CD28_MOUSE
ID CD28_MOUSE Reviewed; 218 AA.
AC P31041; Q6GSH7;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=T-cell-specific surface glycoprotein CD28;
DE AltName: CD_antigen=CD28;
DE Flags: Precursor;
GN Name=Cd28;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2157764;
RA Gross J.A., St John T., Allison J.P.;
RT "The murine homologue of the T lymphocyte antigen CD28. Molecular cloning
RT and cell surface expression.";
RL J. Immunol. 144:3201-3210(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 156-218.
RX PubMed=1713603;
RA Harper K., Balzano C., Rouvier E., Mattei M.-G., Luciani M.-F.,
RA Golstein P.;
RT "CTLA-4 and CD28 activated lymphocyte molecules are closely related in both
RT mouse and human as to sequence, message expression, gene structure, and
RT chromosomal location.";
RL J. Immunol. 147:1037-1044(1991).
CC -!- FUNCTION: Involved in T-cell activation, the induction of cell
CC proliferation and cytokine production and promotion of T-cell survival.
CC Enhances the production of IL4 and IL10 in T-cells in conjunction with
CC TCR/CD3 ligation and CD40L costimulation.
CC {ECO:0000250|UniProtKB:P10747}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to
CC CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
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DR EMBL; M34563; AAA37395.1; -; mRNA.
DR EMBL; AL646054; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL672024; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR936842; CAM13249.1; -; Genomic_DNA.
DR EMBL; CH466548; EDL00156.1; -; Genomic_DNA.
DR EMBL; BC064058; AAH64058.1; -; mRNA.
DR EMBL; M74361; AAA37396.1; -; mRNA.
DR CCDS; CCDS14992.1; -.
DR PIR; A43523; A43523.
DR PIR; I49584; I49584.
DR RefSeq; NP_031668.3; NM_007642.4.
DR PDB; 2NAE; NMR; -; A=177-218.
DR PDBsum; 2NAE; -.
DR AlphaFoldDB; P31041; -.
DR SMR; P31041; -.
DR DIP; DIP-60858N; -.
DR IntAct; P31041; 3.
DR STRING; 10090.ENSMUSP00000027165; -.
DR GlyGen; P31041; 4 sites.
DR iPTMnet; P31041; -.
DR PhosphoSitePlus; P31041; -.
DR SwissPalm; P31041; -.
DR EPD; P31041; -.
DR PaxDb; P31041; -.
DR PeptideAtlas; P31041; -.
DR PRIDE; P31041; -.
DR ProteomicsDB; 280017; -.
DR Antibodypedia; 19958; 2966 antibodies from 48 providers.
DR DNASU; 12487; -.
DR Ensembl; ENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012.
DR GeneID; 12487; -.
DR KEGG; mmu:12487; -.
DR UCSC; uc007bev.2; mouse.
DR CTD; 940; -.
DR MGI; MGI:88327; Cd28.
DR VEuPathDB; HostDB:ENSMUSG00000026012; -.
DR eggNOG; ENOG502SAVP; Eukaryota.
DR GeneTree; ENSGT00530000063873; -.
DR HOGENOM; CLU_085095_1_0_1; -.
DR InParanoid; P31041; -.
DR OMA; CNYTYNG; -.
DR OrthoDB; 1222373at2759; -.
DR PhylomeDB; P31041; -.
DR TreeFam; TF335679; -.
DR Reactome; R-MMU-1257604; PIP3 activates AKT signaling.
DR Reactome; R-MMU-389356; CD28 co-stimulation.
DR Reactome; R-MMU-389357; CD28 dependent PI3K/Akt signaling.
DR Reactome; R-MMU-389359; CD28 dependent Vav1 pathway.
DR Reactome; R-MMU-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
DR BioGRID-ORCS; 12487; 2 hits in 112 CRISPR screens.
DR PRO; PR:P31041; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P31041; protein.
