CCS1_CHLRE
ID CCS1_CHLRE Reviewed; 613 AA.
AC Q8GTZ9; A8JK64; O50041; Q8GTZ8; Q8H0J3;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Cytochrome c biogenesis protein CCS1, chloroplastic;
DE AltName: Full=C-type cytochrome synthesis protein 1;
DE Flags: Precursor;
GN Name=CCS1; ORFNames=CHLREDRAFT_111044, CHLREDRAFT_195343;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=21gr / CC-1690;
RX PubMed=9395519; DOI=10.1074/jbc.272.50.31747;
RA Inoue K., Dreyfuss B.W., Kindle K.L., Stern D.B., Merchant S.,
RA Sodeinde O.A.;
RT "Ccs1, a nuclear gene required for the post-translational assembly of
RT chloroplast c-type cytochromes.";
RL J. Biol. Chem. 272:31747-31754(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, POSSIBLE
RP TOPOLOGY, VARIANT CCS1-3 GLY-536 DEL, VARIANT CCS1-4 GLN-144 DEL, MUTANT
RP CCS1-AC206 VAL-251 INS, MUTANT GLY-260, AND MUTAGENESIS OF CYS-199; HIS-274
RP AND ASP-348.
RC STRAIN=137c / CC-125, ccs1-2, ccs1-3, ccs1-4, and ccs1-ac206;
RX PubMed=12427747; DOI=10.1074/jbc.m208652200;
RA Dreyfuss B.W., Hamel P.P., Nakamoto S.S., Merchant S.;
RT "Functional analysis of a divergent system II protein, Ccs1, involved in c-
RT type cytochrome biogenesis.";
RL J. Biol. Chem. 278:2604-2613(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-143 AND 189-586.
RC STRAIN=CC-503;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
RN [4]
RP GENETIC ANALYSIS, AND SUGGESTION OF SUBUNIT.
RX PubMed=9504916; DOI=10.1093/genetics/148.2.681;
RA Xie Z., Culler D., Dreyfuss B.W., Kuras R., Wollman F.-A.,
RA Girard-Bascou J., Merchant S.;
RT "Genetic analysis of chloroplast c-type cytochrome assembly in
RT Chlamydomonas reinhardtii: one chloroplast locus and at least four nuclear
RT loci are required for heme attachment.";
RL Genetics 148:681-692(1998).
RN [5]
RP SUBUNIT.
RC STRAIN=137c / CC-125;
RX PubMed=12427766; DOI=10.1074/jbc.m208651200;
RA Hamel P.P., Dreyfuss B.W., Xie Z., Gabilly S.T., Merchant S.;
RT "Essential histidine and tryptophan residues in CcsA, a system II polytopic
RT cytochrome c biogenesis protein.";
RL J. Biol. Chem. 278:2593-2603(2003).
CC -!- FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome
CC c6 and cytochrome f) at the step of heme attachment.
CC {ECO:0000269|PubMed:12427747}.
CC -!- SUBUNIT: May interact with ccsA. {ECO:0000269|PubMed:12427766}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305|PubMed:12427747}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:12427747}.
CC -!- MISCELLANEOUS: Algae lacking CCS1 are unable to grow phototrophically;
CC they specifically accumulate apocytochrome c6 and apocytochrome f.
CC -!- MISCELLANEOUS: A mutant with a small stroma insert (ccs1-ac206) does
CC not produce holocytochromes.
CC -!- SIMILARITY: Belongs to the Ccs1/CcsB family. {ECO:0000305}.
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DR EMBL; U70999; AAB95195.1; -; mRNA.
DR EMBL; U71000; AAB95196.1; -; Genomic_DNA.
DR EMBL; AY095302; AAM47499.1; -; Genomic_DNA.
DR EMBL; AY095301; AAM47499.1; JOINED; Genomic_DNA.
DR EMBL; AY095298; AAM23259.1; -; Genomic_DNA.
DR EMBL; AY095304; AAM44130.1; -; Genomic_DNA.
DR EMBL; AY095303; AAM44130.1; JOINED; Genomic_DNA.
DR EMBL; AY095299; AAM23262.1; -; Genomic_DNA.
DR EMBL; DS497507; EDO95667.1; -; Genomic_DNA.
DR EMBL; DS497231; EDO95734.1; -; Genomic_DNA.
DR PIR; T09105; T09105.
