CCW14_CANAL
ID CCW14_CANAL Reviewed; 234 AA.
AC Q5AFN8; A0A1D8PQP6; Q3MPP7;
DT 22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Covalently-linked cell wall protein 14;
DE Flags: Precursor;
GN Name=SSR1; Synonyms=CCW14; OrderedLocusNames=CAALFM_C700860WA;
GN ORFNames=CaO19.7030;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION, SUBCELLULAR LOCATION, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12904553; DOI=10.1099/mic.0.26301-0;
RA Garcera A., Martinez A.I., Castillo L., Elorza M.V., Sentandreu R.,
RA Valentin E.;
RT "Identification and study of a Candida albicans protein homologous to
RT Saccharomyces cerevisiae Ssr1p, an internal cell-wall protein.";
RL Microbiology 149:2137-2145(2003).
RN [5]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12734798; DOI=10.1002/yea.988;
RA Lee S.A., Wormsley S., Kamoun S., Lee A.F., Joiner K., Wong B.;
RT "An analysis of the Candida albicans genome database for soluble secreted
RT proteins using computer-based prediction algorithms.";
RL Yeast 20:595-610(2003).
RN [6]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=15302828; DOI=10.1128/ec.3.4.955-965.2004;
RA de Groot P.W., de Boer A.D., Cunningham J., Dekker H.L., de Jong L.,
RA Hellingwerf K.J., de Koster C., Klis F.M.;
RT "Proteomic analysis of Candida albicans cell walls reveals covalently bound
RT carbohydrate-active enzymes and adhesins.";
RL Eukaryot. Cell 3:955-965(2004).
RN [8]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=16024227; DOI=10.1016/j.resmic.2005.05.002;
RA Garcera A., Castillo L., Martinez A.I., Elorza M.V., Valentin E.,
RA Sentandreu R.;
RT "Anchorage of Candida albicans Ssr1 to the cell wall, and transcript
RT profiling of the null mutant.";
RL Res. Microbiol. 156:911-920(2005).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=17192409; DOI=10.1091/mbc.e06-10-0931;
RA Alvarez F.J., Konopka J.B.;
RT "Identification of an N-acetylglucosamine transporter that mediates hyphal
RT induction in Candida albicans.";
RL Mol. Biol. Cell 18:965-975(2007).
RN [10]
RP DISRUPTION PHENOTYPE.
RX PubMed=18765290; DOI=10.1016/j.fgb.2008.08.003;
RA Plaine A., Walker L., Da Costa G., Mora-Montes H.M., McKinnon A., Gow N.A.,
RA Gaillardin C., Munro C.A., Richard M.L.;
RT "Functional analysis of Candida albicans GPI-anchored proteins: roles in
RT cell wall integrity and caspofungin sensitivity.";
RL Fungal Genet. Biol. 45:1404-1414(2008).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=18227255; DOI=10.1099/mic.0.2007/012617-0;
RA Sosinska G.J., de Groot P.W., Teixeira de Mattos M.J., Dekker H.L.,
RA de Koster C.G., Hellingwerf K.J., Klis F.M.;
RT "Hypoxic conditions and iron restriction affect the cell-wall proteome of
RT Candida albicans grown under vagina-simulative conditions.";
RL Microbiology 154:510-520(2008).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=18712765; DOI=10.1002/pmic.200800110;
RA Castillo L., Calvo E., Martinez A.I., Ruiz-Herrera J., Valentin E.,
RA Lopez J.A., Sentandreu R.;
RT "A study of the Candida albicans cell wall proteome.";
RL Proteomics 8:3871-3881(2008).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=20641015; DOI=10.1002/yea.1775;
RA Sorgo A.G., Heilmann C.J., Dekker H.L., Brul S., de Koster C.G., Klis F.M.;
RT "Mass spectrometric analysis of the secretome of Candida albicans.";
RL Yeast 27:661-672(2010).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=21622905; DOI=10.1128/ec.05011-11;
RA Sorgo A.G., Heilmann C.J., Dekker H.L., Bekker M., Brul S., de Koster C.G.,
RA de Koning L.J., Klis F.M.;
RT "Effects of fluconazole on the secretome, the wall proteome, and wall
RT integrity of the clinical fungus Candida albicans.";
RL Eukaryot. Cell 10:1071-1081(2011).
