CCRM_BRUME
ID CCRM_BRUME Reviewed; 403 AA.
AC Q8YFS6;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=DNA methyltransferase CcrM {ECO:0000250|UniProtKB:Q2YMK2};
DE Short=M.CcrM;
DE EC=2.1.1.72;
DE AltName: Full=Adenine-specific methyltransferase BabI;
DE AltName: Full=Probable type II methyltransferase M.BmeLORF1444P {ECO:0000303|PubMed:12654995};
DE Short=M.BmeLORF1444P {ECO:0000303|PubMed:12654995};
GN Name=ccrM; OrderedLocusNames=BMEI1444;
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA Haselkorn R., Kyrpides N.C., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
RN [2]
RP NOMENCLATURE, AND SUBTYPE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: A beta subtype methylase that recognizes the double-stranded
CC sequence 5'-GANTC-3' and methylates A-2 on both strands
CC (PubMed:12654995) (By similarity). CcrM-mediated methylation has
CC important cellular functions. Contributes to the accurate cell-cycle
CC control of DNA replication and cellular morphology (By similarity).
CC {ECO:0000250|UniProtKB:O30569, ECO:0000250|UniProtKB:Q2YMK2,
CC ECO:0000303|PubMed:12654995}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AE008917; AAL52625.1; -; Genomic_DNA.
DR PIR; AF3432; AF3432.
DR AlphaFoldDB; Q8YFS6; -.
DR SMR; Q8YFS6; -.
DR STRING; 224914.BMEI1444; -.
DR REBASE; 5746; M.BmeLORF1444P.
DR PRIDE; Q8YFS6; -.
DR EnsemblBacteria; AAL52625; AAL52625; BMEI1444.
DR KEGG; bme:BMEI1444; -.
DR eggNOG; COG2189; Bacteria.
DR OMA; WRKANPM; -.
DR Proteomes; UP000000419; Chromosome I.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR002941; DNA_methylase_N4/N6.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR040843; RAMA.
DR InterPro; IPR001091; RM_Methyltransferase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF01555; N6_N4_Mtase; 1.
DR Pfam; PF18755; RAMA; 1.
DR PRINTS; PR00508; S21N4MTFRASE.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; Methyltransferase; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..403
FT /note="DNA methyltransferase CcrM"
FT /id="PRO_0000363193"
FT DOMAIN 297..399
FT /note="RAMA"
FT /evidence="ECO:0000255"
SQ SEQUENCE 403 AA; 45065 MW; 3ABB8BE232CB72F9 CRC64;
MALTAYLPYA VTIGTSFLRS QVIEYPMSLV RLAHELPIEA PRTAWLDSII KGDCVSALER
LSDHSVDVIF ADPPYNLQLG GDLHRPDQSM VSAVDDHWDQ FESFQAYDAF TRAWLLACRR
VLKPNGTIWV IGSYHNIFRV GTQLQDLGFW LLNDIVWRKT NPMPNFRGRR FQNAHETLIW
ASRDQKGKGY TFNYEAMKAA NDDVQMRSDW LFPICTGSER LKDENGDKVH PTQKPEALLA
RIMMASSKPG DVILDPFFGS GTTGAVAKRL GRHFVGIERE QPYIDAATAR INAVEPLGKA
ELTVMTGKRA EPRVAFTSVM EAGLLRPGTV LCDERRRFAA IVRADGTLTA NGEAGSIHRI
GARVQGFDAC NGWTFWHFEE NGVLKPIDAL RKIIREQMAA AGA
