CCR6_HUMAN
ID CCR6_HUMAN Reviewed; 374 AA.
AC P51684; E1P5C6; P78553; Q92846;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 203.
DE RecName: Full=C-C chemokine receptor type 6;
DE Short=C-C CKR-6;
DE Short=CC-CKR-6;
DE Short=CCR-6;
DE AltName: Full=Chemokine receptor-like 3;
DE Short=CKR-L3;
DE AltName: Full=DRY6;
DE AltName: Full=G-protein coupled receptor 29;
DE AltName: Full=GPR-CY4;
DE Short=GPRCY4;
DE AltName: Full=LARC receptor;
DE AltName: CD_antigen=CD196;
GN Name=CCR6; Synonyms=CKRL3, CMKBR6, GPR29, STRL22;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=9169459; DOI=10.1074/jbc.272.23.14893;
RA Baba M., Imai T., Nishimura M., Kakizaki M., Takagi S., Hieshima K.,
RA Nomiyama H., Yoshie O.;
RT "Identification of CCR6, the specific receptor for a novel lymphocyte-
RT directed CC chemokine LARC.";
RL J. Biol. Chem. 272:14893-14898(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RA Lautens L.L., Modi W., Bonner T.I.;
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=8886020; DOI=10.1006/bbrc.1996.1595;
RA Zaballos A., Varona R., Gutierrez J., Lind P., Marquez G.;
RT "Molecular cloning and RNA expression of two new human chemokine receptor-
RT like genes.";
RL Biochem. Biophys. Res. Commun. 227:846-853(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA McCoy R., Perlmutter D.H.;
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9070937; DOI=10.1006/geno.1996.4544;
RA Liao F., Lee H.-H., Farber J.M.;
RT "Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor
RT related to chemokine receptors and located on chromosome 6q27.";
RL Genomics 40:175-180(1997).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Warren C.N., Aronstam R.S., Sharma S.V.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP DISULFIDE BOND, MUTAGENESIS OF CYS-36; CYS-57; CYS-118; CYS-131; CYS-138;
RP CYS-168; CYS-197; CYS-233; CYS-266; CYS-288; CYS-309; CYS-310; CYS-336 AND
RP CYS-348, AND SUBCELLULAR LOCATION.
RX PubMed=12081481; DOI=10.1021/bi025855y;
RA Ai L.S., Liao F.;
RT "Mutating the four extracellular cysteines in the chemokine receptor CCR6
RT reveals their differing roles in receptor trafficking, ligand binding, and
RT signaling.";
RL Biochemistry 41:8332-8341(2002).
RN [11]
RP FUNCTION, AND BINDING TO CCL20; DEFB4 AND DEFB4A.
RX PubMed=20068036; DOI=10.1074/jbc.m109.091090;
RA Roehrl J., Yang D., Oppenheim J.J., Hehlgans T.;
RT "Specific binding and chemotactic activity of mBD4 and its functional
RT orthologue hBD2 to CCR6-expressing cells.";
RL J. Biol. Chem. 285:7028-7034(2010).
RN [12]
RP REVIEW, AND FUNCTION.
RX PubMed=21376174; DOI=10.1016/j.yexcr.2010.12.018;
RA Ito T., Carson W.F. IV, Cavassani K.A., Connett J.M., Kunkel S.L.;
RT "CCR6 as a mediator of immunity in the lung and gut.";
RL Exp. Cell Res. 317:613-619(2011).
RN [13]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23765988; DOI=10.1002/jcp.24418;
RA Caballero-Campo P., Buffone M.G., Benencia F., Conejo-Garcia J.R.,
RA Rinaudo P.F., Gerton G.L.;
RT "A role for the chemokine receptor CCR6 in mammalian sperm motility and
RT chemotaxis.";
RL J. Cell. Physiol. 229:68-78(2014).
RN [14]
RP FUNCTION, TISSUE SPECIFICITY, AND BINDING TO DEFB1.
