CCSA_CHLRE
ID CCSA_CHLRE Reviewed; 353 AA.
AC P48269; B7U1H0;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Cytochrome c biogenesis protein CcsA;
GN Name=ccsA;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CC-400;
RX PubMed=7610180; DOI=10.1104/pp.108.2.843;
RA Chen Z.Y., Moroney J.V.;
RT "Identification of a Chlamydomonas reinhardtii chloroplast gene with
RT significant homology to bacterial genes involved in cytochrome c
RT biosynthesis.";
RL Plant Physiol. 108:843-844(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=CC-503;
RX PubMed=8617725; DOI=10.1074/jbc.271.9.4632;
RA Xie Z., Merchant S.;
RT "The plastid-encoded ccsA gene is required for heme attachment to
RT chloroplast c-type cytochromes.";
RL J. Biol. Chem. 271:4632-4639(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [4]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
RN [5]
RP START SITE IDENTIFICATION, TOPOLOGY, AND MUTAGENESIS OF MET-1; MET-20;
RP HIS-212; TRP-279; TRP-288; TRP-290; ASP-291; TRP-296; TRP-301; HIS-309 AND
RP HIS-347.
RC STRAIN=137c / CC-125;
RX PubMed=12427766; DOI=10.1074/jbc.m208651200;
RA Hamel P.P., Dreyfuss B.W., Xie Z., Gabilly S.T., Merchant S.;
RT "Essential histidine and tryptophan residues in CcsA, a system II polytopic
RT cytochrome c biogenesis protein.";
RL J. Biol. Chem. 278:2593-2603(2003).
RN [6]
RP REVIEW.
RX PubMed=9693743; DOI=10.1016/s0005-2728(98)00085-1;
RA Xie Z., Merchant S.;
RT "A novel pathway for cytochromes c biogenesis in chloroplasts.";
RL Biochim. Biophys. Acta 1365:309-318(1998).
CC -!- FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome
CC c6 and cytochrome f) at the step of heme attachment.
CC {ECO:0000269|PubMed:8617725}.
CC -!- SUBUNIT: May interact with Ccs1. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Met-1 is the initiator; however when Met-1 is mutated a
CC low amount of protein may be produced, probably from Met-20.
CC -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U15556; AAA76600.1; -; Genomic_DNA.
DR EMBL; U09190; AAB03815.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50117.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00929.1; -; Genomic_DNA.
DR PIR; T07998; T07998.
DR PIR; T08001; T08001.
DR RefSeq; NP_958384.1; NC_005353.1.
DR AlphaFoldDB; P48269; -.
DR SMR; P48269; -.
DR STRING; 3055.DAA00929; -.
DR TCDB; 9.B.14.3.3; the putative heme handling protein (hhp) family.
DR PaxDb; P48269; -.
DR PRIDE; P48269; -.
DR GeneID; 2717027; -.
DR KEGG; cre:ChreCp028; -.
DR eggNOG; ENOG502QU0T; Eukaryota.
DR HOGENOM; CLU_049710_2_4_1; -.
DR InParanoid; P48269; -.
DR OrthoDB; 1361264at2759; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01391; CytC_CcsA; 1.
DR InterPro; IPR002541; Cyt_c_assembly.
DR InterPro; IPR017562; Cyt_c_biogenesis_CcsA.
DR InterPro; IPR045062; Cyt_c_biogenesis_CcsA/CcmC.
DR PANTHER; PTHR30071; PTHR30071; 1.
DR Pfam; PF01578; Cytochrom_C_asm; 1.
DR TIGRFAMs; TIGR03144; cytochr_II_ccsB; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Cytochrome c-type biogenesis; Membrane; Plastid;
KW Reference proteome; Thylakoid; Transmembrane; Transmembrane helix.
FT CHAIN 1..353
FT /note="Cytochrome c biogenesis protein CcsA"
FT /id="PRO_0000201600"
FT TRANSMEM 15..32
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 33..208
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:12427766"
FT TRANSMEM 209..233
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 234..260
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:12427766"
FT TRANSMEM 261..278
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 279..295
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:12427766"
FT TRANSMEM 296..313
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 314..323
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:12427766"
FT TRANSMEM 324..342
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 343..353
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:12427766"
FT MUTAGEN 1
FT /note="M->D: Almost no c-type holocytochrome formation."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 20
FT /note="M->A: No effect on c-type holocytochrome formation."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 212
FT /note="H->D: Abrogates production of c-type
FT holocytochrome."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 279
FT /note="W->A: Abrogates production of c-type
FT holocytochrome."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 284
FT /note="W->A: Severely reduces amounts of c-type
FT holocytochrome produced."
FT MUTAGEN 288..290
FT /note="WSW->ASA: Reduced amounts of c-type holocytochromes
FT produced; a more severe reduction than seen with either
FT single mutation."
FT MUTAGEN 288
FT /note="W->A: Reduced amounts of c-type holocytochromes
FT produced."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 290
FT /note="W->A: Reduced amounts of c-type holocytochromes
FT produced."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 291
FT /note="D->A: Abrogates production of c-type holocytochrome.
FT No assembly of cytochrome c6 occurs."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 291
FT /note="D->E,N: Abrogates production of c-type
FT holocytochrome. Permits small amounts of cytochrome c6
FT assembly."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 296
FT /note="W->A: No effect on activity."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 301
FT /note="W->A: No effect on activity."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 309
FT /note="H->E: Abrogates production of c-type
FT holocytochrome."
FT /evidence="ECO:0000269|PubMed:12427766"
FT MUTAGEN 347
FT /note="H->A,E: Abrogates production of c-type
FT holocytochrome."
FT /evidence="ECO:0000269|PubMed:12427766"
FT CONFLICT 342
FT /note="I -> T (in Ref. 1; AAA76600)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 353 AA; 39983 MW; 3D46ABDF51044C4B CRC64;
MNFVNLEQIE NSLRNATFCM LFLTTFLYWF YTAFYSTNPQ QIINPLSLTN IKTNFTYPDG
FQANNINVST TYLPINPVLN TEREEQPEAN GTNGLLGVSS LVVNLKSIAI PRIMMGVSNL
LLVLLLLVRW EKSGHFPLSN LYESLMFLAW CCTFLYLLYC TSFTLLVEKM LGSLIAPCSL
LMNAFATFSL PKEMQQASPL VPALQSNWLM MHVTVMIISY ATLIIGSLLS ILFLILFKHK
KGTPKKYDNF INNLDALSYR IIGLGFPFLT IGILSGAVWA NEAWGSYWSW DPKETWALLT
WLVFAIYLHT RLTKGWEGEK PAIIAAVGFL VVWFCYLGVN LIGEGLHSYG FFN
