CCSE2_MOUSE
ID CCSE2_MOUSE Reviewed; 833 AA.
AC Q3UHI0; B2RT11; Q2NKY3; Q3TA20; Q6ZPX3; Q9DAD5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Serine-rich coiled-coil domain-containing protein 2;
DE AltName: Full=Coiled-coil serine-rich protein 2;
DE AltName: Full=Granule cell antiserum positive protein 14;
DE AltName: Full=Protein GCAP14;
GN Name=Ccser2; Synonyms=Fam190b, Gcap14, Kiaa1128;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 538-833 (ISOFORM 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Spleen, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 454-833 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 57-728 (ISOFORMS 1/2).
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451, PHOSPHORYLATION [LARGE
RP SCALE ANALYSIS] AT SER-843 (ISOFORM 2), AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22673506; DOI=10.1016/j.febslet.2012.04.008;
RA Hosono H., Yamaguchi N., Oshima K., Matsuda T., Nadano D.;
RT "The murine Gcap14 gene encodes a novel microtubule binding and bundling
RT protein.";
RL FEBS Lett. 586:1426-1430(2012).
CC -!- FUNCTION: Microtubule-binding protein which might play a role in
CC microtubule bundling. {ECO:0000269|PubMed:22673506}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:22673506}. Note=Associates with microtubules in
CC interphase. Has diffuse expression throughout the cell during mitosis.
CC {ECO:0000269|PubMed:22673506}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3UHI0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UHI0-2; Sequence=VSP_029183;
CC -!- TISSUE SPECIFICITY: Expressed in brain (at protein level).
CC {ECO:0000269|PubMed:22673506}.
CC -!- SIMILARITY: Belongs to the CCSER family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24323.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK005931; BAB24323.1; ALT_INIT; mRNA.
DR EMBL; AK147383; BAE27877.1; -; mRNA.
DR EMBL; AK172153; BAE42850.1; -; mRNA.
DR EMBL; BC111465; AAI11466.1; -; mRNA.
DR EMBL; BC139090; AAI39091.1; -; mRNA.
DR EMBL; AK129295; BAC98105.1; -; mRNA.
DR CCDS; CCDS26948.1; -. [Q3UHI0-1]
DR CCDS; CCDS84113.1; -. [Q3UHI0-2]
DR RefSeq; NP_001334431.1; NM_001347502.1. [Q3UHI0-2]
DR RefSeq; NP_001334432.1; NM_001347503.1.
DR RefSeq; NP_081321.1; NM_027045.1. [Q3UHI0-1]
DR RefSeq; NP_082683.2; NM_028407.3.
DR AlphaFoldDB; Q3UHI0; -.
DR SMR; Q3UHI0; -.
DR BioGRID; 215684; 9.
DR IntAct; Q3UHI0; 9.
DR STRING; 10090.ENSMUSP00000087478; -.
DR iPTMnet; Q3UHI0; -.
DR PhosphoSitePlus; Q3UHI0; -.
DR EPD; Q3UHI0; -.
DR jPOST; Q3UHI0; -.
DR MaxQB; Q3UHI0; -.
DR PaxDb; Q3UHI0; -.
DR PRIDE; Q3UHI0; -.
DR ProteomicsDB; 279956; -. [Q3UHI0-1]
DR ProteomicsDB; 279957; -. [Q3UHI0-2]
DR Antibodypedia; 48922; 34 antibodies from 9 providers.
DR DNASU; 72972; -.
DR Ensembl; ENSMUST00000067700; ENSMUSP00000068550; ENSMUSG00000058690. [Q3UHI0-2]
DR Ensembl; ENSMUST00000090024; ENSMUSP00000087478; ENSMUSG00000058690. [Q3UHI0-1]
DR GeneID; 72972; -.
DR KEGG; mmu:72972; -.
DR UCSC; uc007tbn.1; mouse. [Q3UHI0-2]
DR UCSC; uc007tbo.1; mouse. [Q3UHI0-1]
DR CTD; 54462; -.
DR MGI; MGI:101859; Ccser2.
DR VEuPathDB; HostDB:ENSMUSG00000058690; -.
DR eggNOG; ENOG502QV4S; Eukaryota.
DR GeneTree; ENSGT00940000153912; -.
DR HOGENOM; CLU_010809_0_0_1; -.
DR InParanoid; Q3UHI0; -.
DR OMA; NCHQNSK; -.
DR OrthoDB; 1263802at2759; -.
DR PhylomeDB; Q3UHI0; -.
DR TreeFam; TF331021; -.
DR BioGRID-ORCS; 72972; 3 hits in 69 CRISPR screens.
DR ChiTaRS; Ccser2; mouse.
DR PRO; PR:Q3UHI0; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q3UHI0; protein.