RN [15]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=21602216; DOI=10.1099/mic.0.049395-0;
RA Heilmann C.J., Sorgo A.G., Siliakus A.R., Dekker H.L., Brul S.,
RA de Koster C.G., de Koning L.J., Klis F.M.;
RT "Hyphal induction in the human fungal pathogen Candida albicans reveals a
RT characteristic wall protein profile.";
RL Microbiology 157:2297-2307(2011).
CC -!- FUNCTION: Beta-glucan associated cell wall protein involved in cell
CC wall structure. May serve as cross-linking or coat-forming wall
CC protein. {ECO:0000269|PubMed:12904553, ECO:0000269|PubMed:16024227}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:12904553,
CC ECO:0000269|PubMed:15302828, ECO:0000269|PubMed:16024227,
CC ECO:0000269|PubMed:17192409, ECO:0000269|PubMed:18227255,
CC ECO:0000269|PubMed:18712765, ECO:0000269|PubMed:20641015,
CC ECO:0000269|PubMed:21602216, ECO:0000269|PubMed:21622905}. Membrane
CC {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. Note=Covalently-
CC linked GPI-modified cell wall protein (GPI-CWP).
CC -!- INDUCTION: Expressed in both yeast and hyphal cells at the same level,
CC indicating that it has no morphological differential expression.
CC Expression is slightly increased by fluconazole.
CC {ECO:0000269|PubMed:12904553, ECO:0000269|PubMed:21602216,
CC ECO:0000269|PubMed:21622905}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- DISRUPTION PHENOTYPE: Leads to increased sensitivity to caspofungin,
CC Congo red, calcofluor white, and zymolyase.
CC {ECO:0000269|PubMed:12904553, ECO:0000269|PubMed:18765290}.
CC -!- SIMILARITY: Belongs to the CCW14 family. {ECO:0000305}.
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DR EMBL; CP017629; AOW30462.1; -; Genomic_DNA.
DR RefSeq; XP_720324.1; XM_715231.1.
DR AlphaFoldDB; Q5AFN8; -.
DR STRING; 237561.Q5AFN8; -.
DR PRIDE; Q5AFN8; -.
DR GeneID; 3638055; -.
DR KEGG; cal:CAALFM_C700860WA; -.
DR CGD; CAL0000197579; SSR1.
DR VEuPathDB; FungiDB:C7_00860W_A; -.
DR eggNOG; ENOG502S1X2; Eukaryota.
DR HOGENOM; CLU_078308_1_0_1; -.
DR InParanoid; Q5AFN8; -.
DR OMA; NCYYSSR; -.
DR OrthoDB; 1612032at2759; -.
DR Proteomes; UP000000559; Chromosome 7.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IDA:CGD.
DR GO; GO:0005576; C:extracellular region; IDA:CGD.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:CGD.
DR GO; GO:0005886; C:plasma membrane; IDA:CGD.
DR GO; GO:0030445; C:yeast-form cell wall; IDA:CGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IEA:EnsemblFungi.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:CGD.
DR InterPro; IPR008427; Extracellular_membr_CFEM_dom.
DR Pfam; PF05730; CFEM; 1.
DR SMART; SM00747; CFEM; 1.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..215
FT /note="Covalently-linked cell wall protein 14"
FT /id="PRO_0000424853"
FT PROPEP 216..234
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424854"
FT REGION 86..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 215
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 234 AA; 22550 MW; 6125EA982CEB18BD CRC64;
MASFLKISTL IAIVSTLQTT LAAPPACLLA CVAKVEKGSK CSGLNDLSCI CTTKNSDVEK
CLKEICPNGD ADTAISAFKS SCSGYSSQSS SSESESESAS SEESSASASA SASSSAGKSS
NVEASTTKES SSAKASSSAA GSSEAVSSAT ETASTEESSS AAASASASAS ATKESSSEAA
SSTSSTLKES KTSTTAAASS SESTTATGVL TQSEGSAAKV GLGALVGLVG AVLL