RX PubMed=25122636; DOI=10.1126/scitranslmed.3009071;
RA Diao R., Fok K.L., Chen H., Yu M.K., Duan Y., Chung C.M., Li Z., Wu H.,
RA Li Z., Zhang H., Ji Z., Zhen W., Ng C.F., Gui Y., Cai Z., Chan H.C.;
RT "Deficient human beta-defensin 1 underlies male infertility associated with
RT poor sperm motility and genital tract infection.";
RL Sci. Transl. Med. 6:249RA108-249RA108(2014).
RN [15]
RP REVIEW, AND FUNCTION.
RX PubMed=25585877; DOI=10.1016/j.cyto.2014.11.029;
RA Lee A.Y., Phan T.K., Hulett M.D., Koerner H.;
RT "The relationship between CCR6 and its binding partners: does the CCR6-
RT CCL20 axis have to be extended?";
RL Cytokine 72:97-101(2015).
CC -!- FUNCTION: Receptor for the C-C type chemokine CCL20 (PubMed:9169459).
CC Binds to CCL20 and subsequently transduces a signal by increasing the
CC intracellular calcium ion levels (PubMed:20068036). Although CCL20 is
CC its major ligand it can also act as a receptor for non-chemokine
CC ligands such as beta-defensins (PubMed:25585877). Binds to defensin
CC DEFB1 leading to increase in intracellular calcium ions and cAMP
CC levels. Its binding to DEFB1 is essential for the function of DEFB1 in
CC regulating sperm motility and bactericidal activity (PubMed:25122636).
CC Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic
CC effects (PubMed:20068036). The ligand-receptor pair CCL20-CCR6 is
CC responsible for the chemotaxis of dendritic cells (DC), effector/
CC memory T-cells and B-cells and plays an important role at skin and
CC mucosal surfaces under homeostatic and inflammatory conditions, as well
CC as in pathology, including cancer and various autoimmune diseases.
CC CCR6-mediated signals are essential for immune responses to microbes in
CC the intestinal mucosa and in the modulation of inflammatory responses
CC initiated by tissue insult and trauma (PubMed:21376174). CCR6 is
CC essential for the recruitment of both the pro-inflammatory IL17
CC producing helper T-cells (Th17) and the regulatory T-cells (Treg) to
CC sites of inflammation. Required for the normal migration of Th17 cells
CC in Peyers-patches and other related tissue sites of the intestine and
CC plays a role in regulating effector T-cell balance and distribution in
CC inflamed intestine. Plays an important role in the coordination of
CC early thymocyte precursor migration events important for normal
CC subsequent thymocyte precursor development, but is not required for the
CC formation of normal thymic natural regulatory T-cells (nTregs).
CC Required for optimal differentiation of DN2 and DN3 thymocyte
CC precursors. Essential for B-cell localization in the subepithelial dome
CC of Peyers-patches and for efficient B-cell isotype switching to IgA in
CC the Peyers-patches. Essential for appropriate anatomical distribution
CC of memory B-cells in the spleen and for the secondary recall response
CC of memory B-cells (By similarity). Positively regulates sperm motility
CC and chemotaxis via its binding to CCL20 (PubMed:23765988).
CC {ECO:0000250|UniProtKB:O54689, ECO:0000269|PubMed:20068036,
CC ECO:0000269|PubMed:23765988, ECO:0000269|PubMed:25122636,
CC ECO:0000269|PubMed:9169459, ECO:0000303|PubMed:21376174,
CC ECO:0000303|PubMed:25585877}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23765988};
CC Multi-pass membrane protein {ECO:0000255}. Cell surface
CC {ECO:0000269|PubMed:12081481}.
CC -!- TISSUE SPECIFICITY: Sperm. Mainly localized in the tail and in the
CC postacrosomal region but is also found in the midpiece and basal region
CC in a small percentage of sperm cells. Reduced levels found in the
CC sperms of asthenozoospermia and leukocytospermia patients (at protein
CC level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in
CC lymphocytes, T-cells and B-cells but not in natural killer cells,
CC monocytes or granulocytes. {ECO:0000269|PubMed:23765988,
CC ECO:0000269|PubMed:25122636, ECO:0000269|PubMed:8886020}.