DR Bgee; ENSMUSG00000058690; Expressed in rostral migratory stream and 246 other tissues.
DR ExpressionAtlas; Q3UHI0; baseline and differential.
DR Genevisible; Q3UHI0; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0015630; C:microtubule cytoskeleton; IDA:MGI.
DR GO; GO:0008017; F:microtubule binding; IDA:MGI.
DR GO; GO:0001578; P:microtubule bundle formation; IDA:MGI.
DR InterPro; IPR029627; CCSER.
DR InterPro; IPR029629; CCSER2.
DR PANTHER; PTHR22461; PTHR22461; 1.
DR PANTHER; PTHR22461:SF2; PTHR22461:SF2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..833
FT /note="Serine-rich coiled-coil domain-containing protein 2"
FT /id="PRO_0000309462"
FT REGION 178..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 478..508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 602..631
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 789..833
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 711..747
FT /evidence="ECO:0000255"
FT COMPBIAS 604..621
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 797..833
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 222
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7U1"
FT MOD_RES 451
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 775..833
FT /note="GGYCAPSFSPWQGSFQGMPRTVPPHRRQTSSTTAFQQPSQIYRPRPGKTNKA
FT TTYRGPQ -> PQVLQPSSSLPRSTDHAQGKLIKPQRTEAHSDYTVQGVCPGGAHPDGS
FT CTHGLQQDNSRGLQERPSSSSPQLTVDVVKYIPSETDLSMTLDAQEPHHLAEKKPSDLQ
FT FVTPPPQTPSQSSTVDQTKRGGRNQCPQPKSLQLLKPSNLSSLTPPPDSDSSPSRTSTC
FT KKAPGITPCHSKHQPTSNQNNPANHLNLKTSKLRPPSGSFKQKQISNPQVEPQNFQAKT
FT SIPRPLARPKELHAPHSGLHSGDCVASNRYSRLPKPKIH (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029183"
FT CONFLICT 61
FT /note="S -> P (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 94
FT /note="E -> D (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="H -> Y (in Ref. 1; BAE42850)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="T -> A (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="S -> P (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="I -> M (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 307
FT /note="A -> N (in Ref. 1; BAE42850 and 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 353
FT /note="V -> I (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="N -> S (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 424
FT /note="N -> K (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="M -> V (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 449
FT /note="F -> L (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 460
FT /note="S -> G (in Ref. 3; BAC98105)"
FT /evidence="ECO:0000305"
FT CONFLICT 479
FT /note="H -> Q (in Ref. 1; BAE42850)"
FT /evidence="ECO:0000305"
FT MOD_RES Q3UHI0-2:843
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 833 AA; 92932 MW; 18C871932C925CA8 CRC64;
MEEKTQIKTF LGSKLPKYGM KSVRSTLQPM PNGATVTLLG TSKSSNVKSY IKNNGSDCSL
SHSFNWRKTN KYQLGSQNTA ELNNIQSTHD KLIEPEQHAP APGTLDGHGI KGGLKSASLF
TSKLARPSTM FVSSAEELSQ KSFSGPSNLG KFTKGTLLGR TSYSSVNAKS HLNAFYGNRS
SGNVQKPRVN SCASRSSSGE SLAQSPDNAK SITCEKMVRS QSFSHSIQNV FLPPSSITRS
HSFNRAVDLT KPYQNQQLPV RVPLRSGMLT RSSLQSEVLN GNEHVGFGFN RPYAAAGKKL
ALSNGPAVSS TLVYRMAHPP LLKSSRPPFS GPMTVDSNKN PPADMCVEEE GMVSAQDSSP
GKDQELIENE SYRRDNDQTG KNESKVRYLS DDVDDISLSS LSSSDKNDLS EDFSDDFIDL
EDSNRTRITP EEMTLKEEKH ESRPSKDIFD SPKESEQAFS KAEEWIDISV SDRSECTKHT
SGNNLISPDT DYRAGSSFEL SPSDSSDGTY MWDEEGLEPI GSVHPVGSYE SSEMNSIDIL
NNLESCDLED DDLMLDVDLP EDAPLENVEC DNMNRFDRTD RNVRQSQDGF WKRPPQRWSG
QDHYHLSHPG HYHHHGQSDL SRGSPYRESP LGHFESYGGT PFFQAQKMFV DVPDNTVILD
EMTLRHMVQD CTAVKTQLLK LKRLLHQHDG SGSLHDVQLS LPSSPEPEDG DQIYKNEDLL
NEITQLKEEI KKKDEKIQLL EQQLATRCNC QQKSKEEKCT YADKYTQTPW RRIPGGYCAP
SFSPWQGSFQ GMPRTVPPHR RQTSSTTAFQ QPSQIYRPRP GKTNKATTYR GPQ
