CCW12_YEAST
ID CCW12_YEAST Reviewed; 133 AA.
AC Q12127; D6VYB0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Covalently-linked cell wall protein 12;
DE AltName: Full=Protein Alpha0.6;
DE Flags: Precursor;
GN Name=CCW12; OrderedLocusNames=YLR110C; ORFNames=L9354.7;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 90840 / EAY235 / FY23;
RX PubMed=9090053;
RX DOI=10.1002/(sici)1097-0061(19970315)13:3<241::aid-yea61>3.0.co;2-#;
RA Verhasselt P., Volckaert G.;
RT "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of
RT Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-
RT Arg3 and 23 new open reading frames, among which several homologies to
RT proteins involved in cell division control and to mammalian growth factors
RT and other animal proteins are found.";
RL Yeast 13:241-250(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9234669;
RX DOI=10.1002/(sici)1097-0061(199707)13:9<809::aid-yea141>3.0.co;2-1;
RA Seidel J., Tanner W.;
RT "Characterization of two new genes down-regulated by alpha-factor.";
RL Yeast 13:809-817(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP PROTEIN SEQUENCE OF 19-51, CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RX PubMed=10322008; DOI=10.1128/jb.181.10.3076-3086.1999;
RA Mrsa V., Ecker M., Strahl-Bolsinger S., Nimtz M., Lehle L., Tanner W.;
RT "Deletion of new covalently linked cell wall glycoproteins alters the
RT electrophoretic mobility of phosphorylated wall components of Saccharomyces
RT cerevisiae.";
RL J. Bacteriol. 181:3076-3086(1999).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=9613572; DOI=10.1007/s004380050706;
RA Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.;
RT "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall
RT proteins in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 258:53-59(1998).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP SUBCELLULAR LOCATION, AND GLYCOSYLATION.
RX PubMed=15165243; DOI=10.1111/j.1365-2958.2004.04064.x;
RA Hagen I., Ecker M., Lagorce A., Francois J.M., Sestak S., Rachel R.,
RA Grossmann G., Hauser N.C., Hoheisel J.D., Tanner W., Strahl S.;
RT "Sed1p and Srl1p are required to compensate for cell wall instability in
RT Saccharomyces cerevisiae mutants defective in multiple GPI-anchored
RT mannoproteins.";
RL Mol. Microbiol. 52:1413-1425(2004).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, AND GLYCOSYLATION AT ASN-21; ASN-81 AND
RP ASN-97.
RX PubMed=17315267; DOI=10.1002/yea.1465;
RA Ragni E., Sipiczki M., Strahl S.;
RT "Characterization of Ccw12p, a major key player in cell wall stability of
RT Saccharomyces cerevisiae.";
RL Yeast 24:309-319(2007).
RN [11]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-80, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- FUNCTION: Component of the cell wall. May play a role in the formation
CC of a tightly packed outer mannan layer, which protects the inner
CC glucan. {ECO:0000269|PubMed:17315267}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-
CC CWP).
CC -!- PTM: Extensively O-glycosylated; glycans consist probably of single
CC mannose residues. N-glycosylated. {ECO:0000269|PubMed:15165243,
CC ECO:0000269|PubMed:17315267}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- MISCELLANEOUS: Present with 158000 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: To yeast protein YDR134C. {ECO:0000305}.
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DR EMBL; Z73282; CAA97677.1; -; Genomic_DNA.
DR EMBL; U53878; AAB67555.1; -; Genomic_DNA.
DR EMBL; AY557929; AAS56255.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09426.1; -; Genomic_DNA.
DR PIR; S64947; S64947.
DR RefSeq; NP_013211.1; NM_001181997.1.
DR AlphaFoldDB; Q12127; -.
DR BioGRID; 31383; 305.
DR DIP; DIP-4570N; -.
DR IntAct; Q12127; 3.
DR MINT; Q12127; -.
DR STRING; 4932.YLR110C; -.
DR iPTMnet; Q12127; -.
DR MaxQB; Q12127; -.
DR PaxDb; Q12127; -.
DR PRIDE; Q12127; -.
DR EnsemblFungi; YLR110C_mRNA; YLR110C; YLR110C.
DR GeneID; 850800; -.
DR KEGG; sce:YLR110C; -.
DR SGD; S000004100; CCW12.
DR VEuPathDB; FungiDB:YLR110C; -.
DR eggNOG; ENOG502S42T; Eukaryota.
DR HOGENOM; CLU_105987_1_0_1; -.
DR InParanoid; Q12127; -.
DR OMA; SAHANVT; -.
DR BioCyc; YEAST:G3O-32257-MON; -.
DR PRO; PR:Q12127; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12127; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005934; C:cellular bud tip; IDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0043332; C:mating projection tip; IDA:SGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IMP:CACAO.
DR GO; GO:0000752; P:agglutination involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0000747; P:conjugation with cellular fusion; IMP:SGD.
DR InterPro; IPR025928; Flocculin_t3_rpt.
DR Pfam; PF13928; Flocculin_t3; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation; Direct protein sequencing;
KW Glycoprotein; GPI-anchor; Isopeptide bond; Lipoprotein; Membrane;
KW Reference proteome; Repeat; Secreted; Signal; Ubl conjugation.
FT SIGNAL 1..18
FT /evidence="ECO:0000269|PubMed:10322008"
FT CHAIN 19..112
FT /note="Covalently-linked cell wall protein 12"
FT /id="PRO_0000020879"
FT PROPEP 113..133
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000020880"
FT REPEAT 75..88
FT /note="1"
FT REPEAT 91..103
FT /note="2"
FT REGION 79..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 112
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 21
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17315267"
FT CARBOHYD 23
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 24
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 26
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 28
FT /note="O-linked (Man) serine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 31
FT /note="O-linked (Man) serine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 32
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 33
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 36
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 38
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 39
FT /note="O-linked (Man) serine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 46
FT /note="O-linked (Man) serine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 48
FT /note="O-linked (Man) threonine"
FT /evidence="ECO:0000269|PubMed:15165243"
FT CARBOHYD 81
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17315267"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17315267"
FT CROSSLNK 80
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 133 AA; 13058 MW; B5F04747F4655835 CRC64;
MQFSTVASIA AVAAVASAAA NVTTATVSQE STTLVTITSC EDHVCSETVS PALVSTATVT
VDDVITQYTT WCPLTTEAPK NGTSTAAPVT STEAPKNTTS AAPTHSVTSY TGAAAKALPA
AGALLAGAAA LLL
