CCZ1_MOUSE
ID CCZ1_MOUSE Reviewed; 480 AA.
AC Q8C1Y8; Q3U891;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Vacuolar fusion protein CCZ1 homolog;
GN Name=Ccz1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts in concert with MON1A, as a guanine exchange factor
CC (GEF) for RAB7, promotes the exchange of GDP to GTP, converting it from
CC an inactive GDP-bound form into an active GTP-bound form.
CC {ECO:0000250|UniProtKB:P86791}.
CC -!- SUBUNIT: Interacts with MON1A. Found in a complex with RMC1, CCZ1 MON1A
CC and MON1B. {ECO:0000250|UniProtKB:P86791}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CCZ1 family. {ECO:0000305}.
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DR EMBL; AK090008; BAC41041.1; -; mRNA.
DR EMBL; AK150793; BAE29856.1; -; mRNA.
DR EMBL; AK152328; BAE31127.1; -; mRNA.
DR EMBL; AK152576; BAE31327.1; -; mRNA.
DR EMBL; AK153160; BAE31770.1; -; mRNA.
DR EMBL; BC048077; AAH48077.1; -; mRNA.
DR CCDS; CCDS19846.1; -.
DR RefSeq; NP_808350.1; NM_177682.3.
DR AlphaFoldDB; Q8C1Y8; -.
DR SMR; Q8C1Y8; -.
DR BioGRID; 231188; 7.
DR STRING; 10090.ENSMUSP00000031621; -.
DR iPTMnet; Q8C1Y8; -.
DR PhosphoSitePlus; Q8C1Y8; -.
DR SwissPalm; Q8C1Y8; -.
DR EPD; Q8C1Y8; -.
DR jPOST; Q8C1Y8; -.
DR MaxQB; Q8C1Y8; -.
DR PaxDb; Q8C1Y8; -.
DR PeptideAtlas; Q8C1Y8; -.
DR PRIDE; Q8C1Y8; -.
DR ProteomicsDB; 281430; -.
DR DNASU; 231874; -.
DR Ensembl; ENSMUST00000031621; ENSMUSP00000031621; ENSMUSG00000029617.
DR GeneID; 231874; -.
DR KEGG; mmu:231874; -.
DR UCSC; uc009ale.1; mouse.
DR CTD; 51622; -.
DR MGI; MGI:2141070; Ccz1.
DR VEuPathDB; HostDB:ENSMUSG00000029617; -.
DR eggNOG; KOG2622; Eukaryota.
DR GeneTree; ENSGT00390000004713; -.
DR HOGENOM; CLU_037828_2_0_1; -.
DR InParanoid; Q8C1Y8; -.
DR OMA; FLRAQNF; -.
DR OrthoDB; 448107at2759; -.
DR PhylomeDB; Q8C1Y8; -.
DR TreeFam; TF314962; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 231874; 10 hits in 73 CRISPR screens.
DR ChiTaRS; Ccz1; mouse.
DR PRO; PR:Q8C1Y8; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8C1Y8; protein.
DR Bgee; ENSMUSG00000029617; Expressed in medial ganglionic eminence and 271 other tissues.
DR Genevisible; Q8C1Y8; MM.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0035658; C:Mon1-Ccz1 complex; ISS:UniProtKB.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR013176; Ccz1.
DR InterPro; IPR043987; CCZ1/INTU/HSP4_longin_1.
DR InterPro; IPR043989; CCZ1/INTU/HSP4_longin_3.
DR InterPro; IPR043988; CCZ1/INTU_longin_2.
DR PANTHER; PTHR13056; PTHR13056; 1.
DR Pfam; PF19031; Intu_longin_1; 1.
DR Pfam; PF19032; Intu_longin_2; 1.
DR Pfam; PF19033; Intu_longin_3; 1.
PE 1: Evidence at protein level;
KW Acetylation; Guanine-nucleotide releasing factor; Lysosome; Membrane;
KW Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P86791"
FT CHAIN 2..480
FT /note="Vacuolar fusion protein CCZ1 homolog"
FT /id="PRO_0000089585"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P86791"
FT MOD_RES 74
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P86791"
FT MOD_RES 264
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
SQ SEQUENCE 480 AA; 55505 MW; B6ABFCE751942389 CRC64;
MAAAAAGPGA WAAQEKQFPP ALLSFFIYNP RFGPREGEEE NKILFYHPNE VEKNEKIRNV
GLCEAIVQFT RTFSPSKPAK SLHTQKNRQF FNEPEENFWM VMVVRNPIIE KQSKDGKAVV
EYQEEELLDK VYSSVLQQCY SMYKLFNGTF LKAMEDGGVK LLKERLEKFF HRYLQTLHLQ
SCDLLDIFGG ISFFPLDKMT YLKIQSFINR MEESLSVVKY TAFLYNDQLI WSGLEQDDMR
ILYKYLTTSL FPRHIEPELA GRDSPVRAEM PGNLQHYGRF LTGPLNLNDP EAKCRFPKIF
VNTDDTYEEL HLIVYKAMSA AVCFMIDAST PLTLDFCRRL DSIVGPQLTV LASDICEQFN
INKRISGSEK EPQFKFIYFN HMNLAEKSTI HMRKTPSVSL TSVHPDLMKI LGDINSDFTR
ADEDEEIIVK AMSDYWVVGK KSDQRELYVI LSQKNANLIE VNEEVKKLCA TQFNNIFFLD
