CD003_HUMAN
ID CD003_HUMAN Reviewed; 66 AA.
AC Q8WVX3; Q6J203;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Uncharacterized protein C4orf3;
DE AltName: Full=Hepatitis C virus F protein-transactivated protein 1;
DE Short=HCV F-transactivated protein 1;
GN Name=C4orf3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=16174453;
RA Guo J., Cheng J., Ji D., Zhao L.F., Gao X.S., Liu Y., Wu S.H.;
RT "Screening and cloning target genes transactivated by hepatitis C virus F
RT protein using suppression subtractive hybridization technique.";
RL Zhonghua Gan Zang Bing Za Zhi 13:660-663(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT GLN-17.
RC TISSUE=Bone marrow;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- INTERACTION:
CC Q8WVX3-2; Q86Y34: ADGRG3; NbExp=3; IntAct=EBI-12003442, EBI-17979264;
CC Q8WVX3-2; Q13520: AQP6; NbExp=3; IntAct=EBI-12003442, EBI-13059134;
CC Q8WVX3-2; Q3SXY8: ARL13B; NbExp=3; IntAct=EBI-12003442, EBI-11343438;
CC Q8WVX3-2; Q9BXK5: BCL2L13; NbExp=5; IntAct=EBI-12003442, EBI-747430;
CC Q8WVX3-2; Q13323: BIK; NbExp=3; IntAct=EBI-12003442, EBI-700794;
CC Q8WVX3-2; O00421-2: CCRL2; NbExp=3; IntAct=EBI-12003442, EBI-12874086;
CC Q8WVX3-2; P11912: CD79A; NbExp=3; IntAct=EBI-12003442, EBI-7797864;
CC Q8WVX3-2; Q8N5K1: CISD2; NbExp=3; IntAct=EBI-12003442, EBI-1045797;
CC Q8WVX3-2; O95484: CLDN9; NbExp=3; IntAct=EBI-12003442, EBI-18341636;
CC Q8WVX3-2; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-12003442, EBI-6942903;
CC Q8WVX3-2; P25025: CXCR2; NbExp=3; IntAct=EBI-12003442, EBI-2835281;
CC Q8WVX3-2; Q9BQA9: CYBC1; NbExp=3; IntAct=EBI-12003442, EBI-2680384;
CC Q8WVX3-2; O15121: DEGS1; NbExp=3; IntAct=EBI-12003442, EBI-1052713;
CC Q8WVX3-2; Q15125: EBP; NbExp=3; IntAct=EBI-12003442, EBI-3915253;
CC Q8WVX3-2; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-12003442, EBI-781551;
CC Q8WVX3-2; Q8TBP5: FAM174A; NbExp=3; IntAct=EBI-12003442, EBI-18636064;
CC Q8WVX3-2; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-12003442, EBI-18304435;
CC Q8WVX3-2; O15552: FFAR2; NbExp=3; IntAct=EBI-12003442, EBI-2833872;
CC Q8WVX3-2; P36382: GJA5; NbExp=3; IntAct=EBI-12003442, EBI-750433;
CC Q8WVX3-2; Q9NTQ9: GJB4; NbExp=3; IntAct=EBI-12003442, EBI-12831526;
CC Q8WVX3-2; O95377: GJB5; NbExp=3; IntAct=EBI-12003442, EBI-3909454;
CC Q8WVX3-2; Q9BZJ8: GPR61; NbExp=3; IntAct=EBI-12003442, EBI-12808020;
CC Q8WVX3-2; Q9HCP6: HHATL; NbExp=3; IntAct=EBI-12003442, EBI-5916693;
CC Q8WVX3-2; Q8N5M9: JAGN1; NbExp=3; IntAct=EBI-12003442, EBI-10266796;
CC Q8WVX3-2; Q13571: LAPTM5; NbExp=3; IntAct=EBI-12003442, EBI-2865663;
CC Q8WVX3-2; O95867: LY6G6C; NbExp=3; IntAct=EBI-12003442, EBI-9088345;
CC Q8WVX3-2; Q5SR56: MFSD14B; NbExp=3; IntAct=EBI-12003442, EBI-373355;
CC Q8WVX3-2; Q7Z6M3: MILR1; NbExp=3; IntAct=EBI-12003442, EBI-18391669;
