CCR4_NEUCR
ID CCR4_NEUCR Reviewed; 793 AA.
AC Q9C2R2; V5ILT7;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 {ECO:0000305};
DE EC=3.1.13.4;
DE AltName: Full=Carbon catabolite repressor protein 4;
DE AltName: Full=Cytoplasmic deadenylase;
DE AltName: Full=Glucose-repressible alcohol dehydrogenase transcriptional effector;
GN Name=ccr4; ORFNames=NCU07779;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Acts as catalytic component of the CCR4-NOT core complex,
CC which in the nucleus seems to be a general transcription factor, and in
CC the cytoplasm the major mRNA deadenylase involved in mRNA turnover (By
CC similarity). Ccr4 has 3'-5' RNase activity with a strong preference for
CC polyadenylated substrates and also low exonuclease activity towards
CC single-stranded DNA (By similarity). {ECO:0000250|UniProtKB:P31384}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CCR4/nocturin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC28578.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL513410; CAC28578.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM002240; ESA42355.1; -; Genomic_DNA.
DR EMBL; CM002240; ESA42356.1; -; Genomic_DNA.
DR RefSeq; XP_011394724.1; XM_011396422.1.
DR RefSeq; XP_011394725.1; XM_011396423.1.
DR AlphaFoldDB; Q9C2R2; -.
DR SMR; Q9C2R2; -.
DR STRING; 5141.EFNCRP00000007975; -.
DR PRIDE; Q9C2R2; -.
DR EnsemblFungi; ESA42355; ESA42355; NCU07779.
DR EnsemblFungi; ESA42356; ESA42356; NCU07779.
DR GeneID; 3874847; -.
DR KEGG; ncr:NCU07779; -.
DR VEuPathDB; FungiDB:NCU07779; -.
DR HOGENOM; CLU_016428_4_0_1; -.
DR InParanoid; Q9C2R2; -.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0030014; C:CCR4-NOT complex; IBA:GO_Central.
DR GO; GO:0030015; C:CCR4-NOT core complex; IEA:EnsemblFungi.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000932; C:P-body; IEA:EnsemblFungi.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004535; F:poly(A)-specific ribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IBA:GO_Central.
DR Gene3D; 3.60.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF03372; Exo_endo_phos; 1.
DR SMART; SM00369; LRR_TYP; 2.
DR SUPFAM; SSF56219; SSF56219; 1.
DR PROSITE; PS51450; LRR; 3.
PE 3: Inferred from homology;
KW Cytoplasm; Exonuclease; Hydrolase; Leucine-rich repeat; Magnesium;
KW Metal-binding; Nuclease; Nucleus; Reference proteome; Repeat; RNA-binding;
KW Transcription; Transcription regulation.
FT CHAIN 1..793
FT /note="CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4"
FT /id="PRO_0000290615"
FT REPEAT 241..264
FT /note="LRR 1"
FT REPEAT 266..287
FT /note="LRR 2"
FT REPEAT 289..310
FT /note="LRR 3"
FT REPEAT 312..334
FT /note="LRR 4"
FT REPEAT 335..354
FT /note="LRR 5"
FT REGION 50..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 764..793
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 770..793
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 446
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:O95551"
SQ SEQUENCE 793 AA; 88752 MW; 78E3900D086A6D48 CRC64;
MADGHRYMQQ SFSTPLPTQF ASNNNLATGL LLNSLLNQFI ADPSAAQQQA AAQANPAHSS
ATQINRGMYG QNHPQGHNPR LNGAAPGRQP NMPMFYNHVP QQGHPHQGHN AHHQVLQAGH
SGHGARNDLM SHSTFSSGIM GNASPYTTNN LQNGHSVAAR GGPAEQPANE HWQKQMRLKE
ESDRAHSAMT EQHQPHYYAR LKAPENKGIG GSLTAGGANA SGDSEEEVRR RPYQVEKRNR
RQDWHNLDMS GQGLRALSSA LFSYDFLVEL YIASNRLTFL PAEIGKLRHL KILEASNNLL
SELPPEIGMC TSLEKLLLFD NQIRDLPYEL GSLYKLDILG IEGNPINPGL REEIVERGTK
SLINSLLEQA PVPLPPSPRK PIVVQEDVSP SLERIKVMTW NILCDKFATT NMYGYTPTGA
LSWEYRKERI LQEIRDRDVD MLCLQEIATD VFRDFFSPEL AQNDYKGVHW PRPKAKTMNE
KDAAAVDGCA IFYKGSKWIL LDKQLIDYAN IAINRPDMKN QHDIFNRVMP KDNIGIICFF
ESRRTGARVI VANTHLAWEP TLADVKLVQT AILMENITKY AEKYVRWQPL KDKRGIQIPQ
SVSVESDIPK PEMPEPGPSQ EYRSNTDIPL IVCGDYNSTQ ESSVYELLSM GRVTPEQSDF
GGHQYGNFTR DGVAHPFSMR SAYVHLNGTP DELSFTNYVP GFQEVIDYIW YSTNTLEVVE
LLGPPDQNHL KRVPGFPNYH FPADHIQIMA EFVIKQRKGE KVKVIHGSGG GASGQQQQQQ
QLEGGQDFGS GSK