CC -!- INDUCTION: By IL2/interleukin-2.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-6 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB02144.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Wikipedia; Note=CC chemokine receptors entry;
CC URL="https://en.wikipedia.org/wiki/CC_chemokine_receptors";
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DR EMBL; U45984; AAB62714.1; -; Genomic_DNA.
DR EMBL; Z79784; CAB02144.1; ALT_INIT; Genomic_DNA.
DR EMBL; U60000; AAB06949.1; -; mRNA.
DR EMBL; U68030; AAC51124.1; -; mRNA.
DR EMBL; U68032; AAC51125.1; -; Genomic_DNA.
DR EMBL; AY242126; AAO92293.1; -; mRNA.
DR EMBL; AL121935; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW47506.1; -; Genomic_DNA.
DR EMBL; CH471051; EAW47507.1; -; Genomic_DNA.
DR EMBL; BC037960; AAH37960.1; -; mRNA.
DR CCDS; CCDS5298.1; -.
DR PIR; JC5068; JC5068.
DR RefSeq; NP_004358.2; NM_004367.5.
DR RefSeq; NP_113597.2; NM_031409.3.
DR PDB; 6WWZ; EM; 3.34 A; R=2-374.
DR PDBsum; 6WWZ; -.
DR AlphaFoldDB; P51684; -.
DR SMR; P51684; -.
DR BioGRID; 107640; 88.
DR DIP; DIP-5865N; -.
DR IntAct; P51684; 1.
DR MINT; P51684; -.
DR STRING; 9606.ENSP00000343952; -.
DR BindingDB; P51684; -.
DR ChEMBL; CHEMBL4423; -.
DR GuidetoPHARMACOLOGY; 63; -.
DR GlyGen; P51684; 2 sites.
DR iPTMnet; P51684; -.
DR PhosphoSitePlus; P51684; -.
DR BioMuta; CCR6; -.
DR DMDM; 2851567; -.
DR MassIVE; P51684; -.
DR PaxDb; P51684; -.
DR PeptideAtlas; P51684; -.
DR PRIDE; P51684; -.
DR ProteomicsDB; 56368; -.
DR Antibodypedia; 2930; 822 antibodies from 39 providers.
DR DNASU; 1235; -.
DR Ensembl; ENST00000341935.10; ENSP00000343952.5; ENSG00000112486.18.
DR Ensembl; ENST00000349984.6; ENSP00000339393.4; ENSG00000112486.18.
DR Ensembl; ENST00000400926.5; ENSP00000383715.2; ENSG00000112486.18.
DR Ensembl; ENST00000643861.1; ENSP00000493637.1; ENSG00000112486.18.
DR GeneID; 1235; -.
DR KEGG; hsa:1235; -.
DR MANE-Select; ENST00000341935.10; ENSP00000343952.5; NM_031409.4; NP_113597.2.
DR UCSC; uc003qvm.5; human.
DR CTD; 1235; -.
DR DisGeNET; 1235; -.
DR GeneCards; CCR6; -.
DR HGNC; HGNC:1607; CCR6.
DR HPA; ENSG00000112486; Tissue enriched (lymphoid).
DR MalaCards; CCR6; -.
DR MIM; 601835; gene.
DR neXtProt; NX_P51684; -.
DR OpenTargets; ENSG00000112486; -.
DR Orphanet; 220393; Diffuse cutaneous systemic sclerosis.
DR Orphanet; 220402; Limited cutaneous systemic sclerosis.
DR PharmGKB; PA26171; -.
DR VEuPathDB; HostDB:ENSG00000112486; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234667; -.
DR HOGENOM; CLU_009579_8_3_1; -.
DR InParanoid; P51684; -.
DR OMA; KEICDHR; -.
DR OrthoDB; 981680at2759; -.
