ID   CCZ1A_ARATH             Reviewed;         515 AA.
AC   F4I2S4; Q8L8Y5; Q9S9N3;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Vacuolar fusion protein CCZ1 homolog A {ECO:0000305};
GN   Name=CCZ1A {ECO:0000303|PubMed:24824487};
GN   OrderedLocusNames=At1g16020 {ECO:0000312|Araport:AT1G16020};
GN   ORFNames=T24D18.12 {ECO:0000312|EMBL:AAF18499.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-515 (ISOFORM 2).
RA   Wrobel R.L., Kimball T.L., Steffen E., Thao S., Aceti D.J., Blommel P.G.,
RA   Newman C.S., Zhao Q., Fox B.G., Phillips G.N. Jr., Markley J.L.;
RT   "Arabidopsis thaliana At1g16020 mRNA sequence.";
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, INTERACTION WITH MON1, AND SUBCELLULAR LOCATION.
RX   PubMed=24824487; DOI=10.1105/tpc.114.123141;
RA   Cui Y., Zhao Q., Gao C., Ding Y., Zeng Y., Ueda T., Nakano A., Jiang L.;
RT   "Activation of the Rab7 GTPase by the MON1-CCZ1 complex is essential for
RT   PVC-to-vacuole trafficking and plant growth in Arabidopsis.";
RL   Plant Cell 26:2080-2097(2014).
CC   -!- FUNCTION: Plays an important role in membrane trafficking through the
CC       secretory apparatus. In complex with MON1, acts as a guanine exchange
CC       factor (GEF) for RABG3F of the Rab7 protein family. Promotes the
CC       exchange of GDP to GTP, converting RABG3F from an inactive GDP-bound
CC       form into an active GTP-bound form. The RABG3F active form is involved
CC       in protein trafficking from prevacuolar compartments (PVCs) to
CC       vacuoles. May serve as a linker between Rab5 and Rab7 protein families
CC       in PVCs and mediate PVC maturation. {ECO:0000269|PubMed:24824487}.
CC   -!- SUBUNIT: Interacts with MON1. {ECO:0000269|PubMed:24824487}.
CC   -!- SUBCELLULAR LOCATION: Endosome {ECO:0000269|PubMed:24824487}.
CC       Prevacuolar compartment {ECO:0000269|PubMed:24824487}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=F4I2S4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=F4I2S4-2; Sequence=VSP_058666;
CC   -!- SIMILARITY: Belongs to the CCZ1 family. {ECO:0000305}.
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DR   EMBL; AC010924; AAF18499.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE29398.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE29399.1; -; Genomic_DNA.
DR   EMBL; AY088734; AAM67052.1; -; mRNA.
DR   EMBL; BT012033; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; H86294; H86294.
DR   RefSeq; NP_563989.1; NM_101470.3. [F4I2S4-1]
DR   RefSeq; NP_849674.1; NM_179343.2. [F4I2S4-2]
DR   AlphaFoldDB; F4I2S4; -.
DR   SMR; F4I2S4; -.
DR   STRING; 3702.AT1G16020.1; -.
DR   iPTMnet; F4I2S4; -.
DR   PaxDb; F4I2S4; -.
DR   PRIDE; F4I2S4; -.
DR   ProteomicsDB; 223876; -. [F4I2S4-1]
DR   DNASU; 838172; -.
DR   EnsemblPlants; AT1G16020.1; AT1G16020.1; AT1G16020. [F4I2S4-1]
DR   EnsemblPlants; AT1G16020.2; AT1G16020.2; AT1G16020. [F4I2S4-2]
DR   GeneID; 838172; -.
DR   Gramene; AT1G16020.1; AT1G16020.1; AT1G16020. [F4I2S4-1]
DR   Gramene; AT1G16020.2; AT1G16020.2; AT1G16020. [F4I2S4-2]
DR   KEGG; ath:AT1G16020; -.
DR   Araport; AT1G16020; -.
DR   TAIR; locus:2200547; AT1G16020.
DR   eggNOG; KOG2622; Eukaryota.
DR   HOGENOM; CLU_037828_2_0_1; -.
DR   InParanoid; F4I2S4; -.
DR   OMA; FLRAQNF; -.
DR   OrthoDB; 448107at2759; -.
DR   PRO; PR:F4I2S4; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I2S4; baseline and differential.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0035658; C:Mon1-Ccz1 complex; IEA:InterPro.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   InterPro; IPR013176; Ccz1.
DR   InterPro; IPR043987; CCZ1/INTU/HSP4_longin_1.
DR   PANTHER; PTHR13056; PTHR13056; 1.
DR   Pfam; PF19031; Intu_longin_1; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Endosome; Guanine-nucleotide releasing factor;
KW   Reference proteome.
FT   CHAIN           1..515
FT                   /note="Vacuolar fusion protein CCZ1 homolog A"
FT                   /id="PRO_0000438481"
FT   VAR_SEQ         152..165
FT                   /note="TFQIWSLSEDCCCE -> K (in isoform 2)"
FT                   /id="VSP_058666"
FT   CONFLICT        93
FT                   /note="E -> G (in Ref. 4; BT012033)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        252
FT                   /note="L -> F (in Ref. 3; AAM67052)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   515 AA;  57840 MW;  BE340A8CCC0EF2C9 CRC64;
     MASMSSGDES LRLCMFDLRR GQTEGQELEK ILFFYPADLD FSTQLSVIGL SEGLITFTRL
     FSPEAACEVI EAERHSHVFY EAEPDIWMVM VVEKNKETGA IWRIDALRRV LKEVHSLFVM
     FHGSIRALIE KEPTGGLTRS LLYPFITDYL STFQIWSLSE DCCCEFFVGK KLQLPTFRET
     LRERGTVQML TLARDTAVEV QSLVQVLDSC AGSLRCHSMI LFQDLLVSTT LSADDTVDLF
     TFAVMRLTSK ALSSDTSSWS YLRKGPGSSE ISSRSNLAPV GSIDSLHSRN GNNMHHVIRP
     LQNDKWTKGK DGFLITDIWG LETGGSPDSA IPTIWLQQTQ ERMYLLAYQH KSLTLLLLMP
     TNAIVNGDLS ISAVKQQVIE DASLRILKIE ENISRGWGGE NAYHIKGYRY LVVDNDTKVS
     RSSPSGKVTT LAKESLLALN KLREEVDSEK SRAKGQEKDM EICIRAKNNV WVIARVTRGK
     ELYMALEKGS DTLLDTTDAV GRFSNRYCSG AFLMD