ID   CCSA_LOTJA              Reviewed;         323 AA.
AC   Q9BBP4;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Cytochrome c biogenesis protein CcsA {ECO:0000255|HAMAP-Rule:MF_01391};
GN   Name=ccsA {ECO:0000255|HAMAP-Rule:MF_01391};
OS   Lotus japonicus (Lotus corniculatus var. japonicus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Miyakojima MG-20;
RX   PubMed=11214967; DOI=10.1093/dnares/7.6.323;
RA   Kato T., Kaneko T., Sato S., Nakamura Y., Tabata S.;
RT   "Complete structure of the chloroplast genome of a legume, Lotus
RT   japonicus.";
RL   DNA Res. 7:323-330(2000).
CC   -!- FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome
CC       c6 and cytochrome f) at the step of heme attachment.
CC       {ECO:0000255|HAMAP-Rule:MF_01391}.
CC   -!- SUBUNIT: May interact with Ccs1. {ECO:0000255|HAMAP-Rule:MF_01391}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000255|HAMAP-Rule:MF_01391}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01391}.
CC   -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC       {ECO:0000255|HAMAP-Rule:MF_01391}.
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DR   EMBL; AP002983; BAB33244.1; -; Genomic_DNA.
DR   RefSeq; NP_084844.1; NC_002694.1.
DR   AlphaFoldDB; Q9BBP4; -.
DR   SMR; Q9BBP4; -.
DR   GeneID; 802903; -.
DR   OMA; WLVMHVS; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01391; CytC_CcsA; 1.
DR   InterPro; IPR002541; Cyt_c_assembly.
DR   InterPro; IPR017562; Cyt_c_biogenesis_CcsA.
DR   InterPro; IPR045062; Cyt_c_biogenesis_CcsA/CcmC.
DR   PANTHER; PTHR30071; PTHR30071; 1.
DR   Pfam; PF01578; Cytochrom_C_asm; 1.
DR   TIGRFAMs; TIGR03144; cytochr_II_ccsB; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Cytochrome c-type biogenesis; Membrane; Plastid; Thylakoid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..323
FT                   /note="Cytochrome c biogenesis protein CcsA"
FT                   /id="PRO_0000201605"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        229..249
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        262..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
SQ   SEQUENCE   323 AA;  37186 MW;  5A034E3E2829FE35 CRC64;
     MIFSTLEHIL THISFSVVSI VISIHLITLF VNQIVGFYDS SKKGMIITFL CITGLLITRW
     FFSGHLPFSD LYESLIFLSW GFSIFYMVPR FKKQKNDLST IIAPSVIFTQ GFATSGLLTE
     MHQSVILVPA LQSHWLMMHV SMMILGYAAL LCGSLLSVAI LVITFQELIP ILGKSKRLSF
     LYESFDYAEI KYINMNERNN VLRKTSFSSY RNYYRYQFIQ QLDRWGYRII SLGFIFLTIG
     ILSGAVWANE AWGSYWNWDP KETWAFITWT IFAIYLHSRK NKKLEGLNSS IVASIGFLII
     WICYFGVNLL GIGLHNYGSF TSN