DR PhylomeDB; P51684; -.
DR TreeFam; TF330966; -.
DR PathwayCommons; P51684; -.
DR Reactome; R-HSA-1461957; Beta defensins.
DR Reactome; R-HSA-380108; Chemokine receptors bind chemokines.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; P51684; -.
DR BioGRID-ORCS; 1235; 9 hits in 1068 CRISPR screens.
DR ChiTaRS; CCR6; human.
DR GeneWiki; C-C_chemokine_receptor_type_6; -.
DR GenomeRNAi; 1235; -.
DR Pharos; P51684; Tchem.
DR PRO; PR:P51684; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; P51684; protein.
DR Bgee; ENSG00000112486; Expressed in lymph node and 77 other tissues.
DR Genevisible; P51684; HS.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0036126; C:sperm flagellum; IDA:UniProtKB.
DR GO; GO:0097225; C:sperm midpiece; IDA:UniProtKB.
DR GO; GO:0097524; C:sperm plasma membrane; IDA:UniProtKB.
DR GO; GO:0097228; C:sperm principal piece; IDA:UniProtKB.
DR GO; GO:0019957; F:C-C chemokine binding; IDA:UniProtKB.
DR GO; GO:0016493; F:C-C chemokine receptor activity; IDA:UniProtKB.
DR GO; GO:0004950; F:chemokine receptor activity; TAS:ProtInc.
DR GO; GO:0038023; F:signaling receptor activity; TAS:ProtInc.
DR GO; GO:0019722; P:calcium-mediated signaling; IMP:UniProtKB.
DR GO; GO:0060326; P:cell chemotaxis; IDA:UniProtKB.
DR GO; GO:0006968; P:cellular defense response; TAS:ProtInc.
DR GO; GO:0006935; P:chemotaxis; IDA:UniProtKB.
DR GO; GO:0002407; P:dendritic cell chemotaxis; TAS:BHF-UCL.
DR GO; GO:1904155; P:DN2 thymocyte differentiation; ISS:UniProtKB.
DR GO; GO:1904156; P:DN3 thymocyte differentiation; ISS:UniProtKB.
DR GO; GO:0006959; P:humoral immune response; TAS:ProtInc.
DR GO; GO:0006955; P:immune response; IBA:GO_Central.
DR GO; GO:0048290; P:isotype switching to IgA isotypes; ISS:UniProtKB.
DR GO; GO:0002523; P:leukocyte migration involved in inflammatory response; ISS:UniProtKB.
DR GO; GO:0072676; P:lymphocyte migration; ISS:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:2000510; P:positive regulation of dendritic cell chemotaxis; IBA:GO_Central.
DR GO; GO:0060474; P:positive regulation of flagellated sperm motility involved in capacitation; IDA:UniProtKB.
DR GO; GO:2000404; P:regulation of T cell migration; ISS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR GO; GO:0072678; P:T cell migration; ISS:UniProtKB.
DR GO; GO:0072679; P:thymocyte migration; ISS:UniProtKB.
DR InterPro; IPR004067; Chemokine_CCR6.
DR InterPro; IPR000355; Chemokine_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR PANTHER; PTHR10489:SF611; PTHR10489:SF611; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00657; CCCHEMOKINER.