DR Bgee; ENSMUSG00000026012; Expressed in thymus and 55 other tissues.
DR Genevisible; P31041; MM.
DR GO; GO:0009986; C:cell surface; ISO:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0001772; C:immunological synapse; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045121; C:membrane raft; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0098636; C:protein complex involved in cell adhesion; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0019901; F:protein kinase binding; IPI:MGI.
DR GO; GO:0097190; P:apoptotic signaling pathway; IDA:MGI.
DR GO; GO:0035739; P:CD4-positive, alpha-beta T cell proliferation; IDA:MGI.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR GO; GO:0045060; P:negative thymic T cell selection; IMP:MGI.
DR GO; GO:0014065; P:phosphatidylinositol 3-kinase signaling; IDA:MGI.
DR GO; GO:2000563; P:positive regulation of CD4-positive, alpha-beta T cell proliferation; IDA:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0002863; P:positive regulation of inflammatory response to antigenic stimulus; IMP:MGI.
DR GO; GO:0032733; P:positive regulation of interleukin-10 production; ISS:UniProtKB.
DR GO; GO:0032743; P:positive regulation of interleukin-2 production; IMP:MGI.
DR GO; GO:0032753; P:positive regulation of interleukin-4 production; ISS:UniProtKB.
DR GO; GO:0048304; P:positive regulation of isotype switching to IgG isotypes; IMP:MGI.
DR GO; GO:0045840; P:positive regulation of mitotic nuclear division; ISS:UniProtKB.
DR GO; GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase signaling; IDA:MGI.
DR GO; GO:0051897; P:positive regulation of protein kinase B signaling; IDA:MGI.
DR GO; GO:0042102; P:positive regulation of T cell proliferation; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0043491; P:protein kinase B signaling; IDA:MGI.
DR GO; GO:0045589; P:regulation of regulatory T cell differentiation; IGI:MGI.
DR GO; GO:0045066; P:regulatory T cell differentiation; ISO:MGI.
DR GO; GO:0042110; P:T cell activation; ISO:MGI.
DR GO; GO:0031295; P:T cell costimulation; IDA:MGI.
DR GO; GO:0042098; P:T cell proliferation; IDA:MGI.
DR GO; GO:0050852; P:T cell receptor signaling pathway; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IGI:MGI.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR008093; CD28.
DR InterPro; IPR040216; CTLA4/CD28.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013106; Ig_V-set.
DR PANTHER; PTHR11494; PTHR11494; 1.
DR Pfam; PF15910; V-set_2; 1.
DR PRINTS; PR01717; CD28ANTIGEN.
DR SUPFAM; SSF48726; SSF48726; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000250"
FT CHAIN 20..218
FT /note="T-cell-specific surface glycoprotein CD28"
FT /id="PRO_0000014653"
FT TOPO_DOM 20..150
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 29..138
FT /note="Ig-like V-type"
FT MOD_RES 187
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P10747"
FT MOD_RES 189
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P10747"
FT MOD_RES 207
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P10747"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 41..113
FT /evidence="ECO:0000250"
FT DISULFID 67..87
FT /evidence="ECO:0000250"
FT CONFLICT 187..189
FT /note="SDY -> VTT (in Ref. 1; AAA37395)"
FT /evidence="ECO:0000305"
FT STRAND 185..187
FT /evidence="ECO:0007829|PDB:2NAE"
FT TURN 214..216
FT /evidence="ECO:0007829|PDB:2NAE"
SQ SEQUENCE 218 AA; 25243 MW; C8E1339DD7E53CA6 CRC64;
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK EFRASLYKGV
NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL WNLHVNHTDI YFCKIEFMYP
PPYLDNERSN GTIIHIKEKH LCHTQSSPKL FWALVVVAGV LFCYGLLVTV ALCVIWTNSR
RNRLLQSDYM NMTPRRPGLT RKPYQPYAPA RDFAAYRP
