CD27A_ARATH
ID CD27A_ARATH Reviewed; 717 AA.
AC Q06AN9; O04325; Q9LU16;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 2.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Cell division cycle protein 27 homolog A;
DE Short=CDC27 homolog A;
DE AltName: Full=Anaphase-promoting complex subunit 3;
GN Name=CDC27A; Synonyms=APC3; OrderedLocusNames=At3g16320;
GN ORFNames=T02O04.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16940752; DOI=10.4161/cc.5.17.3125;
RA Eloy N.B., Coppens F., Beemster G.T., Hemerly A.S., Ferreira P.C.;
RT "The Arabidopsis anaphase promoting complex (APC): regulation through
RT subunit availability in plant tissues.";
RL Cell Cycle 5:1957-1965(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH APC2 AND APC10.
RX PubMed=17944809; DOI=10.1111/j.1365-313x.2007.03312.x;
RA Perez-Perez J.M., Serralbo O., Vanstraelen M., Gonzalez C., Criqui M.C.,
RA Genschik P., Kondorosi E., Scheres B.;
RT "Specialization of CDC27 function in the Arabidopsis thaliana anaphase-
RT promoting complex (APC/C).";
RL Plant J. 53:78-89(2008).
RN [6]
RP FUNCTION.
RX PubMed=19629716; DOI=10.1007/s11103-009-9525-7;
RA Rojas C.A., Eloy N.B., Lima Mde F., Rodrigues R.L., Franco L.O.,
RA Himanen K., Beemster G.T., Hemerly A.S., Ferreira P.C.;
RT "Overexpression of the Arabidopsis anaphase promoting complex subunit
RT CDC27a increases growth rate and organ size.";
RL Plant Mol. Biol. 71:307-318(2009).
CC -!- FUNCTION: Component of the anaphase promoting complex/cyclosome
CC (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex
CC that controls progression through mitosis and the G1 phase of the cell
CC cycle. The APC/C complex controls several key steps in the cell cycle
CC by mediating ubiquitination and subsequent degradation of target
CC proteins such as cyclins. The APC/C complex is required for the female
CC gametophyte development and is involved in several aspect of
CC development by controlling cell division and cell elongation. Involved
CC in the control of endoreduplication. Functionally redundant with CDC27B
CC in the control of gametophyte development.
CC {ECO:0000269|PubMed:17944809, ECO:0000269|PubMed:19629716}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: The APC/C is composed of at least 10 subunits. Interacts with
CC APC2 and APC10. {ECO:0000269|PubMed:17944809}.
CC -!- INTERACTION:
CC Q06AN9; Q9ZPW2: APC10; NbExp=3; IntAct=EBI-1749373, EBI-1749354;
CC Q06AN9; Q8H1U5: APC2; NbExp=2; IntAct=EBI-1749373, EBI-1749410;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Cdc27a and cdc27b double
CC mutant is gametophytic lethal (PubMed:17944809).
CC {ECO:0000269|PubMed:17944809}.
CC -!- SIMILARITY: Belongs to the APC3/CDC27 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63645.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAB01271.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ922637; ABI97116.1; -; mRNA.
DR EMBL; AB023046; BAB01271.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC001645; AAB63645.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; ANM63407.1; -; Genomic_DNA.
DR RefSeq; NP_188253.3; NM_112503.5.
DR AlphaFoldDB; Q06AN9; -.
DR SMR; Q06AN9; -.
DR BioGRID; 6213; 5.
DR IntAct; Q06AN9; 4.
DR STRING; 3702.AT3G16320.1; -.
DR PaxDb; Q06AN9; -.
DR PRIDE; Q06AN9; -.
DR EnsemblPlants; AT3G16320.2; AT3G16320.2; AT3G16320.
DR GeneID; 820879; -.
DR Gramene; AT3G16320.2; AT3G16320.2; AT3G16320.
DR KEGG; ath:AT3G16320; -.
