ID   CCSA_CUCSA              Reviewed;         323 AA.
AC   Q2QD41; Q4VZL9;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Cytochrome c biogenesis protein CcsA {ECO:0000255|HAMAP-Rule:MF_01391};
GN   Name=ccsA {ECO:0000255|HAMAP-Rule:MF_01391};
OS   Cucumis sativus (Cucumber).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Baekmibaekdadagi;
RX   PubMed=16362300; DOI=10.1007/s00299-005-0097-y;
RA   Kim J.-S., Jung J.D., Lee J.-A., Park H.-W., Oh K.-H., Jeong W.J.,
RA   Choi D.-W., Liu J.R., Cho K.Y.;
RT   "Complete sequence and organization of the cucumber (Cucumis sativus L. cv.
RT   Baekmibaekdadagi) chloroplast genome.";
RL   Plant Cell Rep. 25:334-340(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Borszczagowski;
RX   PubMed=17607527; DOI=10.2478/s11658-007-0029-7;
RA   Plader W.W., Yukawa Y., Sugiura M., Malepszy S.;
RT   "The complete structure of the cucumber (Cucumis sativus L.) chloroplast
RT   genome: its composition and comparative analysis.";
RL   Cell. Mol. Biol. Lett. 12:584-594(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Chipper, and cv. Gy14;
RX   PubMed=17546086; DOI=10.1139/g07-003;
RA   Chung S.-M., Gordon V.S., Staub J.E.;
RT   "Sequencing cucumber (Cucumis sativus L.) chloroplast genomes identifies
RT   differences between chilling-tolerant and -susceptible cucumber lines.";
RL   Genome 50:215-225(2007).
CC   -!- FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome
CC       c6 and cytochrome f) at the step of heme attachment.
CC       {ECO:0000255|HAMAP-Rule:MF_01391}.
CC   -!- SUBUNIT: May interact with Ccs1. {ECO:0000255|HAMAP-Rule:MF_01391}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000255|HAMAP-Rule:MF_01391}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01391}.
CC   -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC       {ECO:0000255|HAMAP-Rule:MF_01391}.
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DR   EMBL; DQ119058; AAZ94698.1; -; Genomic_DNA.
DR   EMBL; AJ970307; CAJ00808.1; -; Genomic_DNA.
DR   EMBL; DQ865975; ABI97462.1; -; Genomic_DNA.
DR   EMBL; DQ865976; ABI98793.1; -; Genomic_DNA.
DR   RefSeq; YP_247649.1; NC_007144.1.
DR   AlphaFoldDB; Q2QD41; -.
DR   SMR; Q2QD41; -.
DR   GeneID; 3429362; -.
DR   KEGG; csv:3429362; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01391; CytC_CcsA; 1.
DR   InterPro; IPR002541; Cyt_c_assembly.
DR   InterPro; IPR017562; Cyt_c_biogenesis_CcsA.
DR   InterPro; IPR045062; Cyt_c_biogenesis_CcsA/CcmC.
DR   PANTHER; PTHR30071; PTHR30071; 1.
DR   Pfam; PF01578; Cytochrom_C_asm; 1.
DR   TIGRFAMs; TIGR03144; cytochr_II_ccsB; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Cytochrome c-type biogenesis; Membrane; Plastid; Thylakoid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..323
FT                   /note="Cytochrome c biogenesis protein CcsA"
FT                   /id="PRO_0000353744"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        261..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   VARIANT         93..106
FT                   /note="KYKNHLSVSAITAP -> NIKTIKCKRNNRA (in cv.
FT                   Borszczagowski)"
FT   VARIANT         125
FT                   /note="Q -> P (in cv. Borszczagowski)"
FT   VARIANT         192
FT                   /note="Y -> S (in cv. Borszczagowski)"
FT   VARIANT         225
FT                   /note="Y -> C (in cv. Borszczagowski)"
FT   VARIANT         317
FT                   /note="S -> P (in cv. Borszczagowski)"
SQ   SEQUENCE   323 AA;  36962 MW;  5D5F2CEFDE771849 CRC64;
     MIFSTFEHIL THISFSVISI VITIQLITLL INETVGLYVS SEKGMIATFF CITGLLVTRW
     IYLRHLPLSD LYESLLFLSW AFSIIHLFTY FKKYKNHLSV SAITAPSTIF TQGFATSGFL
     TEMHQSPLLV PALQSHWLMM HVSMMVLGYA ALLCGSLFSV ALIVITFQKA IRIFCKNNNL
     LNALFSFNEI QYINEGNYFV RNTSFFSSKN YYKSQLIQQL DHWSYRIISL GFIFLSIGIL
     SGAVWANETW GSYWNWDPKE TWAFITWTIF AIYLHTRTNK NFEGVNSAIV ASMGFLIIWI
     CYFGVNLLGI GLHSYGSFTL TSN