CCR4B_ARATH
ID CCR4B_ARATH Reviewed; 603 AA.
AC Q9M2F8;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Carbon catabolite repressor protein 4 homolog 2;
DE Short=CCR4 homolog 2;
DE EC=3.1.13.4;
GN Name=CCR4-2; OrderedLocusNames=At3g58580; ORFNames=F14P22.170;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as catalytic component of the CCR4-NOT core complex,
CC which in the nucleus seems to be a general transcription factor, and in
CC the cytoplasm the major mRNA deadenylase involved in mRNA turnover.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Component of the CCR4-NOT complex, at least composed of CRR4
CC and CAF1 proteins. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CCR4/nocturin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK226830; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAB68196.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL137082; CAB68196.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79801.1; -; Genomic_DNA.
DR EMBL; AK226830; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T45678; T45678.
DR RefSeq; NP_191417.2; NM_115720.3.
DR AlphaFoldDB; Q9M2F8; -.
DR SMR; Q9M2F8; -.
DR STRING; 3702.AT3G58580.1; -.
DR iPTMnet; Q9M2F8; -.
DR PaxDb; Q9M2F8; -.
DR PRIDE; Q9M2F8; -.
DR ProteomicsDB; 223894; -.
DR EnsemblPlants; AT3G58580.1; AT3G58580.1; AT3G58580.
DR GeneID; 825027; -.
DR Gramene; AT3G58580.1; AT3G58580.1; AT3G58580.
DR KEGG; ath:AT3G58580; -.
DR Araport; AT3G58580; -.
DR TAIR; locus:2076446; AT3G58580.
DR eggNOG; KOG0620; Eukaryota.
DR HOGENOM; CLU_016428_5_0_1; -.
DR InParanoid; Q9M2F8; -.
DR OMA; RTIDGCA; -.
DR OrthoDB; 724242at2759; -.
DR PhylomeDB; Q9M2F8; -.
DR PRO; PR:Q9M2F8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M2F8; baseline and differential.
DR Genevisible; Q9M2F8; AT.
DR GO; GO:0030014; C:CCR4-NOT complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004535; F:poly(A)-specific ribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR Gene3D; 3.60.10.10; -; 1.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR Pfam; PF03372; Exo_endo_phos; 1.
DR SUPFAM; SSF56219; SSF56219; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Nucleus; Reference proteome; Repeat; RNA-binding; Transcription;
KW Transcription regulation.
FT CHAIN 1..603
FT /note="Carbon catabolite repressor protein 4 homolog 2"
FT /id="PRO_0000355045"
FT REGION 115..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 302
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:O95551"
SQ SEQUENCE 603 AA; 67053 MW; 23311F7627841B9E CRC64;
MLSVIRVHLP SEIPIVGCEL TPYVLVRRPD KNSTTDDVPE SAPLEGYFLR YRWYRVQSDK
KVTICSVHPT EQATLQCVFC SKRRSLVPKS YHCSPKCFTD AWQHHRTLHE RAAAENNANE
DDDLNRNNSA GSGSLAGSLS GSMSNLSIAN NGPAPFYPSN ITQKNGGETL VEVGGCKTYT
PTADDISHVL KFECVVANAE TKQIVGHPST ILTSRVIPAP SPSPRKLIPV NGADGMGHLD
QDARIQSAGS FTVLSYNILS DTSASSDLYS YCPPWALSWP YRRQNLLREI VGYRADVVCL
QEVQSDHFHE IFAPELDKHG YQALYKRKTN EVLSGSTSAI DGCATFFRRD RFSHVKKYDV
EFNKAAQSLT DALIPQAQKR TALNRLVKDN IALIVVLEAK FGNQPTDPSG KRQLICVANT
HVNVQQDLKD VKLWQVHTLL KGLEKIAASA DIPMLVCGDF NTLPGSAPHT LLVMGKVDPM
HPDLAVDPLN ILRPHTKLTH QLPLVSAYSS FVRKGIMGLG LEQHRRRIDL NTNEPLFTNC
TRDFIGTHDY IFYTADTLMV ESLLELLDED GLRKDTALPS PEWSSNHIAL LAEFRCTPRT
RRG