CC Q8WVX3-2; Q15546: MMD; NbExp=3; IntAct=EBI-12003442, EBI-17873222;
CC Q8WVX3-2; Q8N4V1: MMGT1; NbExp=3; IntAct=EBI-12003442, EBI-6163737;
CC Q8WVX3-2; Q9HC36: MRM3; NbExp=3; IntAct=EBI-12003442, EBI-1045440;
CC Q8WVX3-2; Q96HJ5: MS4A3; NbExp=3; IntAct=EBI-12003442, EBI-12806656;
CC Q8WVX3-2; Q6IBW4-4: NCAPH2; NbExp=3; IntAct=EBI-12003442, EBI-10247000;
CC Q8WVX3-2; Q53GL0: PLEKHO1; NbExp=3; IntAct=EBI-12003442, EBI-949945;
CC Q8WVX3-2; O14684: PTGES; NbExp=3; IntAct=EBI-12003442, EBI-11161398;
CC Q8WVX3-2; Q9H6H4: REEP4; NbExp=3; IntAct=EBI-12003442, EBI-7545592;
CC Q8WVX3-2; Q86VR2: RETREG3; NbExp=3; IntAct=EBI-12003442, EBI-10192441;
CC Q8WVX3-2; O75783: RHBDL1; NbExp=3; IntAct=EBI-12003442, EBI-12104986;
CC Q8WVX3-2; Q9UBD6: RHCG; NbExp=3; IntAct=EBI-12003442, EBI-15853497;
CC Q8WVX3-2; Q3SXP7: SHISAL1; NbExp=3; IntAct=EBI-12003442, EBI-18037857;
CC Q8WVX3-2; Q15849: SLC14A2; NbExp=3; IntAct=EBI-12003442, EBI-1573290;
CC Q8WVX3-2; Q9BZV2: SLC19A3; NbExp=3; IntAct=EBI-12003442, EBI-3923779;
CC Q8WVX3-2; Q9NQQ7-3: SLC35C2; NbExp=3; IntAct=EBI-12003442, EBI-17295964;
CC Q8WVX3-2; Q7Z769: SLC35E3; NbExp=3; IntAct=EBI-12003442, EBI-13389236;
CC Q8WVX3-2; Q9NP94: SLC39A2; NbExp=3; IntAct=EBI-12003442, EBI-12898013;
CC Q8WVX3-2; Q96GZ6: SLC41A3; NbExp=3; IntAct=EBI-12003442, EBI-7225508;
CC Q8WVX3-2; Q9UIG8-2: SLCO3A1; NbExp=3; IntAct=EBI-12003442, EBI-13041931;
CC Q8WVX3-2; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-12003442, EBI-17280858;
CC Q8WVX3-2; Q16623: STX1A; NbExp=3; IntAct=EBI-12003442, EBI-712466;
CC Q8WVX3-2; Q8TBG9: SYNPR; NbExp=3; IntAct=EBI-12003442, EBI-10273251;
CC Q8WVX3-2; Q9NPL8: TIMMDC1; NbExp=3; IntAct=EBI-12003442, EBI-6268651;
CC Q8WVX3-2; Q96DZ7: TM4SF19; NbExp=3; IntAct=EBI-12003442, EBI-6448756;
CC Q8WVX3-2; Q96IK0: TMEM101; NbExp=3; IntAct=EBI-12003442, EBI-3922699;
CC Q8WVX3-2; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-12003442, EBI-10982110;
CC Q8WVX3-2; Q9NWD8: TMEM248; NbExp=3; IntAct=EBI-12003442, EBI-10314986;
CC Q8WVX3-2; Q9NWC5: TMEM45A; NbExp=3; IntAct=EBI-12003442, EBI-10823938;
CC Q8WVX3-2; Q96B21: TMEM45B; NbExp=3; IntAct=EBI-12003442, EBI-3923061;
CC Q8WVX3-2; Q9NW97: TMEM51; NbExp=3; IntAct=EBI-12003442, EBI-726044;
CC Q8WVX3-2; O95070: YIF1A; NbExp=3; IntAct=EBI-12003442, EBI-2799703;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8WVX3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8WVX3-2; Sequence=VSP_032405;
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DR EMBL; AY605045; AAT35812.1; -; mRNA.
DR EMBL; AC092656; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC017399; AAH17399.1; -; mRNA.
DR CCDS; CCDS43266.1; -. [Q8WVX3-1]
DR CCDS; CCDS54798.1; -. [Q8WVX3-2]
DR RefSeq; NP_001001701.2; NM_001001701.3. [Q8WVX3-1]
DR RefSeq; NP_001163801.1; NM_001170330.1. [Q8WVX3-2]
DR AlphaFoldDB; Q8WVX3; -.
