CCS1_SYNY3
ID CCS1_SYNY3 Reviewed; 458 AA.
AC P73912;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Cytochrome c biogenesis protein CcsB;
GN Name=ccsB; Synonyms=ccs1; OrderedLocusNames=slr2087;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 1-24.
RX PubMed=10542282; DOI=10.1074/jbc.274.45.32396;
RA Tichy M., Vermaas W.;
RT "Accumulation of pre-apocytochrome f in a Synechocystis sp. PCC 6803 mutant
RT impaired in cytochrome c maturation.";
RL J. Biol. Chem. 274:32396-32401(1999).
RN [3]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=12427747; DOI=10.1074/jbc.m208652200;
RA Dreyfuss B.W., Hamel P.P., Nakamoto S.S., Merchant S.;
RT "Functional analysis of a divergent system II protein, Ccs1, involved in c-
RT type cytochrome biogenesis.";
RL J. Biol. Chem. 278:2604-2613(2003).
CC -!- FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome
CC c6 and cytochrome f) at the step of heme attachment. {ECO:0000250}.
CC -!- FUNCTION: Probably not involved in heme transport or reduction as
CC mutants deleted for the first 24 residues cannot be rescued by growth
CC in the presence of heme or reductant. {ECO:0000269|PubMed:10542282}.
CC -!- SUBUNIT: May interact with CcsA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:12427747}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12427747}.
CC -!- DISRUPTION PHENOTYPE: Bacteria lacking the N-terminus of CcsB are able
CC to grow anaerobically but not aerobically, suggesting its function is
CC essential for aerobic growth. In these bacteria reduced amounts of some
CC holo-c-type cytochromes are observed (cytochrome f and cytochrome
CC c550); unprocessed precursor apocytochrome f accumulates but is not
CC thought to be integrated into the cytochrome b6-f complex. Holo-
CC cytochrome c553 (also known as cytochrome c6) does not accumulate
CC however. {ECO:0000269|PubMed:10542282}.
CC -!- SIMILARITY: Belongs to the Ccs1/CcsB family. {ECO:0000305}.
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DR EMBL; BA000022; BAA17976.1; -; Genomic_DNA.
DR PIR; S75114; S75114.
DR AlphaFoldDB; P73912; -.
DR IntAct; P73912; 10.
DR STRING; 1148.1653059; -.
DR PaxDb; P73912; -.
DR EnsemblBacteria; BAA17976; BAA17976; BAA17976.
DR KEGG; syn:slr2087; -.
DR eggNOG; COG1333; Bacteria.
DR InParanoid; P73912; -.
DR OMA; RFWIDYT; -.
DR PhylomeDB; P73912; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01392; CytC_Ccs1; 1.
DR InterPro; IPR023494; Cyt_c_bgen_Ccs1/CcsB/ResB.
DR InterPro; IPR007816; ResB-like_domain.
DR PANTHER; PTHR31566; PTHR31566; 1.
DR Pfam; PF05140; ResB; 2.
PE 1: Evidence at protein level;
KW Cytochrome c-type biogenesis; Membrane; Reference proteome; Thylakoid;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..458
FT /note="Cytochrome c biogenesis protein CcsB"
FT /id="PRO_0000363635"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..84
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000305"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..458
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000305"
FT MUTAGEN 1..24
FT /note="Missing: Unable to grow under aerobic conditions;
FT under anaerobic conditions significantly reduced amounts of
FT holo-c-type cytochromes accumulate."
FT /evidence="ECO:0000269|PubMed:10542282"
SQ SEQUENCE 458 AA; 50621 MW; FA0FB46D6535A7EF CRC64;
MTIANPSPSN FFQQLGRQCL KTLADLRLAI ALLLLIAVFS ISGTVIEQGE SLSFYQQNYP
EDPALFGFLS WQVILQLGLN QVYRTWWFLG LLILFGSSLT ACTFNRQFPA LKAARSWQFY
HQPRQFKKLA LSFSLPDGDI NKIESLLRDR GYKIFQEGDS VYARKGLMGK VGPIIVHGAM
LIILGGAIWG ALTGFFAQHM IPSGETFQVS NIIEKGPLAD SQIPKDWGIK VNRFWINYTE
NGAIDQFYSD LSVVNNQGEE LDRQTISVNH PLRHRGVTFY QTNWGIAGVK VQLNNSPVLQ
LPMAPLQTAN GGQLWGAYIP TKTDFSEGAA LLVKDLQGTM IIYDQEGNLT DAVRAGSTVE
INGVNITIKE LVGSTGLQIK ADPGIPFVYL GFGLLMVGVM MSYVSHSQVW LLSVDGDGQR
EIYLGGRTNR AQVAFEREIL AIAEEAEVSS KTEAKVNA
