CCR4_NOSCE
ID CCR4_NOSCE Reviewed; 476 AA.
AC C4V7I7;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Probable CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 {ECO:0000305};
DE EC=3.1.13.4;
DE AltName: Full=Carbon catabolite repressor protein 4;
DE AltName: Full=Cytoplasmic deadenylase;
DE AltName: Full=Glucose-repressible alcohol dehydrogenase transcriptional effector;
GN Name=CCR4; ORFNames=NCER_100420;
OS Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX NCBI_TaxID=578460;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BRL01;
RX PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT pathogen of honey bees.";
RL PLoS Pathog. 5:E1000466-E1000466(2009).
CC -!- FUNCTION: Acts as catalytic component of the CCR4-NOT core complex,
CC which in the nucleus seems to be a general transcription factor, and in
CC the cytoplasm the major mRNA deadenylase involved in mRNA turnover (By
CC similarity). Ccr4 has 3'-5' RNase activity with a strong preference for
CC polyadenylated substrates and also low exonuclease activity towards
CC single-stranded DNA (By similarity). {ECO:0000250|UniProtKB:P31384}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Component of the CCR4-NOT core complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CCR4/nocturin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ACOL01000021; EEQ82814.1; -; Genomic_DNA.
DR RefSeq; XP_002996485.1; XM_002996439.1.
DR AlphaFoldDB; C4V7I7; -.
DR SMR; C4V7I7; -.
DR STRING; 578460.C4V7I7; -.
DR EnsemblFungi; EEQ82814; EEQ82814; NCER_100420.
DR KEGG; nce:NCER_100420; -.
DR VEuPathDB; MicrosporidiaDB:NCER_100420; -.
DR HOGENOM; CLU_016428_4_2_1; -.
DR InParanoid; C4V7I7; -.
DR OMA; DHFLMLT; -.
DR Proteomes; UP000009082; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004535; F:poly(A)-specific ribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR Gene3D; 3.60.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR025875; Leu-rich_rpt_4.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF03372; Exo_endo_phos; 1.
DR Pfam; PF12799; LRR_4; 1.
DR SMART; SM00369; LRR_TYP; 3.
DR SUPFAM; SSF56219; SSF56219; 1.
DR PROSITE; PS51450; LRR; 3.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; Exonuclease; Hydrolase; Leucine-rich repeat;
KW Magnesium; Metal-binding; Nuclease; Nucleus; Reference proteome; Repeat;
KW Repressor; RNA-binding; Transcription; Transcription regulation.
FT CHAIN 1..476
FT /note="Probable CCR4-Not complex 3'-5'-exoribonuclease
FT subunit Ccr4"
FT /id="PRO_0000388441"
FT REPEAT 22..43
FT /note="LRR 1"
FT REPEAT 45..66
FT /note="LRR 2"
FT REPEAT 68..89
FT /note="LRR 3"
FT REPEAT 91..113
FT /note="LRR 4"
FT REPEAT 114..135
FT /note="LRR 5"
FT BINDING 220
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:O95551"
SQ SEQUENCE 476 AA; 55490 MW; F366A86A6BF03CEB CRC64;
MEEIQNLKKK NYKSVQKNYS EDYLGLDFSY QGLKHLSYSL FQLTFIKELN LKGNDLENIP
GDIYILKNLE ILNLSKNKIK FLPAKIGKMI NLKELYLSDN FISNIPMELG SLYNCTVFEI
NNNPLISPFN LLYKDKKLLQ YCREHNTNYG PPADRTWLDT VIKKDMSEIT FSCGTYNILS
NYSAVRLGYP PTWVLNPDYR KENILHNICS INVDILCLQE VETYNYEDFY KDQLELRCEY
SSVFQPKGRS KNLTDSKSVD GCATFWKKSK FKIKENLVID FYSKFINDYR FNKNINLVSR
YGKKDNIALI SIFEISQTKQ TLIVVNVHLY WDPEYEDIKF VQAIILLEEL EKVSKCYKNP
SIVLLGDFNS LQNSSVYSFI TQNSVSNTNL CKYNIGFIPG HFLKLSDAYL SEENDFTNFT
PTFKGVIDFI FYSDTLELRS ILSTIENEYC DQVVGLPNIH FPSDHIFLAS KFKLKK