DR SMR; Q8WVX3; -.
DR BioGRID; 134952; 86.
DR IntAct; Q8WVX3; 68.
DR MINT; Q8WVX3; -.
DR STRING; 9606.ENSP00000382026; -.
DR TCDB; 1.A.50.6.1; the phospholamban (ca(2+)-channel and ca(2+)-atpase regulator) (plb) family.
DR GlyGen; Q8WVX3; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8WVX3; -.
DR PhosphoSitePlus; Q8WVX3; -.
DR BioMuta; C4orf3; -.
DR EPD; Q8WVX3; -.
DR jPOST; Q8WVX3; -.
DR MassIVE; Q8WVX3; -.
DR MaxQB; Q8WVX3; -.
DR PaxDb; Q8WVX3; -.
DR PeptideAtlas; Q8WVX3; -.
DR PRIDE; Q8WVX3; -.
DR ProteomicsDB; 74831; -. [Q8WVX3-1]
DR ProteomicsDB; 74832; -. [Q8WVX3-2]
DR TopDownProteomics; Q8WVX3-1; -. [Q8WVX3-1]
DR Antibodypedia; 26668; 97 antibodies from 14 providers.
DR DNASU; 401152; -.
DR Ensembl; ENST00000399075.6; ENSP00000382026.4; ENSG00000164096.13. [Q8WVX3-2]
DR Ensembl; ENST00000504110.2; ENSP00000485697.1; ENSG00000164096.13. [Q8WVX3-1]
DR GeneID; 401152; -.
DR KEGG; hsa:401152; -.
DR MANE-Select; ENST00000504110.2; ENSP00000485697.1; NM_001001701.4; NP_001001701.2.
DR UCSC; uc003icv.5; human. [Q8WVX3-1]
DR CTD; 401152; -.
DR DisGeNET; 401152; -.
DR GeneCards; C4orf3; -.
DR HGNC; HGNC:19225; C4orf3.
DR HPA; ENSG00000164096; Low tissue specificity.
DR neXtProt; NX_Q8WVX3; -.
DR OpenTargets; ENSG00000164096; -.
DR PharmGKB; PA164717306; -.
DR VEuPathDB; HostDB:ENSG00000164096; -.
DR eggNOG; ENOG502T0GP; Eukaryota.
DR GeneTree; ENSGT00530000064570; -.
DR HOGENOM; CLU_135965_0_0_1; -.
DR InParanoid; Q8WVX3; -.
DR OMA; DRFPKHS; -.
DR OrthoDB; 1307860at2759; -.
DR PhylomeDB; Q8WVX3; -.
DR TreeFam; TF343305; -.
DR PathwayCommons; Q8WVX3; -.
DR SignaLink; Q8WVX3; -.
DR BioGRID-ORCS; 401152; 6 hits in 1068 CRISPR screens.
DR ChiTaRS; C4orf3; human.
DR GenomeRNAi; 401152; -.
DR Pharos; Q8WVX3; Tbio.
DR PRO; PR:Q8WVX3; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q8WVX3; protein.
DR Bgee; ENSG00000164096; Expressed in endothelial cell and 196 other tissues.
DR Genevisible; Q8WVX3; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:1901895; P:negative regulation of ATPase-coupled calcium transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:1901877; P:negative regulation of calcium ion binding; IEA:Ensembl.
DR InterPro; IPR038780; C4orf3.
DR PANTHER; PTHR37367; PTHR37367; 1.
DR Pfam; PF17696; DUF5542; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..66
FT /note="Uncharacterized protein C4orf3"
FT /id="PRO_0000325784"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT VAR_SEQ 1
FT /note="M -> MTSLINSPINRRPLQNVEGNNRCQRKAKNYGNKYFIHCLDLEKITLS
FT PRRKHDIEGGDKLNVKFSQLRSRRQRKAEPGACALGRVGSECIPEPGARRTAQAAGLRS
FT VSGAANTKVRELKHFRFLGLLRSCRSEM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16174453"
FT /id="VSP_032405"
FT VARIANT 17
FT /note="R -> Q (in dbSNP:rs17851522)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_039911"
FT VARIANT 32
FT /note="P -> H (in dbSNP:rs11544530)"
FT /id="VAR_039912"
SQ SEQUENCE 66 AA; 7604 MW; 6973371B80AE75CC CRC64;
MEVDAPGVDG RDGLRERRGF SEGGRQNFDV RPQSGANGLP KHSYWLDLWL FILFDVVVFL
FVYFLP
