CCZ1B_ARATH
ID CCZ1B_ARATH Reviewed; 497 AA.
AC C0Z274; B9DGZ2; Q9SAG9;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Vacuolar fusion protein CCZ1 homolog B {ECO:0000305};
GN Name=CCZ1B {ECO:0000303|PubMed:24824487};
GN OrderedLocusNames=At1g80910 {ECO:0000312|Araport:AT1G80910};
GN ORFNames=F23A5.27 {ECO:0000312|EMBL:AAF14679.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP INTERACTION WITH MON1.
RX PubMed=24824487; DOI=10.1105/tpc.114.123141;
RA Cui Y., Zhao Q., Gao C., Ding Y., Zeng Y., Ueda T., Nakano A., Jiang L.;
RT "Activation of the Rab7 GTPase by the MON1-CCZ1 complex is essential for
RT PVC-to-vacuole trafficking and plant growth in Arabidopsis.";
RL Plant Cell 26:2080-2097(2014).
CC -!- FUNCTION: Plays an important role in membrane trafficking through the
CC secretory apparatus. In complex with MON1, acts as a guanine exchange
CC factor (GEF) for RABG3F of the RAB7 protein family. Promotes the
CC exchange of GDP to GTP, converting RABG3F from an inactive GDP-bound
CC form into an active GTP-bound form. The RABG3F active form is involved
CC in protein trafficking from prevacuolar compartments (PVCs) to
CC vacuoles. May serve as a linker between Rab5 and Rab7 protein families
CC in PVCs and mediate PVC maturation. {ECO:0000250|UniProtKB:F4I2S4}.
CC -!- SUBUNIT: Interacts with MON1. {ECO:0000269|PubMed:24824487}.
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250|UniProtKB:F4I2S4}.
CC Prevacuolar compartment {ECO:0000250|UniProtKB:F4I2S4}.
CC -!- SIMILARITY: Belongs to the CCZ1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF14679.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011713; AAF14679.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36466.1; -; Genomic_DNA.
DR EMBL; AK317335; BAH20009.1; -; mRNA.
DR EMBL; AK318688; BAH56803.1; -; mRNA.
DR PIR; A96842; A96842.
DR RefSeq; NP_178206.1; NM_106739.4.
DR AlphaFoldDB; C0Z274; -.
DR SMR; C0Z274; -.
DR IntAct; C0Z274; 4.
DR STRING; 3702.AT1G80910.1; -.
DR iPTMnet; C0Z274; -.
DR PaxDb; C0Z274; -.
DR PRIDE; C0Z274; -.
DR ProteomicsDB; 223877; -.
DR DNASU; 844431; -.
DR EnsemblPlants; AT1G80910.1; AT1G80910.1; AT1G80910.
DR GeneID; 844431; -.
DR Gramene; AT1G80910.1; AT1G80910.1; AT1G80910.
DR KEGG; ath:AT1G80910; -.
DR Araport; AT1G80910; -.
DR TAIR; locus:2025782; AT1G80910.
DR eggNOG; KOG2622; Eukaryota.
DR HOGENOM; CLU_037828_1_1_1; -.
DR InParanoid; C0Z274; -.
DR OMA; WVIARLN; -.
DR OrthoDB; 448107at2759; -.
DR PRO; PR:C0Z274; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; C0Z274; baseline and differential.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0035658; C:Mon1-Ccz1 complex; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR013176; Ccz1.
DR InterPro; IPR043987; CCZ1/INTU/HSP4_longin_1.
DR PANTHER; PTHR13056; PTHR13056; 1.
DR Pfam; PF19031; Intu_longin_1; 1.
PE 1: Evidence at protein level;
KW Endosome; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..497
FT /note="Vacuolar fusion protein CCZ1 homolog B"
FT /id="PRO_0000438482"
FT REGION 244..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 460
FT /note="R -> G (in Ref. 3; BAH20009)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 55429 MW; 394CF4DA7EC9695E CRC64;
MGMASMSSGT ESLRLCVFDL RRGQHEGQEL DKILFFYPPD LTFSTQLSVI GLSEGLITFT
RLFSPEAACE VIEAERHSHV FYEAEPDIWM VMIVEKNKEI EAVWRIDALR RVLKEVHSLF
VMFQGSIRAL LEKEPTGGLV RSHLYPFITD YLNDLFVGKK QQLPSFRDTL KERGTVQMLT
LARDAALEVQ SLVGVLDSCA GTVRCHSVIL FHDLLVSTTL SPDDTVDLFA FSVMRLTTNA
LSSGTSSWSY LRKGSGSPQI SSRSTTVPPL GSGGTLPSGN GSSTGRVIRP LQHDKWSKGK
DGFLVTDIWG LDATPTILIQ KTQESFYLLT YQYKSLTLVL LVPIAAIVNG ELDISFVKQQ
VIENASTKIL KVEEKLSKGW GGENAYHVSG YRYLLVDNDM EVSRASPPGK VATLAKESLL
ALNKLREEVD TEKNRSKQEK DMEICIRAKN NTWVIARLNR GKELYMALEK ASETLLDATD
SVQRFSNRYC SGAFPMD