DR Araport; AT3G16320; -.
DR TAIR; locus:2094917; AT3G16320.
DR eggNOG; KOG1126; Eukaryota.
DR HOGENOM; CLU_008850_1_1_1; -.
DR InParanoid; Q06AN9; -.
DR OMA; MCKYDCY; -.
DR PhylomeDB; Q06AN9; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q06AN9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q06AN9; baseline and differential.
DR Genevisible; Q06AN9; AT.
DR GO; GO:0005680; C:anaphase-promoting complex; IPI:TAIR.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031145; P:anaphase-promoting complex-dependent catabolic process; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IBA:GO_Central.
DR GO; GO:0007091; P:metaphase/anaphase transition of mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0045842; P:positive regulation of mitotic metaphase/anaphase transition; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR013105; TPR_2.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF00515; TPR_1; 1.
DR Pfam; PF07719; TPR_2; 1.
DR SMART; SM00028; TPR; 7.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50005; TPR; 8.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome; Repeat;
KW TPR repeat; Ubl conjugation pathway.
FT CHAIN 1..717
FT /note="Cell division cycle protein 27 homolog A"
FT /id="PRO_0000396839"
FT REPEAT 421..454
FT /note="TPR 1"
FT REPEAT 489..522
FT /note="TPR 2"
FT REPEAT 524..556
FT /note="TPR 3"
FT REPEAT 557..590
FT /note="TPR 4"
FT REPEAT 592..624
FT /note="TPR 5"
FT REPEAT 625..658
FT /note="TPR 6"
FT REPEAT 659..692
FT /note="TPR 7"
FT REGION 198..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 342..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..356
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 164
FT /note="Missing (in Ref. 1; ABI97116)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="L -> P (in Ref. 1; ABI97116)"
FT /evidence="ECO:0000305"
FT CONFLICT 355
FT /note="I -> T (in Ref. 1; ABI97116)"
FT /evidence="ECO:0000305"
FT CONFLICT 387
FT /note="L -> I (in Ref. 1; ABI97116)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 717 AA; 81114 MW; 9D060B9291ED37A3 CRC64;
MMENLLANCV QKNLNHFMFT NAIFLCELLL AQFPSEVNLQ LLARCYLSNS QAYSAYYILK
GSKTPQSRYL FAFSCFKLDL LGEAEAALLP CEDYAEEVPG GAAGHYLLGL IYRYSGRKNC
SIQQFRMALS FDPLCWEAYG ELCSLGAAEE ASTVFGNVAS QRLQKTCVEQ RISFSEGATI
DQITDSDKAL KDTGLSQTEH IPGENQQDLK IMQQPGDIPP NTDRQLSTNG WDLNTPSPVL
LQVMDALPPL LLKNMRRPAV EGSLMSVHGV RVRRRNFFSE ELSAEAQEES GRRRSARIAA
RKKNPMSQSF GKDSHWLHLS PSESNYAPSL SSMIGKCRIQ SSKEATTSGQ SVSDIGSSVD
DEEKSNPSES SPDRFSLISG ISEVLSLLKI LGDGHRHLHM YKCQEALLAY QKLSQKQYNT
HWVLMQVGKA YFELQDYFNA DSSFTLAHQK YPYALEGMDT YSTVLYHLKE EMRLGYLAQE
LISVDRLSPE SWCAVGNCYS LRKDHDTALK MFQRAIQLNE RFTYAHTLCG HEFAALEEFE
DAERCYRKAL GIDTRHYNAW YGLGMTYLRQ EKFEFAQHQF QLALQINPRS SVIMCYYGIA
LHESKRNDEA LMMMEKAVLT DAKNPLPKYY KAHILTSLGD YHKAQKVLEE LKECAPQESS
VHASLGKIYN QLKQYDKAVL HFGIALDLSP SPSDAVKIKA YMERLILPDE LVTEENL