DR RefSeq; XP_001694401.1; XM_001694349.1.
DR AlphaFoldDB; Q8GTZ9; -.
DR STRING; 3055.EDO95734; -.
DR PaxDb; Q8GTZ9; -.
DR EnsemblPlants; PNW73842; PNW73842; CHLRE_13g575000v5.
DR Gramene; PNW73842; PNW73842; CHLRE_13g575000v5.
DR eggNOG; ENOG502QRFF; Eukaryota.
DR HOGENOM; CLU_034630_0_0_1; -.
DR InParanoid; Q8GTZ9; -.
DR OMA; RFWIDYT; -.
DR OrthoDB; 509749at2759; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-KW.
DR HAMAP; MF_01392; CytC_Ccs1; 1.
DR InterPro; IPR023494; Cyt_c_bgen_Ccs1/CcsB/ResB.
DR InterPro; IPR007816; ResB-like_domain.
DR PANTHER; PTHR31566; PTHR31566; 1.
DR Pfam; PF05140; ResB; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Cytochrome c-type biogenesis; Membrane; Plastid; Thylakoid;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..36
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 37..92
FT /note="Thylakoid"
FT /evidence="ECO:0000255"
FT CHAIN 93..613
FT /note="Cytochrome c biogenesis protein CCS1, chloroplastic"
FT /id="PRO_0000363638"
FT TOPO_DOM 93..121
FT /note="Stromal"
FT /evidence="ECO:0000305"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 143..178
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 200..268
FT /note="Stromal"
FT /evidence="ECO:0000305"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 290..613
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT REGION 63..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 409..429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 144..613
FT /note="Missing (in ccs1-4; abrogates production of c-type
FT holocytochrome)"
FT VARIANT 536..613
FT /note="Missing (in ccs1-3; abrogates production of c-type
FT holocytochrome)"
FT MUTAGEN 199
FT /note="C->A: No effect on c-type holocytochrome formation."
FT /evidence="ECO:0000269|PubMed:12427747"
FT MUTAGEN 251
FT /note="V->RESCACQRTAAALLLRFHSVAADPSPLLSFVLVAQV: In ccs1-
FT ac206; abrogates production of c-type holocytochrome."
FT MUTAGEN 260
FT /note="G->N: In ccs1-2; about 2% protein accumulates,
FT allows about 5% holocytochrome f but no holocytochrome c6
FT formation."
FT MUTAGEN 274
FT /note="H->A: Abrogates production of c-type holocytochrome;
FT about 5% CCS1 protein accumulates."
FT /evidence="ECO:0000269|PubMed:12427747"
FT MUTAGEN 348
FT /note="D->A: No effect on c-type holocytochrome formation."
FT /evidence="ECO:0000269|PubMed:12427747"
SQ SEQUENCE 613 AA; 64897 MW; 8BC93B67DC9DA1A3 CRC64;
MQPYASVSGR CLSRPDALHV IPFGRPLQAI AGRRFVRCFA KGGQPGDKKK LNVTDKLRLG
NTPPTLDVLK APRPTDAPSA IDDAPSTSGL GLGGGVASPR TLVQSNAVQV AWRRLMKELS
SLPRAIAIMA LIAVLSGLGT FIPQNKSIEY YLVNYPDGAE KVLGFLTGDL ILTLQLDHIY
TADYFYLSMG LLAASLAACT YTRQWPAVKV AQRWRFLTQP KSLLKQGRTE VLPNARVSDL
GAILLQRGYQ VFVKDGSLYG FKGLAGKLGP IGVHAALLLC LFGTAWSGFG TLKGNVMCPE
GQDFQVASFL QPSSPIASMP ASASNVIHVN KFTIDYRPDG SVAQFYSDLS LLDPAQGGKE
MMRKTISVND PFRFNGVTMY QTDWSLSAVT LRVLGQDAPL ARAAQAAEAQ AAASTSGPTS
SASSTSDALP QQRTAFNLPM ASLEGKPGVA GRLWATFLPL AEPGQDGSAP KGISILARDP
QSVVFYDAKG QFVGVRRPGS GKPIEVEGLA LVVEDVTGAT GLELKSDPGV PAVYAGFGGL
MVTTLISYLS HSQVWALQQG SSLFVSGRTN RAKLAFDREL DDILNAVPEL PPTAATTVAS
SASTAAPAPT AKQ
