CCSA_SYNY3
ID CCSA_SYNY3 Reviewed; 334 AA.
AC P72978; Q55316; Q79F46;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Cytochrome c biogenesis protein CcsA {ECO:0000255|HAMAP-Rule:MF_01391};
GN Name=ccsA {ECO:0000255|HAMAP-Rule:MF_01391}; OrderedLocusNames=sll1513;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Lee K., Fukuzawa H., Ohyama K.;
RT "A photosensitive cyanobacterial mutant generated by insertional
RT mutagenesis of orf334 homologous to liverwort chloroplast orf320.";
RL Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND ATTEMPTED DISRUPTION
RP PHENOTYPE.
RX PubMed=9187367; DOI=10.1016/s0014-5793(97)00421-3;
RA Huebschmann T., Wilde A., Elanskaya I., Shestakov S.V., Boerner T.;
RT "A putative cytochrome c biogenesis gene in Synechocystis sp. PCC 6803.";
RL FEBS Lett. 408:201-205(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [4]
RP ATTEMPTED DISRUPTION PHENOTYPE.
RX PubMed=10542282; DOI=10.1074/jbc.274.45.32396;
RA Tichy M., Vermaas W.;
RT "Accumulation of pre-apocytochrome f in a Synechocystis sp. PCC 6803 mutant
RT impaired in cytochrome c maturation.";
RL J. Biol. Chem. 274:32396-32401(1999).
CC -!- FUNCTION: Required during biogenesis of c-type cytochromes (cytochrome
CC c6 and cytochrome f) at the step of heme attachment.
CC {ECO:0000255|HAMAP-Rule:MF_01391}.
CC -!- SUBUNIT: May interact with ccs1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane {ECO:0000255|HAMAP-
CC Rule:MF_01391}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01391}.
CC -!- INDUCTION: By light. {ECO:0000269|PubMed:9187367}.
CC -!- DISRUPTION PHENOTYPE: Cannot be insertionally inactivated suggesting it
CC has an essential function (PubMed:9187367, PubMed:10542282).
CC {ECO:0000269|PubMed:10542282, ECO:0000269|PubMed:9187367}.
CC -!- SIMILARITY: Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.
CC {ECO:0000255|HAMAP-Rule:MF_01391}.
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DR EMBL; D38599; BAA22777.1; -; Genomic_DNA.
DR EMBL; Z72480; CAA96562.1; -; Genomic_DNA.
DR EMBL; BA000022; BAA16997.1; -; Genomic_DNA.
DR PIR; S74957; S74957.
DR AlphaFoldDB; P72978; -.
DR SMR; P72978; -.
DR IntAct; P72978; 5.
DR STRING; 1148.1652072; -.
DR PaxDb; P72978; -.
DR EnsemblBacteria; BAA16997; BAA16997; BAA16997.
DR KEGG; syn:sll1513; -.
DR eggNOG; COG0755; Bacteria.
DR InParanoid; P72978; -.
DR OMA; WLVMHVS; -.
DR PhylomeDB; P72978; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0017004; P:cytochrome complex assembly; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01391; CytC_CcsA; 1.
DR InterPro; IPR002541; Cyt_c_assembly.
DR InterPro; IPR017562; Cyt_c_biogenesis_CcsA.
DR InterPro; IPR045062; Cyt_c_biogenesis_CcsA/CcmC.
DR PANTHER; PTHR30071; PTHR30071; 1.
DR Pfam; PF01578; Cytochrom_C_asm; 1.
DR TIGRFAMs; TIGR03144; cytochr_II_ccsB; 1.
PE 2: Evidence at transcript level;
KW Cytochrome c-type biogenesis; Membrane; Reference proteome; Thylakoid;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..334
FT /note="Cytochrome c biogenesis protein CcsA"
FT /id="PRO_0000353723"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01391"
FT CONFLICT 173
FT /note="S -> C (in Ref. 2; CAA96562)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 334 AA; 36077 MW; F835B70699B80D95 CRC64;
MNLVSLESFL DNTAFLVLLL TMFAYWVAVV FPKPWLVQGA SGAMAIANLT ITALLGARWL
EAGYFPISNL YESLFFLAWG ITAVHFIAER MSQSRFVGAV TSPIALGIVA FAALTLPVDM
QQSAPLVPAL KSNWLMMHVS VMMVSYATLM VGSLLAIAFL FVTRGQAVEL RGSSVGTGGF
RQGLVKGNNL NPVGNLNPAL EGVSGNSGNV AVLEKTTSTP AITLSPQRLT LADTLDNISY
RIIGLGFPLL TIGIIAGAVW ANEAWGSYWS WDPKETWALI TWLVFAAYLH ARITKGWQGR
KPAILAASGF TVVWICYLGV NLLGKGLHSY GWFL
