ID   CCS_CITSI               Reviewed;         503 AA.
AC   Q9SEA0;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Capsanthin/capsorubin synthase, chromoplastic;
DE            EC=5.3.99.8;
DE   Flags: Precursor;
GN   Name=CCS;
OS   Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX   NCBI_TaxID=2711;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12549109;
RA   Xu C.J., Chen D.M., Zhang S.L.;
RT   "Molecular cloning of full length capsanthin/capsorubin synthase homologous
RT   gene from orange (Citrus sinensis).";
RL   Shi Yan Sheng Wu Xue Bao 34:147-150(2001).
CC   -!- FUNCTION: Catalyzes the conversion of the ubiquitous 5,6-
CC       epoxycarotenoids, antheraxanthin and violaxanthin, into capsanthin and
CC       capsorubin, respectively.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-violaxanthin = all-trans-capsorubin;
CC         Xref=Rhea:RHEA:21752, ChEBI:CHEBI:3378, ChEBI:CHEBI:35288;
CC         EC=5.3.99.8;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-antheraxanthin = all-trans-capsanthin;
CC         Xref=Rhea:RHEA:17373, ChEBI:CHEBI:3375, ChEBI:CHEBI:27867;
CC         EC=5.3.99.8;
CC   -!- PATHWAY: Carotenoid biosynthesis; capsanthin biosynthesis; capsanthin
CC       from antheraxanthin: step 1/1.
CC   -!- PATHWAY: Carotenoid biosynthesis; capsorubin biosynthesis; capsorubin
CC       from violaxanthin: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Plastid, chromoplast {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; AF169241; AAF18389.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9SEA0; -.
DR   SMR; Q9SEA0; -.
DR   STRING; 2711.XP_006487912.1; -.
DR   eggNOG; ENOG502QQD7; Eukaryota.
DR   UniPathway; UPA00806; UER00774.
DR   UniPathway; UPA00807; UER00775.
DR   GO; GO:0009509; C:chromoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0052727; F:capsanthin synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052728; F:capsorubin synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Chromoplast; Isomerase; NAD; Oxidoreductase;
KW   Plastid; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chromoplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..503
FT                   /note="Capsanthin/capsorubin synthase, chromoplastic"
FT                   /id="PRO_0000018437"
FT   BINDING         88..117
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   503 AA;  56663 MW;  2C957F0AA91075E6 CRC64;
     MATLLSPFSP SPLAKVSQII DSTSSPSFSL FPLGRQNACS RKADHHHHHR IRTSKFGNFL
     ELTPESVPEF LDFDLPWFHP SDRIRYDVII IGTGPAGLRL AEQVSSRHSV KVCCVDPSPL
     STWPNNYGVW VDEFEDIGLV DCLDKTWPMT CVFINDHKTK YLDRPYGRVS RNILKTKLLE
     NCVSNGVKFH KAKVWHVNHQ EFESSIVCDD GNEIKASLIV DASGFASSFV EYDKPRNHGY
     QIAHGILAEV ESHPFDLDKM VLMDWRDSHL GNEPYLRASN LKLPTFLYAM PFDSNLVFLE
     ETSLVSRPVL SYKEVKSRMA ARLRHMGIRV KRVIEDEKCL IPMGGPLPVI PQSVMAIGGT
     SGLIHPATGY MVARTMALAP ALADAIAECL GSTRMIRGRP LHQKVWNGLW PIDRRCNREF
     YSFGMETLLK LDLKGTRRFF DAFFDLNPYY WHGFLSSRLS LAELAGLSLS LFGHASNSSR
     LDIVTKCPVP LVKMMGNLAL ETI