-ray; 2.45 A; A/B=63-212.
DR PDB; 3FWS; X-ray; 2.03 A; A/B=63-212.
DR PDBsum; 3FV6; -.
DR PDBsum; 3FWR; -.
DR PDBsum; 3FWS; -.
DR AlphaFoldDB; O34994; -.
DR SMR; O34994; -.
DR IntAct; O34994; 2.
DR STRING; 224308.BSU25250; -.
DR PaxDb; O34994; -.
DR PRIDE; O34994; -.
DR EnsemblBacteria; CAB14454; CAB14454; BSU_25250.
DR GeneID; 937886; -.
DR KEGG; bsu:BSU25250; -.
DR PATRIC; fig|224308.179.peg.2744; -.
DR eggNOG; COG0517; Bacteria.
DR InParanoid; O34994; -.
DR OMA; SVIMTRM; -.
DR PhylomeDB; O34994; -.
DR BioCyc; BSUB:BSU25250-MON; -.
DR EvolutionaryTrace; O34994; -.
DR PRO; PR:O34994; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0045013; P:carbon catabolite repression of transcription; IMP:CACAO.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.10.580.10; -; 1.
DR InterPro; IPR000644; CBS_dom.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR013196; HTH_11.
DR InterPro; IPR016842; UCP026546_HTH-CBS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00571; CBS; 2.
DR Pfam; PF08279; HTH_11; 1.
DR PIRSF; PIRSF026546; UCP026546_CBS_YqzB; 1.
DR SMART; SM00116; CBS; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF54631; SSF54631; 1.
DR PROSITE; PS51371; CBS; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..212
FT /note="Transcriptional repressor CcpN"
FT /id="PRO_0000360044"
FT DOMAIN 6..70
FT /note="HTH deoR-type"
FT DOMAIN 83..139
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 148..211
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DNA_BIND 23..42
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT HELIX 69..75
FT /evidence="ECO:0007829|PDB:3FV6"
FT HELIX 80..82
FT /evidence="ECO:0007829|PDB:3FV6"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:3FV6"
FT HELIX 96..106
FT /evidence="ECO:0007829|PDB:3FV6"
FT STRAND 109..114
FT /evidence="ECO:0007829|PDB:3FV6"
FT STRAND 120..125
FT /evidence="ECO:0007829|PDB:3FV6"
FT HELIX 126..133
FT /evidence="ECO:0007829|PDB:3FV6"
FT TURN 139..141
FT /evidence="ECO:0007829|PDB:3FV6"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:3FV6"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:3FV6"
FT HELIX 163..173
FT /evidence="ECO:0007829|PDB:3FV6"
FT STRAND 176..183
FT /evidence="ECO:0007829|PDB:3FV6"
FT STRAND 185..195
FT /evidence="ECO:0007829|PDB:3FV6"
FT HELIX 196..207
FT /evidence="ECO:0007829|PDB:3FV6"
SQ SEQUENCE 212 AA; 23870 MW; E001269F2BB93F9E CRC64;
MSTIELNKRQ EHILQIVKEN GPITGEHIAE KLNLTRATLR PDLAILTMSG FLEARPRVGY
FYTGKTGTQL LADKLKKLQV KDFQSIPVVI HENVSVYDAI CTMFLEDVGT LFVVDRDAVL
VGVLSRKDLL RASIGQQELT SVPVHIIMTR MPNITVCRRE DYVMDIAKHL IEKQIDALPV
IKDTDKGFEV IGRVTKTNMT KILVSLSENE IL
