CCR4_CANAL
ID CCR4_CANAL Reviewed; 787 AA.
AC Q5A761; A0A1D8PE96; O94047;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 {ECO:0000305};
DE EC=3.1.13.4;
DE AltName: Full=Carbon catabolite repressor protein 4;
DE AltName: Full=Cytoplasmic deadenylase;
DE AltName: Full=Glucose-repressible alcohol dehydrogenase transcriptional effector;
GN Name=CCR4; OrderedLocusNames=CAALFM_C108220WA;
GN ORFNames=Ca41C10.13c, CaO19.12567, CaO19.5101;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 199-787.
RC STRAIN=1161;
RA Taylor K., Harris D., Barrell B.G., Rajandream M.A.;
RT "Candida albicans strain 1161 genome pilot sequencing project.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as catalytic component of the CCR4-NOT core complex,
CC which in the nucleus seems to be a general transcription factor, and in
CC the cytoplasm the major mRNA deadenylase involved in mRNA turnover (By
CC similarity). Ccr4 has 3'-5' RNase activity with a strong preference for
CC polyadenylated substrates and also low exonuclease activity towards
CC single-stranded DNA (By similarity). {ECO:0000250|UniProtKB:P31384}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CCR4/nocturin family. {ECO:0000305}.
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DR EMBL; CP017623; AOW26462.1; -; Genomic_DNA.
DR EMBL; AL033501; CAA21997.1; -; Genomic_DNA.
DR PIR; T18239; T18239.
DR RefSeq; XP_717638.2; XM_712545.2.
DR AlphaFoldDB; Q5A761; -.
DR SMR; Q5A761; -.
DR STRING; 237561.Q5A761; -.
DR PRIDE; Q5A761; -.
DR GeneID; 3640749; -.
DR KEGG; cal:CAALFM_C108220WA; -.
DR CGD; CAL0000194050; CCR4.
DR VEuPathDB; FungiDB:C1_08220W_A; -.
DR eggNOG; KOG0620; Eukaryota.
DR HOGENOM; CLU_016428_4_1_1; -.
DR InParanoid; Q5A761; -.
DR OMA; YHFPSDH; -.
DR OrthoDB; 724242at2759; -.
DR PRO; PR:Q5A761; -.
DR Proteomes; UP000000559; Chromosome 1.
DR GO; GO:0030014; C:CCR4-NOT complex; IBA:GO_Central.
DR GO; GO:0030015; C:CCR4-NOT core complex; IEA:EnsemblFungi.
DR GO; GO:0016593; C:Cdc73/Paf1 complex; IEA:EnsemblFungi.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000932; C:P-body; IEA:EnsemblFungi.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004535; F:poly(A)-specific ribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000076; P:DNA replication checkpoint signaling; IEA:EnsemblFungi.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0048478; P:replication fork protection; IEA:EnsemblFungi.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR GO; GO:0007089; P:traversing start control point of mitotic cell cycle; IEA:EnsemblFungi.
DR Gene3D; 3.60.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF03372; Exo_endo_phos; 1.
DR Pfam; PF13855; LRR_8; 1.
DR SMART; SM00369; LRR_TYP; 3.
DR SUPFAM; SSF56219; SSF56219; 1.
DR PROSITE; PS51450; LRR; 3.
PE 3: Inferred from homology;
KW Cytoplasm; Exonuclease; Hydrolase; Leucine-rich repeat; Magnesium;
KW Metal-binding; Nuclease; Nucleus; Reference proteome; Repeat; RNA-binding;
KW Transcription; Transcription regulation.
FT CHAIN 1..787
FT /note="CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4"
FT /id="PRO_0000290607"
FT REPEAT 291..313
FT /note="LRR 1"
FT REPEAT 315..336
FT /note="LRR 2"
FT REPEAT 338..359
FT /note="LRR 3"
FT REPEAT 361..382
FT /note="LRR 4"
FT REPEAT 384..406
FT /note="LRR 5"
FT REGION 37..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 507
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:O95551"
FT CONFLICT 562
FT /note="I -> V (in Ref. 4; CAA21997)"
FT /evidence="ECO:0000305"
FT CONFLICT 748
FT /note="G -> R (in Ref. 4; CAA21997)"
FT /evidence="ECO:0000305"
FT CONFLICT 784
FT /note="N -> T (in Ref. 4; CAA21997)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 787 AA; 90325 MW; 44A05726795171FA CRC64;
MNITNKFQHT PIQGQPQSNL QAQQILLSQL HQGQAQQQQQ QQQQQQQQQQ SSAGVLGSGF
QPSDNFGESL NQNIYQNNYQ RAQPSLQQQF FPQFQQSQQQ PSQQANNASQ LQQPYQPAQQ
MVRQTSHFQQ QQQQQPSSQQ FYSQQQASVL QQQQQQQIQL QSAHQQQSSL LNMNSINVDN
PNSVYWQHQQ QLCQISRGSN VPHYYARQYA SNSRKAKNPY SEVKSVGLVE ATRSMVASLE
DEEEKKKKPI NYQGTPTTSA ALLHNKKSTQ DIFEDDSMEE QRMRLKTQGK QLWCQLDLSG
QGLVNISSKL FHYDFLESLY LSNNKLNSIP SSISKLRNLR TLDLSHNRIN ELPEELGLCF
NLRYLFLFDN NIKTLPYSFG NMIELLFIGI EGNPLEPSIA NLIAEKGTKE LIATLRDQTT
VKRTPKPRCW LTLEDDGEVV DSDEVYKVEP ESSDNFTVLS YNTLCQHYAT PKMYKFTPSW
ALQWDYRKNL LEKEVLNYNT DIVCMQEVET KTFQEFWLPV MTANGYKGYF FSKTRSKTMS
ETDSKKVDGC ATFFKNDKFS LIHKQNFEYN SVCMGSDKYK KTKDLFNRFM NKDNIALISY
LQHKESGEKI AVVNTHLHWD PAFNDVKALQ VGILLEELQG IIKKYRHTNS NEDIKNSSIV
VCGDFNSVKD SAVYQLFSTG ASKGHEDMNG RDYGKFTEDG FHHPFKLKSA YEAVGELPFT
NLTPAFTDNI DYIWYSTPTL QVKGLLGGVD EEYTSHSIGF PDANFPSDHV PILAKFQLKK
GKKNHSV