DR PRINTS; PR01529; CHEMOKINER6.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Membrane; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..374
FT /note="C-C chemokine receptor type 6"
FT /id="PRO_0000069286"
FT TOPO_DOM 1..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..74
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..119
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..141
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..159
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..238
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..279
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..303
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..321
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..374
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 118..197
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 36
FT /note="C->A: No loss of calcium flux but loss of chemotaxis
FT in response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 36
FT /note="C->D,G: Loss of calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 36
FT /note="C->S: No loss of calcium flux but loss of chemotaxis
FT in response to CCL20 stimulation, impaired CCL20-binding
FT and no effect on cell surface expression; when associated
FT with or without S-288. Loss of calcium flux in response to
FT CCL20 stimulation and significant reduction in cell surface
FT expression; when associated with S-118; S-197 and S-288."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 57
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 118
FT /note="C->S: Loss of calcium flux and chemotaxis in
FT response to CCL20 stimulation, impaired CCL20-binding and
FT significant reduction in cell surface expression; when
FT associated with or without S-197. Loss of calcium flux in
FT response to CCL20 stimulation and significant reduction in
FT cell surface expression; when associated with S-36; S-197
FT and S-288."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 131
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 138
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 168
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 197
FT /note="C->S: Loss of calcium flux and chemotaxis in
FT response to CCL20 stimulation, impaired CCL20-binding and
FT significant reduction in cell surface expression; when
FT associated with or without S-118. Loss of calcium flux in
FT response to CCL20 stimulation and significant reduction in
FT cell surface expression; when associated with S-36; S-118
FT and S-288."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 233
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 266
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 288
FT /note="C->A,G,R: No loss of calcium flux but loss of
FT chemotaxis in response to CCL20 stimulation. No effect on
FT cell surface expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 288
FT /note="C->S: No loss of calcium flux but loss of chemotaxis
FT in response to CCL20 stimulation, impaired CCL20-binding
FT and no effect on cell surface expression; when associated
FT with or without S-36. Loss of calcium flux in response to
FT CCL20 stimulation and significant reduction in cell surface
FT expression; when associated with S-36; S-118 and S-197."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 309
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 310
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on cell surface
FT expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 336
FT /note="C->S: Reduced calcium flux and chemotaxis in
FT response to CCL20 stimulation, and reduced CCL20-binding.
FT No effect on cell surface expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT MUTAGEN 348
FT /note="C->S: No effect on calcium flux and chemotaxis in
FT response to CCL20 stimulation. No effect on CCL20-binding
FT and cell surface expression."
FT /evidence="ECO:0000269|PubMed:12081481"
FT CONFLICT 60
FT /note="G -> A (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 74
FT /note="Y -> N (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="L -> V (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="S -> T (in Ref. 5; AAC51124/AAC51125)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="T -> S (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="Q -> L (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="E -> V (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="I -> F (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT CONFLICT 370..374
FT /note="SSFTM -> VVLHYVIES (in Ref. 4; AAB06949)"
FT /evidence="ECO:0000305"
FT STRAND 31..33
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 40..72
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 81..97
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 100..108
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 115..148
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 150..156
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 161..185
FT /evidence="ECO:0007829|PDB:6WWZ"
FT STRAND 186..189
FT /evidence="ECO:0007829|PDB:6WWZ"
FT STRAND 191..194
FT /evidence="ECO:0007829|PDB:6WWZ"
FT STRAND 196..199
FT /evidence="ECO:0007829|PDB:6WWZ"
FT STRAND 203..205
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 207..241
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 249..279
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 288..319
FT /evidence="ECO:0007829|PDB:6WWZ"
FT HELIX 321..334
FT /evidence="ECO:0007829|PDB:6WWZ"
SQ SEQUENCE 374 AA; 42494 MW; D7F963534E990BC4 CRC64;
MSGESMNFSD VFDSSEDYFV SVNTSYYSVD SEMLLCSLQE VRQFSRLFVP IAYSLICVFG
LLGNILVVIT FAFYKKARSM TDVYLLNMAI ADILFVLTLP FWAVSHATGA WVFSNATCKL
LKGIYAINFN CGMLLLTCIS MDRYIAIVQA TKSFRLRSRT LPRSKIICLV VWGLSVIISS
STFVFNQKYN TQGSDVCEPK YQTVSEPIRW KLLMLGLELL FGFFIPLMFM IFCYTFIVKT
LVQAQNSKRH KAIRVIIAVV LVFLACQIPH NMVLLVTAAN LGKMNRSCQS EKLIGYTKTV
TEVLAFLHCC LNPVLYAFIG QKFRNYFLKI LKDLWCVRRK YKSSGFSCAG RYSENISRQT
SETADNDNAS SFTM
